ENSG00000255121

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000526453 ENSG00000255121 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA lncRNA 12.56651 19.79421 9.489903 1.707234 0.2921741 -1.059822 1.585360 2.328804 1.870626 0.3497711 0.06583838 -0.3145573 0.1377542 0.1163667 0.1979000 0.08153333 0.007908978 0.007908978    
ENST00000582695 ENSG00000255121 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA lncRNA 12.56651 19.79421 9.489903 1.707234 0.2921741 -1.059822 1.995819 1.979188 1.367134 0.5011658 0.16577914 -0.5305107 0.1725833 0.0982000 0.1435333 0.04533333 0.371381023 0.007908978    
MSTRG.6414.1 ENSG00000255121 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA   12.56651 19.79421 9.489903 1.707234 0.2921741 -1.059822 4.386899 7.519479 3.482902 0.4224663 0.55073638 -1.1081238 0.3344875 0.3828667 0.3675667 -0.01530000 0.936343161 0.007908978    
MSTRG.6414.2 ENSG00000255121 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA   12.56651 19.79421 9.489903 1.707234 0.2921741 -1.059822 4.598432 7.966737 2.769241 0.8670089 0.45477654 -1.5211080 0.3551750 0.4025667 0.2910333 -0.11153333 0.239285238 0.007908978    
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000255121 E001 212.80874 0.0022938439 1.024217e-04 5.332531e-04 11 118991718 118992218 501 - 2.190 2.288 0.329
ENSG00000255121 E002 56.36540 0.0015706651 3.700194e-05 2.150014e-04 11 118992219 118992342 124 - 1.513 1.742 0.779
ENSG00000255121 E003 23.20070 0.0147426149 1.646530e-01 2.812310e-01 11 118994824 118994970 147 - 1.422 1.290 -0.461
ENSG00000255121 E004 14.51073 0.0013693465 6.155148e-02 1.292479e-01 11 118996195 118996198 4 - 1.251 1.073 -0.635
ENSG00000255121 E005 107.55041 0.0041716152 1.500327e-07 1.472783e-06 11 118996199 118997276 1078 - 2.117 1.906 -0.707
ENSG00000255121 E006 94.15132 0.0004495376 7.827178e-01 8.605126e-01 11 118997728 118998004 277 - 1.927 1.910 -0.056