ENSG00000257556

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000546968 ENSG00000257556 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA lncRNA 0.9065409 1.573137 0.3603543 0.2890083 0.0916409 -2.095808 0.12407819 0.07721995 0.06886797 0.077219947 0.03577995 -0.1452185 0.12395417 0.07276667 0.2230000 0.15023333 0.52025511 0.00114254   FALSE
ENST00000548104 ENSG00000257556 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA lncRNA 0.9065409 1.573137 0.3603543 0.2890083 0.0916409 -2.095808 0.27388785 0.65256674 0.00000000 0.243217734 0.00000000 -6.0499939 0.24525417 0.38143333 0.0000000 -0.38143333 0.00114254 0.00114254   FALSE
ENST00000548361 ENSG00000257556 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA lncRNA 0.9065409 1.573137 0.3603543 0.2890083 0.0916409 -2.095808 0.07743758 0.26540757 0.05589362 0.133065774 0.05589362 -2.0633574 0.08237083 0.14670000 0.1828667 0.03616667 0.89686713 0.00114254 TRUE FALSE
ENST00000655276 ENSG00000257556 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA lncRNA 0.9065409 1.573137 0.3603543 0.2890083 0.0916409 -2.095808 0.17092754 0.18035586 0.09596360 0.006789336 0.02487515 -0.8451302 0.26535833 0.12523333 0.2919667 0.16673333 0.24479610 0.00114254 TRUE FALSE
ENST00000659265 ENSG00000257556 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA lncRNA 0.9065409 1.573137 0.3603543 0.2890083 0.0916409 -2.095808 0.09111336 0.25064639 0.07807170 0.088827562 0.07807170 -1.5653434 0.09917917 0.15180000 0.1448000 -0.00700000 0.92920727 0.00114254 TRUE FALSE
ENST00000686753 ENSG00000257556 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA lncRNA 0.9065409 1.573137 0.3603543 0.2890083 0.0916409 -2.095808 0.10006295 0.03954653 0.00000000 0.039546530 0.00000000 -2.3087840 0.09000000 0.03726667 0.0000000 -0.03726667 1.00000000 0.00114254 FALSE FALSE
MSTRG.10276.2 ENSG00000257556 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA   0.9065409 1.573137 0.3603543 0.2890083 0.0916409 -2.095808 0.06903348 0.10739381 0.06155737 0.053798668 0.03078268 -0.7141842 0.09387917 0.08483333 0.1573667 0.07253333 0.71846637 0.00114254 TRUE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000257556 E001 0.3289534 0.030331637 0.38097735   14 105092739 105092909 171 + 0.199 0.073 -1.674
ENSG00000257556 E002 5.0443580 0.004915707 0.16324008 0.27938697 14 105093609 105093720 112 + 0.522 0.758 1.023
ENSG00000257556 E003 1.9572443 0.009662608 0.03041679 0.07277834 14 105093721 105093820 100 + 0.000 0.477 10.892
ENSG00000257556 E004 7.5770985 0.002539837 0.34581534 0.48995377 14 105093973 105095002 1030 + 0.932 0.834 -0.376
ENSG00000257556 E005 2.1305322 0.007973262 0.52863720 0.66154990 14 105095003 105095190 188 + 0.522 0.421 -0.506
ENSG00000257556 E006 3.6054334 0.009703967 0.71735758 0.81277244 14 105095191 105095326 136 + 0.522 0.593 0.329
ENSG00000257556 E007 9.3465217 0.002146932 0.58607535 0.70970768 14 105095327 105095442 116 + 0.869 0.946 0.292
ENSG00000257556 E008 2.4522110 0.045092511 0.70044286 0.79991419 14 105095443 105095539 97 + 0.522 0.452 -0.342
ENSG00000257556 E009 12.9779708 0.001822702 0.95751172 0.97736044 14 105095540 105095768 229 + 1.059 1.070 0.037
ENSG00000257556 E010 2.5829730 0.010827066 0.87153749 0.92136854 14 105098473 105098573 101 + 0.438 0.478 0.204
ENSG00000257556 E011 0.5169874 0.021768165 0.67115310 0.77745878 14 105098574 105098674 101 + 0.199 0.135 -0.674
ENSG00000257556 E012 2.5744679 0.007153640 0.36809140 0.51249237 14 105098719 105098890 172 + 0.592 0.451 -0.674
ENSG00000257556 E013 1.2930104 0.011783521 0.89924031 0.93971268 14 105098891 105099004 114 + 0.335 0.320 -0.092
ENSG00000257556 E014 2.1702992 0.040244973 0.54166992 0.67262535 14 105099044 105100131 1088 + 0.522 0.421 -0.508