ENSG00000263528

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000578328 ENSG00000263528 HEK293_OSMI2_2hA HEK293_TMG_2hB IKBKE protein_coding protein_coding 0.8459797 2.017339 0.1136196 0.1336163 0.01415013 -4.035608 0.11764919 0.2651742 0.06663324 0.04576302 0.03351658 -1.844303 0.2150208 0.1301000 0.6666667 0.5365667 0.28898388 0.01229415 FALSE TRUE
ENST00000579827 ENSG00000263528 HEK293_OSMI2_2hA HEK293_TMG_2hB IKBKE protein_coding protein_coding 0.8459797 2.017339 0.1136196 0.1336163 0.01415013 -4.035608 0.36465843 0.8125560 0.00000000 0.09679728 0.00000000 -6.362042 0.2194750 0.3999667 0.0000000 -0.3999667 0.01229415 0.01229415 FALSE TRUE
ENST00000581977 ENSG00000263528 HEK293_OSMI2_2hA HEK293_TMG_2hB IKBKE protein_coding protein_coding 0.8459797 2.017339 0.1136196 0.1336163 0.01415013 -4.035608 0.28264162 0.6394739 0.01889495 0.11295107 0.01889495 -4.490382 0.3674667 0.3278000 0.1340333 -0.1937667 0.58796890 0.01229415 FALSE TRUE
ENST00000584998 ENSG00000263528 HEK293_OSMI2_2hA HEK293_TMG_2hB IKBKE protein_coding protein_coding 0.8459797 2.017339 0.1136196 0.1336163 0.01415013 -4.035608 0.07267719 0.3001346 0.02809142 0.11689316 0.02809142 -3.025356 0.1508833 0.1422000 0.1993000 0.0571000 0.90918998 0.01229415 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000263528 E001 2.792790 0.021694808 0.38926217 0.53337162 1 206470476 206470494 19 + 0.515 0.346 -0.902
ENSG00000263528 E002 9.791283 0.002116181 0.53203590 0.66440746 1 206470495 206470641 147 + 0.825 0.752 -0.289
ENSG00000263528 E003 8.065790 0.002953670 0.13670431 0.24348901 1 206470642 206470698 57 + 0.330 0.715 1.879
ENSG00000263528 E004 8.978655 0.002041366 0.42964188 0.57234073 1 206471156 206471245 90 + 0.825 0.723 -0.410
ENSG00000263528 E005 0.000000       1 206472854 206472963 110 +      
ENSG00000263528 E006 8.728152 0.002285234 0.38301744 0.52716794 1 206473196 206473314 119 + 0.825 0.707 -0.474
ENSG00000263528 E007 12.317515 0.001493939 0.58276321 0.70704363 1 206474331 206474471 141 + 0.894 0.842 -0.197
ENSG00000263528 E008 9.137302 0.003294803 0.10343780 0.19572103 1 206474865 206474994 130 + 0.330 0.746 2.005
ENSG00000263528 E009 3.014059 0.005330304 0.24642755 0.38176776 1 206474995 206475135 141 + 0.000 0.412 11.896
ENSG00000263528 E010 6.937948 0.006680873 0.24583919 0.38105970 1 206476181 206476362 182 + 0.330 0.640 1.564
ENSG00000263528 E011 6.593454 0.003517269 0.26327814 0.40116049 1 206476678 206476838 161 + 0.330 0.629 1.519
ENSG00000263528 E012 4.939126 0.006958243 0.47019293 0.60939605 1 206477749 206477859 111 + 0.330 0.534 1.090
ENSG00000263528 E013 0.000000       1 206477860 206478159 300 +      
ENSG00000263528 E014 5.977178 0.005082052 0.30315115 0.44504688 1 206478160 206478339 180 + 0.330 0.609 1.431
ENSG00000263528 E015 6.243690 0.073019232 0.41281363 0.55639485 1 206478943 206479133 191 + 0.745 0.597 -0.626
ENSG00000263528 E016 3.504108 0.046377067 0.01892509 0.04916829 1 206479870 206479934 65 + 0.826 0.394 -1.945
ENSG00000263528 E017 4.374952 0.006323152 0.03616955 0.08391459 1 206480022 206480113 92 + 0.825 0.470 -1.543
ENSG00000263528 E018 3.523788 0.093107560 0.07792363 0.15614645 1 206480447 206480533 87 + 0.745 0.399 -1.599
ENSG00000263528 E019 3.657231 0.046213934 0.63774429 0.75156637 1 206484997 206485072 76 + 0.515 0.442 -0.364
ENSG00000263528 E020 4.534506 0.098112437 0.15905000 0.27385095 1 206485194 206485306 113 + 0.744 0.485 -1.144
ENSG00000263528 E021 4.182636 0.004543787 0.56995430 0.69647049 1 206487914 206487990 77 + 0.330 0.497 0.911
ENSG00000263528 E022 3.301196 0.005531255 0.77518406 0.85513815 1 206490819 206490858 40 + 0.330 0.427 0.557
ENSG00000263528 E023 3.452609 0.005292383 0.18294488 0.30487648 1 206491648 206491749 102 + 0.000 0.456 12.121
ENSG00000263528 E024 4.739188 0.005449045 0.08719239 0.17088582 1 206493023 206493119 97 + 0.000 0.556 12.596
ENSG00000263528 E025 3.053800 0.005351834 0.24611613 0.38138997 1 206493266 206493378 113 + 0.000 0.412 11.896
ENSG00000263528 E026 2.826025 0.005746056 0.27384988 0.41294914 1 206493920 206493991 72 + 0.000 0.396 11.811
ENSG00000263528 E027 21.025568 0.033421056 0.43648438 0.57855574 1 206496112 206496889 778 + 0.893 1.063 0.632