ENSG00000253771

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000429698 ENSG00000253771 HEK293_OSMI2_2hA HEK293_TMG_2hB   transcribed_unprocessed_pseudogene processed_transcript 0.8694164 1.236217 0.6280253 0.2697042 0.07885643 -0.9658692 0.09532499 0.06197988 0.00000000 0.06197988 0.00000000 -2.8475938 0.10913333 0.05816667 0.00000000 -0.05816667 0.8767240481 0.0004889347 TRUE TRUE
ENST00000649209 ENSG00000253771 HEK293_OSMI2_2hA HEK293_TMG_2hB   transcribed_unprocessed_pseudogene processed_transcript 0.8694164 1.236217 0.6280253 0.2697042 0.07885643 -0.9658692 0.08783057 0.05120654 0.09194258 0.01933766 0.02517110 0.7359990 0.11708333 0.04233333 0.16230000 0.11996667 0.1774548664 0.0004889347 TRUE FALSE
ENST00000687945 ENSG00000253771 HEK293_OSMI2_2hA HEK293_TMG_2hB   transcribed_unprocessed_pseudogene processed_transcript 0.8694164 1.236217 0.6280253 0.2697042 0.07885643 -0.9658692 0.46750228 0.71847162 0.40504536 0.16912482 0.09698637 -0.8116038 0.53471667 0.59250000 0.62486667 0.03236667 0.9077934050 0.0004889347 FALSE TRUE
ENST00000688349 ENSG00000253771 HEK293_OSMI2_2hA HEK293_TMG_2hB   transcribed_unprocessed_pseudogene processed_transcript 0.8694164 1.236217 0.6280253 0.2697042 0.07885643 -0.9658692 0.01895863 0.00000000 0.06056846 0.00000000 0.03903022 2.8190236 0.02687500 0.00000000 0.09360000 0.09360000 0.1202618890 0.0004889347   FALSE
ENST00000688382 ENSG00000253771 HEK293_OSMI2_2hA HEK293_TMG_2hB   transcribed_unprocessed_pseudogene processed_transcript 0.8694164 1.236217 0.6280253 0.2697042 0.07885643 -0.9658692 0.01063072 0.00000000 0.04825283 0.00000000 0.04825283 2.5423283 0.01684167 0.00000000 0.07266667 0.07266667 0.6564777530 0.0004889347   FALSE
ENST00000692136 ENSG00000253771 HEK293_OSMI2_2hA HEK293_TMG_2hB   transcribed_unprocessed_pseudogene processed_transcript 0.8694164 1.236217 0.6280253 0.2697042 0.07885643 -0.9658692 0.07863021 0.26783306 0.00000000 0.06705607 0.00000000 -4.7961464 0.07972500 0.21763333 0.00000000 -0.21763333 0.0004889347 0.0004889347 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000253771 E001 0.0000000       13 24924673 24924673 1 -      
ENSG00000253771 E002 0.0000000       13 24924674 24924674 1 -      
ENSG00000253771 E003 0.0000000       13 24924675 24924676 2 -      
ENSG00000253771 E004 1.3232125 0.182658018 0.207907138 0.335978091 13 24924677 24924875 199 - 0.159 0.423 1.892
ENSG00000253771 E005 1.6156342 0.064345530 0.720816717 0.815360429 13 24925848 24925894 47 - 0.367 0.425 0.323
ENSG00000253771 E006 1.5521082 0.017261466 0.595788050 0.717830669 13 24933006 24933494 489 - 0.443 0.342 -0.564
ENSG00000253771 E007 0.7425998 0.017842445 0.267325435 0.405635532 13 24933495 24933536 42 - 0.367 0.169 -1.477
ENSG00000253771 E008 2.6834917 0.019387492 0.001652486 0.006129629 13 24933537 24934663 1127 - 0.830 0.340 -2.276
ENSG00000253771 E009 1.7359165 0.017100133 0.207683152 0.335696308 13 24937577 24937770 194 - 0.563 0.341 -1.154
ENSG00000253771 E010 0.8867284 0.014691467 0.016491335 0.043823679 13 24940477 24940506 30 - 0.507 0.093 -3.212
ENSG00000253771 E011 2.2079412 0.007481982 0.006035035 0.018702161 13 24940507 24940785 279 - 0.735 0.292 -2.210
ENSG00000253771 E012 2.6924718 0.008016681 0.996810141 1.000000000 13 24941802 24941986 185 - 0.563 0.559 -0.018
ENSG00000253771 E013 6.8638957 0.003363570 0.102447525 0.194248510 13 24941987 24942108 122 - 0.735 0.925 0.742
ENSG00000253771 E014 3.1331852 0.005746056 0.704235393 0.802816652 13 24942932 24943021 90 - 0.563 0.613 0.225
ENSG00000253771 E015 0.3299976 0.029078516 0.410464373   13 24951203 24951387 185 - 0.000 0.170 11.328
ENSG00000253771 E016 0.4031496 0.030552079 0.403296968 0.547071085 13 24951388 24951396 9 - 0.000 0.170 11.331
ENSG00000253771 E017 7.9248754 0.002342247 0.002429270 0.008549885 13 24951397 24951488 92 - 0.657 1.014 1.395
ENSG00000253771 E018 0.0000000       13 24951489 24951490 2 -      
ENSG00000253771 E019 0.0000000       13 24951595 24951770 176 -      
ENSG00000253771 E020 0.0000000       13 24953281 24953344 64 -      
ENSG00000253771 E021 0.0000000       13 24954773 24954776 4 -      
ENSG00000253771 E022 0.1515154 0.047049400 0.282700136   13 24954777 24954869 93 - 0.159 0.000 -12.571
ENSG00000253771 E023 0.0000000       13 24959734 24959812 79 -      
ENSG00000253771 E024 0.7426990 0.079762187 0.654636093 0.764755449 13 24961749 24961849 101 - 0.159 0.236 0.705
ENSG00000253771 E025 0.1482932 0.046213934 0.798293217   13 24961850 24961854 5 - 0.000 0.093 10.494
ENSG00000253771 E026 0.1451727 0.051488127 0.282573636   13 24965500 24965580 81 - 0.159 0.000 -12.567
ENSG00000253771 E027 0.0000000       13 24967242 24967391 150 -      
ENSG00000253771 E028 0.6664265 0.019200607 0.556344107 0.685019089 13 24967392 24967453 62 - 0.275 0.170 -0.888
ENSG00000253771 E029 0.0000000       13 24967454 24967454 1 -      
ENSG00000253771 E030 0.1515154 0.047049400 0.282700136   13 24967739 24968205 467 - 0.159 0.000 -12.571
ENSG00000253771 E031 7.5844868 0.003038917 0.428679110 0.571400226 13 24968206 24968505 300 - 0.857 0.937 0.302