ENSG00000008277

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000265727 ENSG00000008277 HEK293_OSMI2_6hA HEK293_TMG_6hB ADAM22 protein_coding protein_coding 3.100867 1.208185 6.373195 0.1929568 0.1598426 2.389545 0.13302237 0.00000000 0.00000000 0.00000000 0.00000000 0.00000000 0.05253750 0.00000000 0.000000000 0.000000000   0.001401471 FALSE TRUE
ENST00000398204 ENSG00000008277 HEK293_OSMI2_6hA HEK293_TMG_6hB ADAM22 protein_coding protein_coding 3.100867 1.208185 6.373195 0.1929568 0.1598426 2.389545 0.63674946 0.61820896 0.64996291 0.17305825 0.17984209 0.07114044 0.26161250 0.49316667 0.102466667 -0.390700000 0.001401471 0.001401471 FALSE TRUE
ENST00000398209 ENSG00000008277 HEK293_OSMI2_6hA HEK293_TMG_6hB ADAM22 protein_coding protein_coding 3.100867 1.208185 6.373195 0.1929568 0.1598426 2.389545 0.29407365 0.02139449 0.35609013 0.02139449 0.18181422 3.54361544 0.11199167 0.01536667 0.057333333 0.041966667 0.881096394 0.001401471 FALSE TRUE
ENST00000476330 ENSG00000008277 HEK293_OSMI2_6hA HEK293_TMG_6hB ADAM22 protein_coding processed_transcript 3.100867 1.208185 6.373195 0.1929568 0.1598426 2.389545 0.07290566 0.05796086 0.02303757 0.03010274 0.02303757 -1.04059658 0.02958750 0.05820000 0.003733333 -0.054466667 0.355741911 0.001401471   FALSE
MSTRG.30194.10 ENSG00000008277 HEK293_OSMI2_6hA HEK293_TMG_6hB ADAM22 protein_coding   3.100867 1.208185 6.373195 0.1929568 0.1598426 2.389545 0.25661352 0.20707659 0.31967667 0.20707659 0.31967667 0.60284766 0.06500833 0.14866667 0.047833333 -0.100833333 0.903283626 0.001401471 FALSE TRUE
MSTRG.30194.12 ENSG00000008277 HEK293_OSMI2_6hA HEK293_TMG_6hB ADAM22 protein_coding   3.100867 1.208185 6.373195 0.1929568 0.1598426 2.389545 0.40716016 0.06306905 0.41105019 0.03231065 0.27317225 2.52665982 0.12879167 0.05866667 0.062533333 0.003866667 1.000000000 0.001401471 FALSE TRUE
MSTRG.30194.13 ENSG00000008277 HEK293_OSMI2_6hA HEK293_TMG_6hB ADAM22 protein_coding   3.100867 1.208185 6.373195 0.1929568 0.1598426 2.389545 0.52624691 0.09817954 2.43128170 0.09817954 0.46478956 4.49613923 0.09029583 0.06966667 0.385500000 0.315833333 0.192555119 0.001401471 FALSE TRUE
MSTRG.30194.4 ENSG00000008277 HEK293_OSMI2_6hA HEK293_TMG_6hB ADAM22 protein_coding   3.100867 1.208185 6.373195 0.1929568 0.1598426 2.389545 0.48627304 0.04630078 1.55889661 0.02353227 0.30659003 4.80045164 0.14959583 0.04596667 0.243166667 0.197200000 0.046153459 0.001401471 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000008277 E001 0.1614157 0.034827806 1.000000e+00   7 87934143 87934250 108 + 0.066 0.000 -7.764
ENSG00000008277 E002 2.9217187 0.007719262 3.166267e-04 0.0038978252 7 87934251 87934328 78 + 0.334 0.899 2.584
ENSG00000008277 E003 3.2359199 0.007827058 4.419636e-06 0.0001034679 7 87934329 87934346 18 + 0.299 0.992 3.155
ENSG00000008277 E004 4.1340915 0.006778989 7.317303e-05 0.0011523056 7 87934347 87934386 40 + 0.450 1.019 2.380
ENSG00000008277 E005 4.0374750 0.036420076 1.329219e-03 0.0123783702 7 87934387 87934419 33 + 0.475 0.988 2.139
ENSG00000008277 E006 4.9709958 0.042440005 6.829876e-03 0.0438437314 7 87934420 87934465 46 + 0.581 1.014 1.730
ENSG00000008277 E007 6.3649934 0.005510339 1.161244e-02 0.0648611566 7 87934466 87934550 85 + 0.710 1.044 1.287
ENSG00000008277 E008 0.0000000       7 87934756 87935025 270 +      
ENSG00000008277 E009 3.6597304 0.006746818 5.839068e-01 0.7595608782 7 87935026 87935028 3 + 0.581 0.679 0.429
ENSG00000008277 E010 14.3427206 0.002165897 8.511826e-03 0.0516589188 7 87935029 87935186 158 + 1.038 1.299 0.933
ENSG00000008277 E011 3.5650168 0.030711132 9.185269e-01 0.9614642345 7 87964634 87964684 51 + 0.597 0.618 0.089
ENSG00000008277 E012 1.7191574 0.013080951 7.618718e-02 0.2371134946 7 87971294 87971375 82 + 0.299 0.618 1.672
ENSG00000008277 E013 15.2791111 0.003345933 2.006535e-01 0.4305461717 7 87978336 87978412 77 + 1.104 1.240 0.483
ENSG00000008277 E014 9.0783800 0.014793996 4.960906e-01 0.6982613717 7 88075626 88075629 4 + 0.949 0.863 -0.328
ENSG00000008277 E015 15.2613957 0.002803613 4.375764e-01 0.6569219710 7 88075630 88075692 63 + 1.142 1.069 -0.264
ENSG00000008277 E016 0.0000000       7 88089919 88090014 96 +      
ENSG00000008277 E017 22.2079266 0.001689002 4.340050e-02 0.1635895417 7 88108176 88108258 83 + 1.315 1.134 -0.640
ENSG00000008277 E018 21.8186167 0.001803587 3.062304e-04 0.0037926614 7 88114584 88114647 64 + 1.332 0.963 -1.321
ENSG00000008277 E019 0.0000000       7 88114648 88114648 1 +      
ENSG00000008277 E020 20.5084730 0.001604630 2.575825e-03 0.0208183111 7 88116745 88116814 70 + 1.297 0.992 -1.092
ENSG00000008277 E021 23.3816169 0.001966716 1.126807e-01 0.3036264005 7 88125589 88125659 71 + 1.325 1.190 -0.475
ENSG00000008277 E022 20.2611245 0.005747266 5.595641e-01 0.7429893199 7 88128602 88128676 75 + 1.255 1.207 -0.168
ENSG00000008277 E023 18.9802138 0.002087075 2.204205e-01 0.4540571042 7 88130388 88130459 72 + 1.243 1.133 -0.390
ENSG00000008277 E024 30.4516757 0.005540351 1.083008e-01 0.2962124215 7 88131269 88131435 167 + 1.436 1.312 -0.432
ENSG00000008277 E025 0.0000000       7 88131436 88132804 1369 +      
ENSG00000008277 E026 23.7131417 0.004472919 1.450203e-01 0.3549427019 7 88132867 88132951 85 + 1.333 1.207 -0.441
ENSG00000008277 E027 0.3206185 0.027442404 1.000000e+00   7 88132952 88132982 31 + 0.124 0.000 -11.400
ENSG00000008277 E028 22.7826556 0.001754512 9.686607e-01 0.9861630740 7 88134329 88134419 91 + 1.286 1.299 0.045
ENSG00000008277 E029 16.6173962 0.010204167 6.741898e-01 0.8190400143 7 88135980 88136031 52 + 1.176 1.134 -0.149
ENSG00000008277 E030 22.3456893 0.002018881 2.983928e-01 0.5388325519 7 88143026 88143125 100 + 1.307 1.224 -0.294
ENSG00000008277 E031 21.8834163 0.004459821 4.930549e-01 0.6960097786 7 88145125 88145196 72 + 1.293 1.240 -0.186
ENSG00000008277 E032 23.2010562 0.025846562 3.495262e-01 0.5868218813 7 88145415 88145507 93 + 1.324 1.223 -0.353
ENSG00000008277 E033 24.8733689 0.005772224 7.220170e-01 0.8492879700 7 88148977 88149057 81 + 1.334 1.313 -0.074
ENSG00000008277 E034 20.3713462 0.005116730 5.043061e-01 0.7038071489 7 88150981 88151031 51 + 1.262 1.207 -0.194
ENSG00000008277 E035 23.9194015 0.002092853 9.703032e-01 0.9870068009 7 88151257 88151320 64 + 1.311 1.326 0.050
ENSG00000008277 E036 29.0896541 0.001497456 7.987223e-01 0.8955533754 7 88153221 88153326 106 + 1.388 1.418 0.105
ENSG00000008277 E037 28.2680545 0.006707922 1.945285e-01 0.4227559583 7 88155887 88156006 120 + 1.402 1.299 -0.358
ENSG00000008277 E038 15.0202798 0.006146439 5.965058e-01 0.7680063369 7 88163012 88163030 19 + 1.137 1.091 -0.164
ENSG00000008277 E039 28.3570523 0.001441538 1.651939e-01 0.3842073534 7 88163031 88163180 150 + 1.402 1.299 -0.359
ENSG00000008277 E040 28.5706567 0.001324897 1.352539e-01 0.3401284598 7 88165832 88165946 115 + 1.411 1.299 -0.389
ENSG00000008277 E041 26.0508962 0.001587593 4.138779e-01 0.6388938639 7 88168137 88168227 91 + 1.358 1.299 -0.205
ENSG00000008277 E042 15.2213884 0.010290094 9.503069e-01 0.9771903713 7 88171544 88171561 18 + 1.122 1.134 0.044
ENSG00000008277 E043 0.5117739 0.023457187 6.026167e-01   7 88178935 88179042 108 + 0.124 0.212 0.933
ENSG00000008277 E044 0.6731895 0.053856921 8.466778e-01   7 88179043 88179129 87 + 0.175 0.213 0.347
ENSG00000008277 E045 23.5018626 0.002016898 4.384916e-01 0.6576405247 7 88181505 88181605 101 + 1.315 1.256 -0.208
ENSG00000008277 E046 18.6387432 0.011981676 8.049016e-01 0.8991514343 7 88181958 88182004 47 + 1.211 1.190 -0.075
ENSG00000008277 E047 12.8679029 0.011577361 9.157266e-01 0.9600467565 7 88182005 88182024 20 + 1.052 1.069 0.063
ENSG00000008277 E048 3.4200991 0.007515288 7.640203e-01 0.8750655662 7 88182025 88183554 1530 + 0.561 0.619 0.256
ENSG00000008277 E049 1.4161025 0.081055780 8.979984e-01 0.9508123236 7 88184292 88184402 111 + 0.332 0.355 0.142
ENSG00000008277 E050 1.3689166 0.021028362 2.952648e-01   7 88185830 88186614 785 + 0.262 0.462 1.199
ENSG00000008277 E051 3.6225933 0.017051388 3.300313e-01 0.5689637322 7 88186615 88186701 87 + 0.562 0.732 0.730
ENSG00000008277 E052 23.8876863 0.015738017 5.070934e-01 0.7057353753 7 88193116 88193239 124 + 1.295 1.362 0.234
ENSG00000008277 E053 37.6719804 0.039912033 9.728451e-01 0.9883934992 7 88196471 88196735 265 + 1.501 1.493 -0.026
ENSG00000008277 E054 31.2360605 0.002386735 5.158608e-01 0.7121639608 7 88196736 88197144 409 + 1.408 1.468 0.205
ENSG00000008277 E055 13.4654433 0.005172569 1.322905e-01 0.3353931637 7 88197145 88197239 95 + 1.038 1.207 0.607
ENSG00000008277 E056 166.2858429 0.002354366 8.955803e-01 0.9495077324 7 88197240 88200118 2879 + 2.134 2.138 0.013
ENSG00000008277 E057 64.4857353 0.014969061 1.971887e-04 0.0026430292 7 88200119 88203062 2944 + 1.656 1.924 0.907