ENSG00000285184

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000642888 ENSG00000285184 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA lncRNA 3.775822 6.081008 2.283164 0.423557 0.2814248 -1.409341 0.1095223 0.03076894 0.1747792 0.03076894 0.08801151 2.1802603 0.04020000 0.005866667 0.08723333 0.08136667 3.157502e-01 9.677772e-06 FALSE FALSE
ENST00000648868 ENSG00000285184 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA lncRNA 3.775822 6.081008 2.283164 0.423557 0.2814248 -1.409341 0.2247180 0.23871776 0.4493049 0.05596032 0.12160187 0.8849427 0.07398333 0.039533333 0.19070000 0.15116667 1.556727e-04 9.677772e-06 FALSE FALSE
ENST00000687771 ENSG00000285184 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA lncRNA 3.775822 6.081008 2.283164 0.423557 0.2814248 -1.409341 3.2992643 5.63277635 1.4833812 0.41481417 0.24632509 -1.9178227 0.83967500 0.926000000 0.64516667 -0.28083333 9.677772e-06 9.677772e-06   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000285184 E001 10.6116242 0.0022799693 4.500455e-03 1.454374e-02 1 150045660 150046726 1067 - 1.222 0.932 -1.052
ENSG00000285184 E002 17.1719142 0.0247254687 8.760974e-05 4.638348e-04 1 150046727 150047945 1219 - 1.454 1.067 -1.365
ENSG00000285184 E003 3.2171659 0.0066505145 2.607917e-02 6.410562e-02 1 150048455 150048551 97 - 0.812 0.461 -1.538
ENSG00000285184 E004 2.5141045 0.0063922772 2.687484e-01 4.072363e-01 1 150050482 150050540 59 - 0.656 0.461 -0.900
ENSG00000285184 E005 1.3640887 0.0101257926 1.469333e-01 2.575010e-01 1 150054509 150054640 132 - 0.525 0.258 -1.538
ENSG00000285184 E006 0.0000000       1 150057428 150057603 176 -      
ENSG00000285184 E007 0.0000000       1 150058236 150058339 104 -      
ENSG00000285184 E008 0.4418608 0.0226817134 4.828547e-02 1.061359e-01 1 150066076 150066171 96 - 0.338 0.000 -11.194
ENSG00000285184 E009 0.1451727 0.0429579569 3.723805e-01   1 150066172 150066172 1 - 0.144 0.000 -9.732
ENSG00000285184 E010 0.4783925 0.0212880824 4.102287e-01 5.538289e-01 1 150066173 150066838 666 - 0.251 0.104 -1.536
ENSG00000285184 E011 1.7265394 0.0122942075 6.501775e-01 7.612648e-01 1 150066839 150066969 131 - 0.471 0.372 -0.533
ENSG00000285184 E012 3.4905434 0.0094852787 1.884752e-01 3.118106e-01 1 150066970 150067310 341 - 0.756 0.536 -0.948
ENSG00000285184 E013 20.7110959 0.0018636455 1.970798e-07 1.890248e-06 1 150067311 150067482 172 - 1.049 1.417 1.303
ENSG00000285184 E014 24.9345103 0.0009006728 2.422453e-07 2.285149e-06 1 150067483 150067722 240 - 1.179 1.487 1.075