ENSG00000273559

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000611845 ENSG00000273559 HEK293_OSMI2_2hA HEK293_TMG_2hB CWC25 protein_coding retained_intron 7.540525 4.922873 9.828594 0.5010521 0.6241284 0.9960239 0.5783333 0.1758568 0.6106090 0.09395285 0.3468674 1.7394928 0.07366667 0.03336667 0.060000000 0.02663333 0.957907577 0.001336076   FALSE
ENST00000614790 ENSG00000273559 HEK293_OSMI2_2hA HEK293_TMG_2hB CWC25 protein_coding protein_coding 7.540525 4.922873 9.828594 0.5010521 0.6241284 0.9960239 5.4888404 4.1127677 7.1866637 0.39232961 0.4769967 0.8037151 0.73865000 0.83643333 0.733100000 -0.10333333 0.159055070 0.001336076 FALSE TRUE
ENST00000619299 ENSG00000273559 HEK293_OSMI2_2hA HEK293_TMG_2hB CWC25 protein_coding nonsense_mediated_decay 7.540525 4.922873 9.828594 0.5010521 0.6241284 0.9960239 0.3044640 0.3001592 0.0919224 0.10214019 0.0919224 -1.6055379 0.03656250 0.06593333 0.008433333 -0.05750000 0.142434816 0.001336076 FALSE TRUE
MSTRG.14249.3 ENSG00000273559 HEK293_OSMI2_2hA HEK293_TMG_2hB CWC25 protein_coding   7.540525 4.922873 9.828594 0.5010521 0.6241284 0.9960239 1.1614341 0.3340893 1.9393987 0.13172054 0.0963618 2.5021741 0.15031667 0.06420000 0.198466667 0.13426667 0.001336076 0.001336076 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000273559 E001 160.9358356 0.0002159245 5.571471e-05 0.0003102126 17 38800441 38801724 1284 - 2.161 2.267 0.353
ENSG00000273559 E002 73.9423504 0.0004026555 7.502285e-03 0.0225182660 17 38801725 38801813 89 - 1.826 1.933 0.361
ENSG00000273559 E003 120.7512348 0.0021197483 4.664405e-02 0.1031948568 17 38801814 38802206 393 - 2.054 2.125 0.239
ENSG00000273559 E004 66.9968924 0.0049833444 4.998122e-01 0.6360785420 17 38802700 38802814 115 - 1.845 1.806 -0.132
ENSG00000273559 E005 46.5164735 0.0004687370 8.529014e-02 0.1678714366 17 38802815 38802839 25 - 1.709 1.620 -0.304
ENSG00000273559 E006 38.9761742 0.0005240822 5.099535e-02 0.1109367233 17 38802840 38802861 22 - 1.643 1.532 -0.379
ENSG00000273559 E007 66.5421560 0.0004424622 4.333339e-01 0.5757373239 17 38806297 38806395 99 - 1.839 1.804 -0.116
ENSG00000273559 E008 4.8659482 0.0671373090 3.915611e-01 0.5354763594 17 38806503 38806764 262 - 0.829 0.678 -0.610
ENSG00000273559 E009 44.0848959 0.0036340257 9.161365e-01 0.9509786228 17 38806765 38806799 35 - 1.648 1.653 0.018
ENSG00000273559 E010 74.4923399 0.0007662961 6.175803e-01 0.7353600550 17 38806800 38806976 177 - 1.882 1.861 -0.071
ENSG00000273559 E011 39.0756010 0.0079128517 2.751576e-01 0.4144229626 17 38809702 38809765 64 - 1.628 1.548 -0.274
ENSG00000273559 E012 0.5943067 0.0234994446 6.166072e-01 0.7346274114 17 38809766 38809808 43 - 0.243 0.153 -0.824
ENSG00000273559 E013 3.7717172 0.0042571607 9.699803e-01 0.9852118277 17 38810276 38810353 78 - 0.676 0.682 0.025
ENSG00000273559 E014 2.4818392 0.0105219728 9.800297e-01 0.9915437712 17 38810354 38810400 47 - 0.544 0.549 0.023
ENSG00000273559 E015 3.8750502 0.0041192409 1.543387e-02 0.0414767112 17 38810401 38810467 67 - 0.813 0.430 -1.698
ENSG00000273559 E016 46.4480465 0.0067834381 6.960656e-01 0.7965132494 17 38810468 38810595 128 - 1.685 1.663 -0.077
ENSG00000273559 E017 41.2187382 0.0030689455 5.733723e-01 0.6993001230 17 38812795 38812860 66 - 1.636 1.601 -0.116
ENSG00000273559 E018 27.3575252 0.0007250776 6.020240e-02 0.1269497313 17 38812861 38812864 4 - 1.498 1.370 -0.440
ENSG00000273559 E019 74.1914861 0.0003889373 2.955736e-01 0.4367008019 17 38814861 38815097 237 - 1.889 1.846 -0.146
ENSG00000273559 E020 1.0997650 0.0147324757 2.089142e-02 0.0533599881 17 38815625 38815741 117 - 0.097 0.495 3.087
ENSG00000273559 E021 68.5699143 0.0004665681 2.785888e-01 0.4182640044 17 38820901 38821073 173 - 1.857 1.810 -0.158
ENSG00000273559 E022 49.9876954 0.0086372782 4.461605e-01 0.5876806779 17 38825166 38825269 104 - 1.726 1.672 -0.182
ENSG00000273559 E023 25.2066813 0.0286514152 9.565981e-01 0.9767784825 17 38825270 38825355 86 - 1.422 1.405 -0.057