ENSG00000256294

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000262894 ENSG00000256294 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF225 protein_coding protein_coding 1.646841 0.6330514 2.386728 0.07621739 0.2413286 1.89806 0.8638040 0.49821482 1.32523666 0.04991038 0.06026056 1.3935851 0.5807833 0.80696667 0.5675333 -0.23943333 3.384569e-01 1.132596e-05 FALSE TRUE
ENST00000588926 ENSG00000256294 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF225 protein_coding protein_coding 1.646841 0.6330514 2.386728 0.07621739 0.2413286 1.89806 0.1999773 0.06190218 0.28797851 0.03117776 0.16746260 2.0511008 0.1172458 0.08746667 0.1098667 0.02240000 9.936610e-01 1.132596e-05   FALSE
ENST00000589155 ENSG00000256294 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF225 protein_coding protein_coding 1.646841 0.6330514 2.386728 0.07621739 0.2413286 1.89806 0.4011478 0.00000000 0.68061232 0.00000000 0.07574069 6.1098042 0.1741667 0.00000000 0.2871000 0.28710000 1.132596e-05 1.132596e-05 FALSE TRUE
ENST00000590612 ENSG00000256294 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF225 protein_coding protein_coding 1.646841 0.6330514 2.386728 0.07621739 0.2413286 1.89806 0.1426522 0.03676697 0.06382351 0.03676697 0.04208547 0.6585903 0.0907625 0.05096667 0.0240000 -0.02696667 8.404724e-01 1.132596e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000256294 E001 0.0000000       19 44112181 44112297 117 +      
ENSG00000256294 E002 0.3030308 0.3092865385 7.697709e-01   19 44113399 44113414 16 + 0.145 0.000 -9.368
ENSG00000256294 E003 0.3030308 0.3092865385 7.697709e-01   19 44113415 44113416 2 + 0.145 0.000 -11.014
ENSG00000256294 E004 0.4545463 0.6428667928 7.038464e-01 0.8024649600 19 44113417 44113430 14 + 0.204 0.000 -11.275
ENSG00000256294 E005 10.0920600 0.0019121334 7.771892e-01 0.8565969645 19 44113431 44113569 139 + 1.004 1.028 0.088
ENSG00000256294 E006 2.1024101 0.0102643237 6.565439e-01 0.7660630890 19 44114049 44114213 165 + 0.501 0.416 -0.434
ENSG00000256294 E007 1.1155605 0.0124892801 3.590553e-01 0.5034001254 19 44115545 44115759 215 + 0.253 0.416 1.025
ENSG00000256294 E008 9.0030048 0.0020651248 4.561818e-01 0.5966411667 19 44115760 44115842 83 + 0.995 0.902 -0.350
ENSG00000256294 E009 8.9537004 0.0024093778 4.070622e-01 0.5506780103 19 44118188 44118314 127 + 1.004 0.901 -0.383
ENSG00000256294 E010 6.6203830 0.0046262259 3.723240e-02 0.0859185178 19 44118482 44118574 93 + 0.930 0.625 -1.223
ENSG00000256294 E011 0.2998086 0.0288883373 4.576460e-01   19 44128454 44129041 588 + 0.078 0.186 1.439
ENSG00000256294 E012 0.0000000       19 44129042 44129098 57 +      
ENSG00000256294 E013 0.8178402 0.0242970362 1.460075e-02 0.0395970894 19 44129099 44130700 1602 + 0.078 0.497 3.438
ENSG00000256294 E014 9.8256561 0.0018262802 2.754360e-02 0.0670968279 19 44130850 44130968 119 + 1.074 0.804 -1.018
ENSG00000256294 E015 50.5557602 0.0043353890 7.188742e-02 0.1464779693 19 44130969 44132209 1241 + 1.705 1.604 -0.343
ENSG00000256294 E016 59.2675026 0.0004493799 3.424531e-05 0.0002007573 19 44132210 44134822 2613 + 1.697 1.846 0.504