ENSG00000277258

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000620225 ENSG00000277258 HEK293_OSMI2_2hA HEK293_TMG_2hB PCGF2 protein_coding protein_coding 21.47689 29.2087 16.72063 1.227313 0.8571168 -0.8044002 7.278860 12.490688 4.769294 0.07086905 0.1939918 -1.3871380 0.3249042 0.4291667 0.2870000 -0.1421666667 0.003541593 0.003541593 FALSE TRUE
MSTRG.14245.10 ENSG00000277258 HEK293_OSMI2_2hA HEK293_TMG_2hB PCGF2 protein_coding   21.47689 29.2087 16.72063 1.227313 0.8571168 -0.8044002 1.736172 1.324928 3.058096 1.32492753 0.8995570 1.2005821 0.1005875 0.0449000 0.1786333 0.1337333333 0.113035285 0.003541593 FALSE FALSE
MSTRG.14245.9 ENSG00000277258 HEK293_OSMI2_2hA HEK293_TMG_2hB PCGF2 protein_coding   21.47689 29.2087 16.72063 1.227313 0.8571168 -0.8044002 10.775570 13.603329 7.759240 0.67582187 0.1787501 -0.8091745 0.4958958 0.4668000 0.4664667 -0.0003333333 1.000000000 0.003541593 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000277258 E001 1.7660898 0.0083035036 0.1182684586 0.2174478079 17 38733898 38733899 2 - 0.174 0.468 1.980
ENSG00000277258 E002 156.2291695 0.0052756947 0.0023924136 0.0084373130 17 38733900 38734253 354 - 2.038 2.177 0.466
ENSG00000277258 E003 78.5196464 0.0028545380 0.0024153145 0.0085076248 17 38734254 38734279 26 - 1.733 1.885 0.514
ENSG00000277258 E004 251.0543706 0.0001817143 0.0000552553 0.0003079262 17 38734280 38735043 764 - 2.277 2.369 0.308
ENSG00000277258 E005 29.5035028 0.0079957535 0.7028565386 0.8016258551 17 38735044 38735108 65 - 1.408 1.432 0.082
ENSG00000277258 E006 14.8966415 0.0053848405 0.0358696202 0.0833309862 17 38735109 38735111 3 - 0.971 1.190 0.793
ENSG00000277258 E007 26.6326329 0.0008437106 0.0410773028 0.0930290348 17 38735112 38735135 24 - 1.272 1.422 0.523
ENSG00000277258 E008 293.3756154 0.0008558666 0.0003969530 0.0017626879 17 38735136 38735491 356 - 2.353 2.437 0.280
ENSG00000277258 E009 159.0731372 0.0016587731 0.8022440316 0.8740236943 17 38735492 38735564 73 - 2.160 2.145 -0.050
ENSG00000277258 E010 117.7570030 0.0021401285 0.5371361785 0.6687840531 17 38735565 38735574 10 - 2.042 2.011 -0.104
ENSG00000277258 E011 143.3544839 0.0007673699 0.6459020044 0.7579593347 17 38735575 38735600 26 - 2.121 2.099 -0.076
ENSG00000277258 E012 198.1204030 0.0006052661 0.2380771980 0.3719069134 17 38736090 38736170 81 - 2.274 2.233 -0.137
ENSG00000277258 E013 214.2376103 0.0014915252 0.0853262691 0.1679320753 17 38738353 38738448 96 - 2.321 2.261 -0.200
ENSG00000277258 E014 3.5707928 0.0058129420 0.3270053222 0.4704528887 17 38738449 38738451 3 - 0.734 0.580 -0.661
ENSG00000277258 E015 181.2631515 0.0015059498 0.0791556403 0.1581618406 17 38738541 38738595 55 - 2.254 2.189 -0.217
ENSG00000277258 E016 2.6476199 0.0087847686 0.8439190219 0.9028325212 17 38738749 38738752 4 - 0.539 0.499 -0.188
ENSG00000277258 E017 196.0712270 0.0101257146 0.3963238486 0.5401026087 17 38738753 38738861 109 - 2.279 2.225 -0.181
ENSG00000277258 E018 130.4851963 0.0027204982 0.7002273620 0.7997442202 17 38739068 38739118 51 - 2.079 2.055 -0.080
ENSG00000277258 E019 118.6946662 0.0050412667 0.4286508464 0.5713786335 17 38739198 38739253 56 - 2.057 2.010 -0.159
ENSG00000277258 E020 132.2227104 0.0055695887 0.0097664002 0.0281884714 17 38739586 38739682 97 - 2.163 2.031 -0.440
ENSG00000277258 E021 90.8309044 0.0103008864 0.0061993389 0.0191396330 17 38740291 38740442 152 - 2.032 1.854 -0.600
ENSG00000277258 E022 3.4581749 0.0061706467 0.1951255276 0.3201429484 17 38746468 38746628 161 - 0.734 0.528 -0.898
ENSG00000277258 E023 1.1479526 0.4859394385 0.7419118627 0.8309418126 17 38747470 38747552 83 - 0.396 0.268 -0.798
ENSG00000277258 E024 8.4282757 0.0051551208 0.6142822917 0.7327964417 17 38747879 38747971 93 - 0.897 0.952 0.208
ENSG00000277258 E025 7.5576204 0.0022077699 0.5057279987 0.6414612491 17 38747972 38748195 224 - 0.948 0.860 -0.333
ENSG00000277258 E026 3.3881685 0.0048461496 0.9337352000 0.9622501533 17 38748196 38748308 113 - 0.597 0.604 0.032
ENSG00000277258 E027 0.2934659 0.0286875603 0.5558835431   17 38748309 38748378 70 - 0.174 0.085 -1.193
ENSG00000277258 E028 7.6392670 0.0024019432 0.2009187024 0.3273345667 17 38749362 38751483 2122 - 0.993 0.834 -0.605