ENSG00000270885

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000603017 ENSG00000270885 HEK293_OSMI2_2hA HEK293_TMG_2hB RASL10B protein_coding protein_coding 14.03835 26.80703 8.626122 1.443942 0.1127513 -1.634694 11.490659 19.682923 7.7321600 2.411461 0.1399378 -1.346869 0.8307292 0.7287333 0.8970 0.1682667 0.04008892 0.04008892 FALSE TRUE
MSTRG.14163.2 ENSG00000270885 HEK293_OSMI2_2hA HEK293_TMG_2hB RASL10B protein_coding   14.03835 26.80703 8.626122 1.443942 0.1127513 -1.634694 1.900491 5.863728 0.5437366 1.136904 0.2242999 -3.407005 0.1216875 0.2247000 0.0624 -0.1623000 0.05284813 0.04008892 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000270885 E001 11.398292 0.0072346391 7.111592e-02 1.451924e-01 17 35731639 35731697 59 + 1.118 0.985 -0.484
ENSG00000270885 E002 48.666683 0.0143087602 1.057875e-01 1.992932e-01 17 35731698 35731878 181 + 1.656 1.610 -0.156
ENSG00000270885 E003 163.176922 0.0010533571 1.140715e-02 3.213411e-02 17 35735038 35735336 299 + 2.148 2.138 -0.033
ENSG00000270885 E004 111.159899 0.0003286808 1.563439e-03 5.840723e-03 17 35735337 35735400 64 + 2.012 1.967 -0.150
ENSG00000270885 E005 5.920728 0.0043537274 1.740378e-01 2.934309e-01 17 35736776 35737072 297 + 0.561 0.834 1.145
ENSG00000270885 E006 139.500140 0.0003402136 2.330314e-05 1.422906e-04 17 35740409 35740533 125 + 2.123 2.056 -0.223
ENSG00000270885 E007 106.544535 0.0020596435 4.009581e-06 2.915942e-05 17 35741035 35741131 97 + 2.044 1.926 -0.397
ENSG00000270885 E008 300.459590 0.0033440785 1.003182e-02 2.884001e-02 17 35741132 35741804 673 + 2.410 2.407 -0.012
ENSG00000270885 E009 1034.500502 0.0064266908 4.637648e-07 4.129481e-06 17 35741805 35743521 1717 + 2.795 2.985 0.631