ENSG00000261341

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000562076 ENSG00000261341 HEK293_OSMI2_2hA HEK293_TMG_2hB protein_coding protein_coding 2.261581 4.512347 2.320541 0.5484039 0.464651 -0.9564067 0.1825615 0.3638303 0.0000000 0.3638303 0.0000000 -5.224311569 0.06857083 0.0740 0.0000000 -0.0740000 0.896682622 0.004225627   FALSE
ENST00000563228 ENSG00000261341 HEK293_OSMI2_2hA HEK293_TMG_2hB protein_coding nonsense_mediated_decay 2.261581 4.512347 2.320541 0.5484039 0.464651 -0.9564067 0.3687628 0.3257563 0.6534414 0.1158255 0.1231756 0.982554548 0.20923750 0.0738 0.2878333 0.2140333 0.004225627 0.004225627   FALSE
ENST00000636757 ENSG00000261341 HEK293_OSMI2_2hA HEK293_TMG_2hB protein_coding protein_coding 2.261581 4.512347 2.320541 0.5484039 0.464651 -0.9564067 0.4878524 0.5725065 0.5731651 0.1650350 0.3019355 0.001630369 0.22700417 0.1405 0.3290333 0.1885333 0.894972794 0.004225627   FALSE
MSTRG.17562.4 ENSG00000261341 HEK293_OSMI2_2hA HEK293_TMG_2hB protein_coding   2.261581 4.512347 2.320541 0.5484039 0.464651 -0.9564067 1.2224042 3.2502542 1.0939349 0.6186731 0.6870276 -1.562329433 0.49518333 0.7117 0.3831333 -0.3285667 0.564020851 0.004225627   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000261341 E001 4.2877767 0.004067930 8.525576e-05 0.0004528528 19 50776141 50776466 326 - 0.975 0.454 -2.195
ENSG00000261341 E002 2.9527391 0.005746056 3.570527e-01 0.5014743079 19 50785728 50785730 3 - 0.479 0.619 0.654
ENSG00000261341 E003 3.2095846 0.005746056 2.046151e-01 0.3319322918 19 50785731 50785742 12 - 0.479 0.671 0.875
ENSG00000261341 E004 3.5396839 0.005528567 5.211545e-01 0.6550449526 19 50785743 50785750 8 - 0.581 0.671 0.391
ENSG00000261341 E005 5.5897895 0.004573706 2.267314e-01 0.3584902091 19 50785751 50785823 73 - 0.701 0.849 0.592
ENSG00000261341 E006 5.2961837 0.003615506 1.867966e-01 0.3097070991 19 50785942 50786001 60 - 0.664 0.832 0.681
ENSG00000261341 E007 0.3729606 0.028605254 7.659552e-01 0.8485957580 19 50786090 50786160 71 - 0.147 0.102 -0.607
ENSG00000261341 E008 6.6988647 0.003448741 4.724335e-02 0.1042613954 19 50789618 50789715 98 - 0.701 0.937 0.927
ENSG00000261341 E009 2.0785733 0.010893634 6.374700e-01 0.7513595866 19 50792405 50792504 100 - 0.533 0.454 -0.390
ENSG00000261341 E010 0.9275265 0.017325392 1.862706e-01 0.3090676221 19 50793114 50793142 29 - 0.416 0.184 -1.611
ENSG00000261341 E011 8.8329284 0.003416954 1.014483e-01 0.1926724739 19 50795820 50795868 49 - 1.074 0.909 -0.609