ENSG00000256340

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000565118 ENSG00000256340 HEK293_OSMI2_2hA HEK293_TMG_2hB ABCC6P1 transcribed_unprocessed_pseudogene processed_transcript 1.017837 1.825549 0.7671875 0.1423481 0.08200301 -1.239877 0.91413656 1.75659511 0.5909625 0.13595111 0.06976750 -1.555624 0.8531250 0.9623333 0.7688 -0.1935333 0.0012688971 0.0006313473 FALSE FALSE
ENST00000565647 ENSG00000256340 HEK293_OSMI2_2hA HEK293_TMG_2hB ABCC6P1 transcribed_unprocessed_pseudogene processed_transcript 1.017837 1.825549 0.7671875 0.1423481 0.08200301 -1.239877 0.07664751 0.04326373 0.1762250 0.02252356 0.01470715 1.805821 0.1157333 0.0253000 0.2312 0.2059000 0.0006313473 0.0006313473 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000256340 E001 0.0000000       16 18571162 18571187 26 +      
ENSG00000256340 E002 0.0000000       16 18571188 18571215 28 +      
ENSG00000256340 E003 1.4350481 0.011584967 0.4196125916 0.562871533 16 18571216 18571275 60 + 0.265 0.401 0.852
ENSG00000256340 E004 2.1045951 0.008781422 0.3184606901 0.461432851 16 18571276 18571304 29 + 0.354 0.515 0.851
ENSG00000256340 E005 1.5896840 0.010208080 0.2950630574 0.436178906 16 18571305 18571311 7 + 0.265 0.442 1.073
ENSG00000256340 E006 5.9334785 0.003025251 0.3471167585 0.491297935 16 18572877 18573060 184 + 0.750 0.848 0.391
ENSG00000256340 E007 9.5450918 0.002728370 0.0041231474 0.013486850 16 18573061 18573539 479 + 0.810 1.073 0.990
ENSG00000256340 E008 0.1817044 0.043220597 0.7398247088   16 18574320 18574435 116 + 0.000 0.098 9.009
ENSG00000256340 E009 0.4804688 0.023560932 0.3250475362 0.468479445 16 18574761 18574886 126 + 0.265 0.098 -1.735
ENSG00000256340 E010 0.3289534 0.031050982 0.7282082629   16 18575026 18575154 129 + 0.152 0.098 -0.734
ENSG00000256340 E011 0.1817044 0.043220597 0.7398247088   16 18580210 18580335 126 + 0.000 0.098 9.009
ENSG00000256340 E012 0.1451727 0.048157837 0.3081860771   16 18582406 18582467 62 + 0.152 0.000 -10.897
ENSG00000256340 E013 0.5202097 0.022761724 0.3249805502 0.468407845 16 18585806 18585937 132 + 0.265 0.098 -1.733
ENSG00000256340 E014 0.9598804 0.016595388 0.0229565387 0.057653676 16 18591104 18591307 204 + 0.491 0.098 -3.055
ENSG00000256340 E015 0.8909948 0.014209446 0.0225113376 0.056744709 16 18592539 18592716 178 + 0.491 0.098 -3.058
ENSG00000256340 E016 3.6944005 0.004903654 0.0005694386 0.002419342 16 18596767 18598328 1562 + 0.910 0.442 -2.016