ENSG00000286451

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000652950 ENSG00000286451 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA lncRNA 2.038484 1.230716 2.2704 0.112329 0.1688766 0.8781132 1.5136751 0.9983619 1.7135083 0.18141822 0.03481579 0.7733348 0.7539625 0.7982333 0.7634667 -0.03476667 0.897545041 0.001512048   FALSE
MSTRG.32907.1 ENSG00000286451 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA   2.038484 1.230716 2.2704 0.112329 0.1688766 0.8781132 0.2487054 0.0000000 0.2815977 0.00000000 0.07691720 4.8659073 0.1214208 0.0000000 0.1201667 0.12016667 0.001512048 0.001512048   FALSE
MSTRG.32907.2 ENSG00000286451 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA   2.038484 1.230716 2.2704 0.112329 0.1688766 0.8781132 0.2761033 0.2323546 0.2752937 0.07123122 0.10109907 0.2353283 0.1246167 0.2017667 0.1163667 -0.08540000 0.659038396 0.001512048   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000286451 E001 2.291692 0.006896908 0.00829169 0.02452247 9 83063243 83063245 3 + 0.642 0.158 -2.950
ENSG00000286451 E002 5.500310 0.003615506 0.21008916 0.33864842 9 83063246 83063397 152 + 0.863 0.694 -0.673
ENSG00000286451 E003 2.475470 0.007562647 0.86754639 0.91883102 9 83063398 83063413 16 + 0.534 0.504 -0.142
ENSG00000286451 E004 2.070244 0.007611136 0.33687397 0.48073340 9 83063414 83063418 5 + 0.534 0.364 -0.879
ENSG00000286451 E005 4.785610 0.018487320 0.13965733 0.24755012 9 83067903 83068064 162 + 0.833 0.609 -0.920
ENSG00000286451 E006 3.101126 0.004969946 0.31330407 0.45586836 9 83068065 83068066 2 + 0.665 0.504 -0.728
ENSG00000286451 E007 35.061712 0.001219786 0.12122254 0.22167227 9 83068067 83068828 762 + 1.518 1.583 0.223
ENSG00000286451 E008 19.335084 0.001255827 0.10478097 0.19777782 9 83068829 83069143 315 + 1.254 1.360 0.372
ENSG00000286451 E009 0.000000       9 83071427 83071452 26 +