ENSG00000005436

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000321027 ENSG00000005436 HEK293_OSMI2_6hA HEK293_TMG_6hB GCFC2 protein_coding protein_coding 10.77845 5.603808 16.9813 0.2900471 0.6050191 1.597745 0.7578571 0.1262363 0.5655441 0.12623625 0.37010523 2.0788157 0.05605000 0.02166667 0.034600000 0.012933333 0.87204684 0.003306715 FALSE TRUE
ENST00000427862 ENSG00000005436 HEK293_OSMI2_6hA HEK293_TMG_6hB GCFC2 protein_coding protein_coding 10.77845 5.603808 16.9813 0.2900471 0.6050191 1.597745 2.1435452 1.6428281 3.2256421 0.27213867 0.11197616 0.9691153 0.23872083 0.29423333 0.190800000 -0.103433333 0.43838600 0.003306715 FALSE TRUE
ENST00000470197 ENSG00000005436 HEK293_OSMI2_6hA HEK293_TMG_6hB GCFC2 protein_coding retained_intron 10.77845 5.603808 16.9813 0.2900471 0.6050191 1.597745 1.6610190 1.1355578 2.9652743 0.13607932 0.08210839 1.3769724 0.16652917 0.20166667 0.175066667 -0.026600000 0.84569766 0.003306715 FALSE TRUE
ENST00000470503 ENSG00000005436 HEK293_OSMI2_6hA HEK293_TMG_6hB GCFC2 protein_coding protein_coding 10.77845 5.603808 16.9813 0.2900471 0.6050191 1.597745 1.2446662 0.5494580 1.8405272 0.12709589 0.10921929 1.7258346 0.11483333 0.09613333 0.108700000 0.012566667 0.94234025 0.003306715 FALSE TRUE
ENST00000472230 ENSG00000005436 HEK293_OSMI2_6hA HEK293_TMG_6hB GCFC2 protein_coding nonsense_mediated_decay 10.77845 5.603808 16.9813 0.2900471 0.6050191 1.597745 1.6045721 0.3217365 3.6749213 0.05595806 0.79395682 3.4735242 0.11286667 0.05823333 0.214500000 0.156266667 0.02658944 0.003306715 FALSE TRUE
ENST00000492826 ENSG00000005436 HEK293_OSMI2_6hA HEK293_TMG_6hB GCFC2 protein_coding protein_coding 10.77845 5.603808 16.9813 0.2900471 0.6050191 1.597745 0.4761094 0.7085673 0.1231794 0.36996218 0.12317941 -2.4317522 0.06438333 0.12806667 0.006766667 -0.121300000 0.50322977 0.003306715   FALSE
MSTRG.18724.6 ENSG00000005436 HEK293_OSMI2_6hA HEK293_TMG_6hB GCFC2 protein_coding   10.77845 5.603808 16.9813 0.2900471 0.6050191 1.597745 2.1348159 1.0101184 3.0205768 0.12096016 0.19849177 1.5708558 0.18152917 0.17893333 0.177566667 -0.001366667 1.00000000 0.003306715 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000005436 E001 0.1308682 0.0326491905 0.1509070668   2 75652000 75652187 188 - 0.000 0.199 13.183
ENSG00000005436 E002 0.8899192 1.4158798172 0.8838905915   2 75662705 75662705 1 - 0.294 0.000 -12.265
ENSG00000005436 E003 0.8899192 1.4158798172 0.8838905915   2 75662706 75662707 2 - 0.294 0.000 -12.265
ENSG00000005436 E004 84.5489439 0.0615050503 0.6408752294 0.797457946 2 75662708 75664417 1710 - 1.845 1.918 0.247
ENSG00000005436 E005 11.8265155 0.0922385715 0.2302210831 0.465383109 2 75664418 75664418 1 - 1.083 0.871 -0.791
ENSG00000005436 E006 38.6291601 0.0507817855 0.1332312482 0.336878066 2 75664419 75664561 143 - 1.471 1.674 0.694
ENSG00000005436 E007 29.7406599 0.0288839973 0.0083140789 0.050772142 2 75664562 75664633 72 - 1.327 1.624 1.022
ENSG00000005436 E008 15.6065290 0.0026439352 0.0008461482 0.008663658 2 75664634 75664644 11 - 1.049 1.375 1.157
ENSG00000005436 E009 53.2284302 0.0171732926 0.0275115392 0.119818740 2 75664645 75664783 139 - 1.611 1.807 0.663
ENSG00000005436 E010 49.8743587 0.0249927677 0.0228827614 0.105555322 2 75665929 75666053 125 - 1.580 1.794 0.725
ENSG00000005436 E011 71.7374061 0.9518976870 0.3071904827 0.547799607 2 75670138 75670284 147 - 1.703 1.999 0.997
ENSG00000005436 E012 60.7224979 0.0448124222 0.0213508092 0.100494969 2 75671950 75672016 67 - 1.649 1.907 0.873
ENSG00000005436 E013 69.0964147 0.0081624758 0.0428842395 0.162253096 2 75673444 75673520 77 - 1.740 1.886 0.494
ENSG00000005436 E014 84.2104548 0.0004711840 0.6149475676 0.780250260 2 75680193 75680314 122 - 1.854 1.904 0.168
ENSG00000005436 E015 91.9774557 0.0004292348 0.4658367685 0.677193952 2 75687827 75687977 151 - 1.890 1.949 0.196
ENSG00000005436 E016 94.2930989 0.0009757151 0.1290438351 0.330273103 2 75689026 75689225 200 - 1.923 1.885 -0.130
ENSG00000005436 E017 69.1570979 0.0177417874 0.1387796860 0.345648802 2 75689969 75690081 113 - 1.804 1.698 -0.357
ENSG00000005436 E018 1.2496975 0.1757998445 0.8473571422   2 75690488 75690637 150 - 0.312 0.339 0.173
ENSG00000005436 E019 48.8446385 0.9280691618 0.7323513803 0.855635954 2 75690638 75690719 82 - 1.645 1.582 -0.213
ENSG00000005436 E020 60.6340398 0.0220046723 0.5347729331 0.725477655 2 75691977 75692100 124 - 1.735 1.695 -0.137
ENSG00000005436 E021 71.6614529 0.0122584421 0.1120617209 0.302524231 2 75694241 75694427 187 - 1.814 1.736 -0.262
ENSG00000005436 E022 0.0000000       2 75694428 75694479 52 -      
ENSG00000005436 E023 36.9168616 0.0256478175 0.1206660423 0.316922230 2 75696200 75696251 52 - 1.540 1.419 -0.416
ENSG00000005436 E024 43.1526549 0.0133890590 0.1597191529 0.376379288 2 75696252 75696315 64 - 1.597 1.516 -0.279
ENSG00000005436 E025 72.4444996 0.0102480984 0.0002292421 0.002992588 2 75701190 75701287 98 - 1.844 1.618 -0.768
ENSG00000005436 E026 1.9013457 0.1874616142 0.6414963920 0.797826695 2 75701288 75701372 85 - 0.443 0.335 -0.609
ENSG00000005436 E027 34.0378698 0.0178005944 0.7285678304 0.853358356 2 75701796 75702198 403 - 1.460 1.526 0.228
ENSG00000005436 E028 95.6904695 0.0046689210 0.0089521985 0.053606812 2 75702199 75702312 114 - 1.943 1.840 -0.347
ENSG00000005436 E029 94.4955433 0.0064516464 0.0009484270 0.009490026 2 75702313 75702423 111 - 1.949 1.793 -0.523
ENSG00000005436 E030 6.5257074 0.0051683268 0.0489291794 0.177072794 2 75702424 75702447 24 - 0.872 0.592 -1.147
ENSG00000005436 E031 59.4091908 0.0008604099 0.0010078065 0.009971959 2 75706523 75706651 129 - 1.750 1.595 -0.526
ENSG00000005436 E032 0.9221142 0.0172671820 0.0329075262   2 75710444 75710590 147 - 0.133 0.522 2.702
ENSG00000005436 E033 40.3059552 0.0045559487 0.8289395233 0.913226791 2 75710591 75712062 1472 - 1.555 1.567 0.043
ENSG00000005436 E034 1.7798384 1.8996606912 0.9118186064 0.958062262 2 75712223 75713155 933 - 0.473 0.000 -13.289