ENSG00000253982

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000518481 ENSG00000253982 HEK293_OSMI2_2hA HEK293_TMG_2hB CLN8-AS1 lncRNA lncRNA 9.458693 17.66393 7.342167 2.078837 0.1022618 -1.265382 7.6219833 14.9818570 4.8065405 2.0320427 0.2875865 -1.6381099 0.76293750 0.84470000 0.6540333 -0.19066667 0.000240299 0.000240299   FALSE
ENST00000659375 ENSG00000253982 HEK293_OSMI2_2hA HEK293_TMG_2hB CLN8-AS1 lncRNA lncRNA 9.458693 17.66393 7.342167 2.078837 0.1022618 -1.265382 0.4479886 0.3709955 0.4765357 0.2227378 0.4765357 0.3527718 0.05007917 0.02316667 0.0632000 0.04003333 0.823658718 0.000240299   FALSE
ENST00000665209 ENSG00000253982 HEK293_OSMI2_2hA HEK293_TMG_2hB CLN8-AS1 lncRNA lncRNA 9.458693 17.66393 7.342167 2.078837 0.1022618 -1.265382 0.7253948 1.0768956 1.0218327 0.1350821 0.5169536 -0.0750043 0.11444583 0.06100000 0.1410000 0.08000000 0.932296333 0.000240299   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000253982 E001 0.0000000       8 1761054 1761063 10 -      
ENSG00000253982 E002 0.1515154 0.0424015081 2.366678e-01   8 1761064 1761257 194 - 0.166 0.000 -9.802
ENSG00000253982 E003 8.4992427 0.0042014042 1.218253e-02 3.396732e-02 8 1761258 1761359 102 - 1.101 0.827 -1.024
ENSG00000253982 E004 38.4918411 0.0006750752 2.915141e-06 2.186927e-05 8 1761360 1761824 465 - 1.713 1.466 -0.841
ENSG00000253982 E005 7.4239556 0.0295377044 5.940192e-03 1.845141e-02 8 1761825 1761843 19 - 1.116 0.759 -1.348
ENSG00000253982 E006 14.9137069 0.0100675444 5.716379e-02 1.217442e-01 8 1761844 1761989 146 - 1.281 1.108 -0.616
ENSG00000253982 E007 12.2294772 0.0290389072 3.235855e-01 4.668910e-01 8 1761990 1762080 91 - 1.161 1.055 -0.380
ENSG00000253982 E008 37.1287483 0.0089856387 6.720976e-04 2.796487e-03 8 1762081 1762624 544 - 1.689 1.465 -0.763
ENSG00000253982 E009 63.6610125 0.0004543007 2.524489e-01 3.887982e-01 8 1762625 1762778 154 - 1.717 1.777 0.204
ENSG00000253982 E010 123.3818151 0.0002870439 2.901505e-03 9.973534e-03 8 1762779 1762928 150 - 1.973 2.072 0.334
ENSG00000253982 E011 109.2183534 0.0077780220 1.134377e-04 5.836462e-04 8 1763023 1763346 324 - 1.843 2.043 0.670
ENSG00000253982 E012 0.6997360 0.0167852359 1.568966e-01 2.709608e-01 8 1764301 1764433 133 - 0.000 0.281 11.488
ENSG00000253982 E013 0.6997360 0.0167852359 1.568966e-01 2.709608e-01 8 1764434 1764508 75 - 0.000 0.281 11.488