ENSG00000259495

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000559008 ENSG00000259495 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA lncRNA 0.6745631 0.7611059 0.6668824 0.06968688 0.116768 -0.1880239 0.19440805 0.12613232 0.3364822 0.03010098 0.02150385 1.3477719 0.33539583 0.16476667 0.5324667 0.36770000 0.02440911 0.02440911   FALSE
ENST00000559267 ENSG00000259495 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA lncRNA 0.6745631 0.7611059 0.6668824 0.06968688 0.116768 -0.1880239 0.03325912 0.05767219 0.0000000 0.05767219 0.00000000 -2.7585630 0.03633333 0.09163333 0.0000000 -0.09163333 0.76917223 0.02440911   FALSE
ENST00000653750 ENSG00000259495 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA lncRNA 0.6745631 0.7611059 0.6668824 0.06968688 0.116768 -0.1880239 0.16723239 0.20511660 0.1162775 0.09173120 0.02147100 -0.7685207 0.24560000 0.27233333 0.1925333 -0.07980000 0.81047767 0.02440911   FALSE
ENST00000656160 ENSG00000259495 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA lncRNA 0.6745631 0.7611059 0.6668824 0.06968688 0.116768 -0.1880239 0.27966354 0.37218483 0.2141226 0.11648309 0.13570058 -0.7699825 0.38266667 0.47123333 0.2749667 -0.19626667 0.65085035 0.02440911   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000259495 E001 0.0000000       15 80344853 80344868 16 -      
ENSG00000259495 E002 0.4782907 0.0219373644 4.741377e-02 1.045690e-01 15 80344869 80344903 35 - 0.000 0.337 11.668
ENSG00000259495 E003 2.6172420 0.0084543374 8.724967e-03 2.559807e-02 15 80344904 80345127 224 - 0.319 0.755 2.114
ENSG00000259495 E004 0.1482932 0.0428200367 3.649906e-01   15 80347688 80347698 11 - 0.000 0.143 11.744
ENSG00000259495 E005 2.1398962 0.1776250438 3.182934e-01 4.612627e-01 15 80347699 80347784 86 - 0.372 0.640 1.316
ENSG00000259495 E006 39.0895621 0.0008414589 1.088720e-12 2.653135e-11 15 80373413 80380153 6741 - 1.682 1.482 -0.682
ENSG00000259495 E007 1.5049169 0.0138882286 4.073047e-03 1.334809e-02 15 80397944 80398006 63 - 0.104 0.616 3.530
ENSG00000259495 E008 0.0000000       15 80398007 80398007 1 -      
ENSG00000259495 E009 3.1312081 0.0112983314 2.400971e-03 8.464025e-03 15 80402659 80402960 302 - 0.319 0.812 2.340
ENSG00000259495 E010 3.3839021 0.0048231129 1.249234e-02 3.471669e-02 15 80403502 80403585 84 - 0.419 0.811 1.754
ENSG00000259495 E011 5.6440031 0.0889181768 1.690238e-01 2.869241e-01 15 80403709 80404214 506 - 0.679 0.934 1.007