ENSG00000278053

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000617633 ENSG00000278053 HEK293_OSMI2_2hA HEK293_TMG_2hB DDX52 protein_coding protein_coding 17.62109 5.568575 29.29671 0.1258458 0.2426413 2.393263 3.998143 1.5437919 6.017954 0.1334037 0.3885460 1.955875 0.2512708 0.2784333 0.2053333 -0.0731000 0.232311774 0.001252772 FALSE TRUE
ENST00000620209 ENSG00000278053 HEK293_OSMI2_2hA HEK293_TMG_2hB DDX52 protein_coding nonsense_mediated_decay 17.62109 5.568575 29.29671 0.1258458 0.2426413 2.393263 4.842816 3.0393218 6.482659 0.3199797 1.0015720 1.090321 0.3327875 0.5488333 0.2217000 -0.3271333 0.002289414 0.001252772 FALSE TRUE
MSTRG.14218.3 ENSG00000278053 HEK293_OSMI2_2hA HEK293_TMG_2hB DDX52 protein_coding   17.62109 5.568575 29.29671 0.1258458 0.2426413 2.393263 7.492755 0.8955487 13.513308 0.5563579 0.5058909 3.900512 0.3454667 0.1566000 0.4611000 0.3045000 0.341158319 0.001252772 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000278053 E001 2.8943398 0.0943465920 1.721440e-01 2.909783e-01 17 37609739 37609786 48 - 0.465 0.760 1.311
ENSG00000278053 E002 15.6622062 0.0341003326 9.325715e-04 3.728220e-03 17 37609787 37609909 123 - 1.024 1.437 1.459
ENSG00000278053 E003 37.8648858 0.0163992375 9.649889e-04 3.838458e-03 17 37609910 37610157 248 - 1.444 1.731 0.978
ENSG00000278053 E004 166.9999464 0.0006671862 2.098502e-01 3.383763e-01 17 37610158 37612109 1952 - 2.159 2.197 0.128
ENSG00000278053 E005 105.3293175 0.0003054535 9.721314e-04 3.863381e-03 17 37612110 37613112 1003 - 2.000 1.859 -0.476
ENSG00000278053 E006 149.3288288 0.0136763506 2.659832e-01 4.041037e-01 17 37613113 37613754 642 - 2.099 2.176 0.258
ENSG00000278053 E007 226.9686102 0.0009961678 7.228936e-10 1.101416e-08 17 37613755 37614264 510 - 2.251 2.422 0.569
ENSG00000278053 E008 112.9672487 0.0003117265 3.795735e-04 1.695609e-03 17 37614265 37614353 89 - 1.963 2.091 0.429
ENSG00000278053 E009 119.4731354 0.0003049384 4.708715e-04 2.048716e-03 17 37618292 37618384 93 - 1.990 2.112 0.410
ENSG00000278053 E010 108.8482755 0.0003812159 3.893968e-02 8.907599e-02 17 37619768 37619839 72 - 1.963 2.039 0.256
ENSG00000278053 E011 5.8131243 0.0028150278 1.970534e-02 5.086268e-02 17 37619840 37620872 1033 - 0.850 0.452 -1.735
ENSG00000278053 E012 120.4409775 0.0003795854 6.316909e-02 1.319892e-01 17 37620873 37620948 76 - 2.009 2.074 0.219
ENSG00000278053 E013 3.0958155 0.0051573893 8.210509e-01 8.871947e-01 17 37620949 37621126 178 - 0.578 0.536 -0.191
ENSG00000278053 E014 159.6971038 0.0005065791 5.209511e-01 6.548772e-01 17 37621127 37621277 151 - 2.144 2.162 0.062
ENSG00000278053 E015 1.4768704 0.0693806759 6.424694e-02 1.337765e-01 17 37621278 37621397 120 - 0.437 0.000 -10.841
ENSG00000278053 E016 135.5084351 0.0003619966 8.029107e-01 8.744964e-01 17 37621398 37621520 123 - 2.074 2.081 0.021
ENSG00000278053 E017 3.0302407 0.0049944832 3.424205e-02 8.022276e-02 17 37624055 37624343 289 - 0.634 0.207 -2.439
ENSG00000278053 E018 101.6140779 0.0009106649 1.060032e-01 1.996162e-01 17 37624344 37624434 91 - 1.971 1.897 -0.247
ENSG00000278053 E019 142.4529164 0.0058113325 3.440835e-01 4.881353e-01 17 37625895 37626098 204 - 2.111 2.055 -0.185
ENSG00000278053 E020 60.8707727 0.0005105285 5.186510e-01 6.528869e-01 17 37626788 37626825 38 - 1.726 1.756 0.103
ENSG00000278053 E021 50.7979327 0.0008171056 6.296228e-01 7.449637e-01 17 37626826 37626860 35 - 1.665 1.634 -0.105
ENSG00000278053 E022 81.1805466 0.0019257555 2.345147e-02 5.868505e-02 17 37628561 37628672 112 - 1.885 1.765 -0.405
ENSG00000278053 E023 98.1523135 0.0027480012 1.263179e-02 3.504254e-02 17 37630030 37630173 144 - 1.968 1.840 -0.431
ENSG00000278053 E024 0.1817044 0.0407718413 1.247880e-01   17 37630809 37632112 1304 - 0.000 0.207 10.863
ENSG00000278053 E025 131.1741603 0.0002558678 4.622869e-05 2.626762e-04 17 37632113 37632298 186 - 2.099 1.942 -0.526
ENSG00000278053 E026 117.2656752 0.0002439599 3.421025e-08 3.810801e-07 17 37633288 37633418 131 - 2.064 1.833 -0.776
ENSG00000278053 E027 0.0000000       17 37635551 37635604 54 -      
ENSG00000278053 E028 1.0664556 0.0595323692 1.466043e-01 2.570654e-01 17 37639454 37639568 115 - 0.182 0.454 1.822
ENSG00000278053 E029 148.4063714 0.0095167607 1.090139e-06 8.976551e-06 17 37642110 37642308 199 - 2.176 1.874 -1.012
ENSG00000278053 E030 0.0000000       17 37642720 37642771 52 -      
ENSG00000278053 E031 74.7062804 0.0221736607 6.893062e-02 1.416006e-01 17 37643334 37643446 113 - 1.858 1.693 -0.556