ENSG00000277693

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000616952 ENSG00000277693 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA lncRNA 1.982108 3.485321 1.009 0.715181 0.3565824 -1.778271 1.6942226 3.2782414 0.3996289 0.68270101 0.3996289 -3.004927 0.7541750 0.93850000 0.2324 -0.7061000 0.022856228 0.001009794   FALSE
ENST00000622592 ENSG00000277693 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA lncRNA 1.982108 3.485321 1.009 0.715181 0.3565824 -1.778271 0.2349055 0.1077469 0.5697494 0.02907238 0.0396231 2.299740 0.2079917 0.03033333 0.7160 0.6856667 0.001009794 0.001009794 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000277693 E001 5.7323804 0.003813048 0.02117327 0.05396346 21 10328411 10328935 525 - 0.893 0.629 -1.066
ENSG00000277693 E002 2.6175367 0.006949209 0.12817002 0.23161122 21 10328936 10329038 103 - 0.622 0.380 -1.188
ENSG00000277693 E003 1.7391231 0.010921470 0.01292140 0.03572927 21 10336889 10338454 1566 - 0.226 0.630 2.257
ENSG00000277693 E004 1.3954090 0.011791424 0.30891894 0.45119627 21 10338455 10338566 112 - 0.281 0.457 1.037
ENSG00000277693 E005 0.9211837 0.014810658 0.98195459 0.99272302 21 10340414 10340478 65 - 0.281 0.286 0.033
ENSG00000277693 E006 2.3249987 0.007298972 0.41351325 0.55698773 21 10342616 10342669 54 - 0.450 0.579 0.620
ENSG00000277693 E007 1.2115146 0.015333858 0.57960789 0.70445694 21 10342670 10342690 21 - 0.281 0.380 0.622
ENSG00000277693 E008 0.7353121 0.020522696 0.48349978 0.62151463 21 10342691 10342737 47 - 0.163 0.286 1.038