ENSG00000005020

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000345317 ENSG00000005020 HEK293_OSMI2_6hA HEK293_TMG_6hB SKAP2 protein_coding protein_coding 8.510438 2.992022 12.76483 0.7600897 0.38948 2.089298 6.555006 2.944154 9.114487 0.8076775 0.1914205 1.626999 0.8507333 0.9677 0.7154667 -0.2522333 0.0683926677 0.0001407411 FALSE TRUE
MSTRG.29601.3 ENSG00000005020 HEK293_OSMI2_6hA HEK293_TMG_6hB SKAP2 protein_coding   8.510438 2.992022 12.76483 0.7600897 0.38948 2.089298 1.580271 0.000000 2.604316 0.0000000 0.9165970 8.030290 0.1126292 0.0000 0.2026333 0.2026333 0.0001407411 0.0001407411 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000005020 E001 299.7355297 0.0005367973 3.283611e-08 1.367753e-06 7 26667068 26668964 1897 - 2.385 2.501 0.387
ENSG00000005020 E002 33.8840741 0.0136749947 6.280990e-01 7.893107e-01 7 26668965 26669104 140 - 1.495 1.455 -0.136
ENSG00000005020 E003 59.2278156 0.0121720736 4.370602e-01 6.564795e-01 7 26669105 26669656 552 - 1.706 1.752 0.157
ENSG00000005020 E004 55.5693602 0.0020242732 3.816625e-02 1.500349e-01 7 26670091 26670192 102 - 1.718 1.597 -0.412
ENSG00000005020 E005 65.9635996 0.0032749445 5.407233e-01 7.295404e-01 7 26684736 26684848 113 - 1.769 1.735 -0.116
ENSG00000005020 E006 42.8108187 0.0011197073 6.702641e-01 8.164693e-01 7 26690285 26690306 22 - 1.570 1.597 0.092
ENSG00000005020 E007 49.9977890 0.0008275844 3.375475e-01 5.759286e-01 7 26690307 26690356 50 - 1.632 1.687 0.186
ENSG00000005020 E008 30.3038803 0.0015365944 5.318010e-01 7.233047e-01 7 26690357 26690362 6 - 1.420 1.468 0.163
ENSG00000005020 E009 64.8078523 0.0010314498 5.577769e-01 7.416833e-01 7 26725428 26725565 138 - 1.746 1.778 0.108
ENSG00000005020 E010 39.4749898 0.0010669855 2.390751e-01 4.755448e-01 7 26725923 26725986 64 - 1.566 1.492 -0.254
ENSG00000005020 E011 0.3337900 0.0514091819 4.017885e-01   7 26726682 26726881 200 - 0.073 0.195 1.629
ENSG00000005020 E012 24.9299814 0.0018097922 7.008413e-01 8.361906e-01 7 26726882 26726888 7 - 1.369 1.341 -0.096
ENSG00000005020 E013 57.4967177 0.0008076457 8.093904e-02 2.466898e-01 7 26726889 26727006 118 - 1.731 1.638 -0.315
ENSG00000005020 E014 38.1092660 0.0010203746 2.264979e-01 4.612017e-01 7 26738795 26738839 45 - 1.553 1.476 -0.264
ENSG00000005020 E015 36.9787201 0.0009940533 5.199806e-01 7.149771e-01 7 26738840 26738878 39 - 1.532 1.492 -0.138
ENSG00000005020 E016 46.1281622 0.0088016375 2.342160e-01 4.699679e-01 7 26739887 26739964 78 - 1.637 1.543 -0.324
ENSG00000005020 E017 0.0000000       7 26742342 26742475 134 -      
ENSG00000005020 E018 0.0000000       7 26746567 26746686 120 -      
ENSG00000005020 E019 0.0000000       7 26844029 26844029 1 -      
ENSG00000005020 E020 46.8345059 0.0010958500 1.742631e-02 8.702292e-02 7 26844030 26844137 108 - 1.654 1.507 -0.499
ENSG00000005020 E021 0.0000000       7 26847996 26848068 73 -      
ENSG00000005020 E022 26.1943979 0.0123167821 1.337556e-01 3.376940e-01 7 26854137 26854162 26 - 1.409 1.256 -0.533
ENSG00000005020 E023 38.3756683 0.0013913046 2.342018e-01 4.699573e-01 7 26854785 26854890 106 - 1.555 1.476 -0.269
ENSG00000005020 E024 0.0000000       7 26855118 26855151 34 -      
ENSG00000005020 E025 1.4217120 0.0121989077 3.241376e-01 5.634477e-01 7 26857451 26857678 228 - 0.392 0.195 -1.371
ENSG00000005020 E026 0.0000000       7 26857920 26858159 240 -      
ENSG00000005020 E027 39.2341486 0.0009343785 6.164779e-02 2.065003e-01 7 26864363 26864608 246 - 1.572 1.450 -0.418
ENSG00000005020 E028 0.6765862 0.2239325185 3.850131e-01   7 26864842 26865113 272 - 0.242 0.000 -9.733
ENSG00000005020 E029 0.1779838 0.0387582735 1.000000e+00   7 26893839 26893973 135 - 0.073 0.000 -8.456
ENSG00000005020 E030 0.0000000       7 26995155 26995239 85 -