ENSG00000259248

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000558831 ENSG00000259248 HEK293_OSMI2_2hA HEK293_TMG_2hB USP3-AS1 lncRNA lncRNA 0.3104738 0.376496 0.3782186 0.05749599 0.1199176 0.006415637 0.027446763 0.04897621 0.07179165 0.03383296 0.03807365 0.4718203 0.08568750 0.1158000 0.19503333 0.07923333 0.82782153 0.02486396   FALSE
ENST00000559861 ENSG00000259248 HEK293_OSMI2_2hA HEK293_TMG_2hB USP3-AS1 lncRNA lncRNA 0.3104738 0.376496 0.3782186 0.05749599 0.1199176 0.006415637 0.022086693 0.00000000 0.11928412 0.00000000 0.02311906 3.6924731 0.06591250 0.0000000 0.41250000 0.41250000 0.02486396 0.02486396 TRUE FALSE
ENST00000560622 ENSG00000259248 HEK293_OSMI2_2hA HEK293_TMG_2hB USP3-AS1 lncRNA lncRNA 0.3104738 0.376496 0.3782186 0.05749599 0.1199176 0.006415637 0.092721704 0.05745900 0.14153898 0.05745900 0.14153898 1.1676062 0.21587500 0.1275000 0.23003333 0.10253333 1.00000000 0.02486396   FALSE
ENST00000560962 ENSG00000259248 HEK293_OSMI2_2hA HEK293_TMG_2hB USP3-AS1 lncRNA lncRNA 0.3104738 0.376496 0.3782186 0.05749599 0.1199176 0.006415637 0.029572871 0.00000000 0.01025510 0.00000000 0.01025510 1.0182852 0.12051250 0.0000000 0.01666667 0.01666667 0.86821842 0.02486396   FALSE
ENST00000561191 ENSG00000259248 HEK293_OSMI2_2hA HEK293_TMG_2hB USP3-AS1 lncRNA lncRNA 0.3104738 0.376496 0.3782186 0.05749599 0.1199176 0.006415637 0.008576993 0.06861594 0.00000000 0.06861594 0.00000000 -2.9748219 0.02064583 0.1651667 0.00000000 -0.16516667 0.76771929 0.02486396   FALSE
ENST00000657335 ENSG00000259248 HEK293_OSMI2_2hA HEK293_TMG_2hB USP3-AS1 lncRNA lncRNA 0.3104738 0.376496 0.3782186 0.05749599 0.1199176 0.006415637 0.025248959 0.09881433 0.03175456 0.05796305 0.02347731 -1.3818628 0.12322083 0.3309000 0.13346667 -0.19743333 0.80333543 0.02486396   FALSE
ENST00000665700 ENSG00000259248 HEK293_OSMI2_2hA HEK293_TMG_2hB USP3-AS1 lncRNA lncRNA 0.3104738 0.376496 0.3782186 0.05749599 0.1199176 0.006415637 0.039995675 0.03638242 0.00000000 0.01877194 0.00000000 -2.2135782 0.15473750 0.1136667 0.00000000 -0.11366667 0.27566909 0.02486396   FALSE
ENST00000668026 ENSG00000259248 HEK293_OSMI2_2hA HEK293_TMG_2hB USP3-AS1 lncRNA lncRNA 0.3104738 0.376496 0.3782186 0.05749599 0.1199176 0.006415637 0.028329735 0.04784515 0.00000000 0.04784515 0.00000000 -2.5321960 0.11575000 0.1061667 0.00000000 -0.10616667 0.68913257 0.02486396 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000259248 E001 0.0000000       15 63544247 63544288 42 -      
ENSG00000259248 E002 0.0000000       15 63544289 63544379 91 -      
ENSG00000259248 E003 0.0000000       15 63551295 63551342 48 -      
ENSG00000259248 E004 0.1472490 0.048018990 0.269896618   15 63551830 63551876 47 - 0.163 0.000 -12.107
ENSG00000259248 E005 0.0000000       15 63552912 63556248 3337 -      
ENSG00000259248 E006 0.0000000       15 63556249 63556290 42 -      
ENSG00000259248 E007 0.2987644 0.031498726 0.080546524   15 63556291 63556608 318 - 0.282 0.000 -15.077
ENSG00000259248 E008 0.0000000       15 63556642 63556831 190 -      
ENSG00000259248 E009 0.7427016 0.016832902 0.002197925 0.007835643 15 63556832 63556893 62 - 0.516 0.000 -16.077
ENSG00000259248 E010 0.7394793 0.017035472 0.039135047 0.089435418 15 63556894 63556979 86 - 0.451 0.090 -2.979
ENSG00000259248 E011 0.1472490 0.048018990 0.269896618   15 63587132 63587709 578 - 0.163 0.000 -14.280
ENSG00000259248 E012 0.8512514 0.015122100 0.458395904 0.598722664 15 63587710 63587883 174 - 0.163 0.285 1.021
ENSG00000259248 E013 4.0880829 0.011468334 0.124372699 0.226212183 15 63587884 63588828 945 - 0.812 0.579 -0.973
ENSG00000259248 E014 1.3725078 0.011323708 0.050920897 0.110805197 15 63588829 63588962 134 - 0.573 0.229 -1.980
ENSG00000259248 E015 1.4413167 0.014026607 0.399262223 0.542995697 15 63588963 63589100 138 - 0.282 0.418 0.825
ENSG00000259248 E016 0.1482932 0.046088416 0.838592498   15 63590921 63591070 150 - 0.000 0.090 11.670
ENSG00000259248 E017 0.4448795 0.034528210 0.230509504 0.363066986 15 63591071 63591185 115 - 0.000 0.228 13.014
ENSG00000259248 E018 0.9200498 0.019264321 0.320503541 0.463644609 15 63591186 63591335 150 - 0.163 0.333 1.338
ENSG00000259248 E019 0.3299976 0.028752000 0.417493743   15 63593749 63593877 129 - 0.000 0.165 12.529
ENSG00000259248 E020 3.1720102 0.008019727 0.339141966 0.483067510 15 63594695 63594786 92 - 0.708 0.550 -0.691
ENSG00000259248 E021 0.8126288 0.333367398 0.195830513 0.321013267 15 63599183 63599303 121 - 0.000 0.293 13.323
ENSG00000259248 E022 4.7320035 0.065414414 0.008825807 0.025846696 15 63599304 63600261 958 - 0.375 0.818 2.023
ENSG00000259248 E023 3.8601019 0.005183144 0.063692042 0.132847429 15 63600262 63600588 327 - 0.451 0.714 1.193
ENSG00000259248 E024 3.2409819 0.644098391 0.922947954 0.955405527 15 63600589 63600809 221 - 0.620 0.584 -0.159
ENSG00000259248 E025 0.0000000       15 63601258 63601589 332 -