ENSG00000278768

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000618857 ENSG00000278768 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA lncRNA 3.081472 4.549252 2.072979 0.6067628 0.1556615 -1.130149 0.3293873 1.412041 0.0000000 0.72923020 0.00000000 -7.15181962 0.0665375 0.2732667 0.0000 -0.2732667 0.20123489 0.02816557   FALSE
ENST00000649580 ENSG00000278768 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA retained_intron 3.081472 4.549252 2.072979 0.6067628 0.1556615 -1.130149 2.0556809 1.799220 1.9109385 0.15363302 0.03719106 0.08644324 0.7624792 0.4208667 0.9318 0.5109333 0.02816557 0.02816557 TRUE FALSE
ENST00000688366 ENSG00000278768 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA lncRNA 3.081472 4.549252 2.072979 0.6067628 0.1556615 -1.130149 0.6964039 1.337991 0.1620408 0.03188855 0.16204078 -2.96998816 0.1709833 0.3058667 0.0682 -0.2376667 0.12926242 0.02816557   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000278768 E001 119.839171 0.0161637571 3.851336e-05 0.0002228949 11 117288453 117290873 2421 + 2.203 1.959 -0.819
ENSG00000278768 E002 13.165393 0.0236558037 8.593209e-01 0.9132438580 11 117290874 117290929 56 + 1.159 1.107 -0.188
ENSG00000278768 E003 73.025383 0.0003915813 3.265804e-01 0.4700064556 11 117290930 117291702 773 + 1.846 1.845 -0.004
ENSG00000278768 E004 17.621914 0.0020443538 2.759771e-02 0.0672070169 11 117291703 117291771 69 + 1.376 1.162 -0.752
ENSG00000278768 E005 4.991128 0.0527880674 2.067632e-02 0.0529054422 11 117292176 117292210 35 + 0.482 0.859 1.616
ENSG00000278768 E006 13.448340 0.0015456375 8.246729e-01 0.8895559027 11 117292211 117292333 123 + 1.147 1.130 -0.060
ENSG00000278768 E007 43.478074 0.0005201062 5.791250e-02 0.1230528219 11 117292334 117292797 464 + 1.585 1.645 0.206
ENSG00000278768 E008 50.644469 0.0024105632 4.743918e-03 0.0152165157 11 117292798 117293074 277 + 1.620 1.730 0.373
ENSG00000278768 E009 51.074866 0.0166839821 3.373211e-04 0.0015296200 11 117293075 117293578 504 + 1.526 1.766 0.816