ENSG00000253738

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000524003 ENSG00000253738 HEK293_OSMI2_2hA HEK293_TMG_2hB OTUD6B-AS1 lncRNA lncRNA 8.641024 4.333714 13.56885 0.1574095 0.6760094 1.64436 6.2575710 2.47759436 10.337194 0.18703939 0.6170295 2.0564165 0.68887500 0.5731667 0.7611333 0.1879667 5.301062e-03 2.626923e-05 TRUE FALSE
ENST00000657110 ENSG00000253738 HEK293_OSMI2_2hA HEK293_TMG_2hB OTUD6B-AS1 lncRNA lncRNA 8.641024 4.333714 13.56885 0.1574095 0.6760094 1.64436 0.5501284 0.08949015 1.001970 0.08949015 0.1841190 3.3464685 0.06271667 0.0222000 0.0751000 0.0529000 2.354051e-01 2.626923e-05 TRUE FALSE
MSTRG.31797.2 ENSG00000253738 HEK293_OSMI2_2hA HEK293_TMG_2hB OTUD6B-AS1 lncRNA   8.641024 4.333714 13.56885 0.1574095 0.6760094 1.64436 1.5552453 1.59603303 1.799513 0.25949796 0.1688382 0.1720999 0.21615417 0.3660667 0.1321333 -0.2339333 2.626923e-05 2.626923e-05 TRUE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000253738 E001 5.768707 0.0031235381 8.315128e-02 1.644991e-01 8 91059318 91060669 1352 - 0.739 0.934 0.758
ENSG00000253738 E002 2.373188 0.0143745795 9.942623e-01 1.000000e+00 8 91060670 91060727 58 - 0.524 0.500 -0.114
ENSG00000253738 E003 8.304297 0.0092409022 9.652751e-01 9.821500e-01 8 91060728 91062909 2182 - 0.945 0.906 -0.146
ENSG00000253738 E004 23.700789 0.0057564418 3.609918e-02 8.377800e-02 8 91062910 91063617 708 - 1.316 1.448 0.458
ENSG00000253738 E005 37.166363 0.0006632006 1.386103e-15 5.144850e-14 8 91063618 91063746 129 - 1.381 1.794 1.412
ENSG00000253738 E006 6.151528 0.0026324830 5.550484e-02 1.188457e-01 8 91065071 91067910 2840 - 0.894 0.570 -1.331
ENSG00000253738 E007 175.310037 0.0004325425 5.600638e-01 6.880993e-01 8 91067911 91068935 1025 - 2.216 2.192 -0.078
ENSG00000253738 E008 179.252207 0.0132208476 2.792345e-05 1.672679e-04 8 91068936 91069578 643 - 2.282 2.020 -0.877
ENSG00000253738 E009 160.772264 0.0005821695 3.710432e-02 8.567095e-02 8 91069579 91069822 244 - 2.168 2.189 0.068
ENSG00000253738 E010 87.778186 0.0003149181 7.527303e-01 8.388431e-01 8 91069931 91070583 653 - 1.928 1.877 -0.171