ENSG00000256683

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000243644 ENSG00000256683 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF350 protein_coding protein_coding 2.394843 1.888771 2.620363 0.1695387 0.1339849 0.4701964 1.43051394 0.47859353 2.0407710 0.03847345 0.06001287 2.0694598 0.60875000 0.25400000 0.78306667 0.52906667 4.907420e-08 1.01519e-11 FALSE TRUE
ENST00000594929 ENSG00000256683 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF350 protein_coding protein_coding 2.394843 1.888771 2.620363 0.1695387 0.1339849 0.4701964 0.09379375 0.23254199 0.1166264 0.09304191 0.06396173 -0.9376563 0.05637083 0.12733333 0.04243333 -0.08490000 3.976941e-01 1.01519e-11   FALSE
ENST00000597788 ENSG00000256683 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF350 protein_coding protein_coding 2.394843 1.888771 2.620363 0.1695387 0.1339849 0.4701964 0.18433416 0.04965326 0.2981241 0.04965326 0.08352965 2.3688385 0.07742917 0.02833333 0.11263333 0.08430000 2.762019e-01 1.01519e-11   FALSE
ENST00000599258 ENSG00000256683 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF350 protein_coding processed_transcript 2.394843 1.888771 2.620363 0.1695387 0.1339849 0.4701964 0.34573847 1.01921822 0.0000000 0.25490963 0.00000000 -6.6854051 0.14130833 0.52593333 0.00000000 -0.52593333 1.015190e-11 1.01519e-11   FALSE
ENST00000600703 ENSG00000256683 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF350 protein_coding processed_transcript 2.394843 1.888771 2.620363 0.1695387 0.1339849 0.4701964 0.22675513 0.10876354 0.0000000 0.10876354 0.00000000 -3.5700201 0.07427083 0.06443333 0.00000000 -0.06443333 7.330178e-01 1.01519e-11 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000256683 E001 43.7256084 0.0007377223 1.431948e-01 2.523729e-01 19 51964340 51965855 1516 - 1.631 1.574 -0.192
ENSG00000256683 E002 15.0161405 0.0015074989 1.057132e-01 1.991770e-01 19 51965856 51966214 359 - 1.202 1.061 -0.505
ENSG00000256683 E003 0.2214452 0.0396675213 1.646186e-01   19 51967288 51967400 113 - 0.000 0.192 11.782
ENSG00000256683 E004 0.1817044 0.0420123398 1.649639e-01   19 51968479 51968577 99 - 0.000 0.191 11.770
ENSG00000256683 E005 3.8952192 0.0098486423 2.317821e-01 3.645713e-01 19 51968578 51968613 36 - 0.588 0.777 0.796
ENSG00000256683 E006 3.7760722 0.0058340975 7.471904e-01 8.347528e-01 19 51968614 51968629 16 - 0.629 0.688 0.252
ENSG00000256683 E007 5.0585764 0.0032296521 8.050258e-01 8.759390e-01 19 51968630 51968658 29 - 0.732 0.777 0.179
ENSG00000256683 E008 4.7032185 0.0243049722 3.369364e-01 4.807972e-01 19 51968659 51968673 15 - 0.666 0.817 0.615
ENSG00000256683 E009 7.6530340 0.0355238727 4.538316e-01 5.945442e-01 19 51969005 51969131 127 - 0.927 0.818 -0.419
ENSG00000256683 E010 0.0000000       19 51969132 51969145 14 -      
ENSG00000256683 E011 1.3403442 0.0123640266 3.833724e-02 8.798260e-02 19 51972967 51974345 1379 - 0.435 0.000 -12.892
ENSG00000256683 E012 12.0137484 0.0019961093 3.846414e-01 5.288000e-01 19 51974346 51974531 186 - 1.096 1.018 -0.286
ENSG00000256683 E013 1.5123699 0.0094729061 6.453185e-01 7.574388e-01 19 51974678 51974853 176 - 0.403 0.324 -0.468
ENSG00000256683 E014 2.1087597 0.0093683215 1.791192e-06 1.407975e-05 19 51981925 51982031 107 - 0.076 0.850 4.991
ENSG00000256683 E015 1.8014901 0.0107392820 6.800604e-06 4.697736e-05 19 51984048 51984169 122 - 0.076 0.814 4.851
ENSG00000256683 E016 0.2924217 0.0278374165 6.752063e-01   19 51986510 51986769 260 - 0.141 0.000 -10.928
ENSG00000256683 E017 7.8797521 0.0024123997 6.057410e-01 7.260728e-01 19 51986770 51986856 87 - 0.897 0.968 0.268