ENSG00000251369

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000344222 ENSG00000251369 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF550 protein_coding protein_coding 2.699648 1.346156 3.477982 0.0788097 0.115356 1.362869 0.9051225 0.3471264 1.0063561 0.06206148 0.10887656 1.5088993 0.3288292 0.2642333 0.2912000 0.02696667 0.8939449722 0.0002010187 TRUE TRUE
ENST00000447310 ENSG00000251369 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF550 protein_coding nonsense_mediated_decay 2.699648 1.346156 3.477982 0.0788097 0.115356 1.362869 0.7489404 0.3493814 0.7017067 0.11087865 0.06460460 0.9857669 0.3025667 0.2524000 0.2018333 -0.05056667 0.7378095958 0.0002010187 TRUE TRUE
ENST00000457177 ENSG00000251369 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF550 protein_coding protein_coding 2.699648 1.346156 3.477982 0.0788097 0.115356 1.362869 0.8606791 0.6496480 1.2240126 0.03055414 0.07942692 0.9035889 0.3168917 0.4833333 0.3517667 -0.13156667 0.1952393668 0.0002010187 TRUE TRUE
MSTRG.17798.6 ENSG00000251369 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF550 protein_coding   2.699648 1.346156 3.477982 0.0788097 0.115356 1.362869 0.1014730 0.0000000 0.5459061 0.00000000 0.14163873 5.7967694 0.0266625 0.0000000 0.1552667 0.15526667 0.0002010187 0.0002010187 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000251369 E001 0.0000000       19 57535257 57535391 135 -      
ENSG00000251369 E002 0.1817044 0.0403650822 2.461884e-01   19 57541836 57541839 4 - 0.000 0.166 9.879
ENSG00000251369 E003 32.3713415 0.0114788358 1.031942e-06 8.535183e-06 19 57541840 57542635 796 - 1.327 1.703 1.290
ENSG00000251369 E004 35.1657099 0.0064099327 9.290051e-02 1.796715e-01 19 57542636 57543101 466 - 1.498 1.621 0.421
ENSG00000251369 E005 20.0595953 0.0010574483 9.232927e-01 9.556371e-01 19 57543102 57543243 142 - 1.308 1.313 0.017
ENSG00000251369 E006 11.3516949 0.0064171145 2.127016e-01 3.417350e-01 19 57543244 57543650 407 - 1.023 1.161 0.498
ENSG00000251369 E007 23.4910558 0.0040187795 6.904226e-01 7.921180e-01 19 57543651 57544236 586 - 1.359 1.401 0.148
ENSG00000251369 E008 15.9134815 0.0018402127 5.517220e-01 6.811430e-01 19 57544237 57544583 347 - 1.228 1.189 -0.140
ENSG00000251369 E009 24.6120329 0.0009006728 7.946566e-02 1.586825e-01 19 57546457 57546762 306 - 1.426 1.313 -0.393
ENSG00000251369 E010 60.6906273 0.0004815989 2.311366e-03 8.186229e-03 19 57546763 57547831 1069 - 1.814 1.694 -0.404
ENSG00000251369 E011 17.5216586 0.0012434172 5.671528e-02 1.209739e-01 19 57547832 57547993 162 - 1.298 1.148 -0.533
ENSG00000251369 E012 13.8104054 0.0591934392 3.037562e-01 4.457210e-01 19 57552627 57552722 96 - 1.191 1.064 -0.456
ENSG00000251369 E013 0.0000000       19 57555998 57556230 233 -      
ENSG00000251369 E014 14.5310726 0.0013387403 2.799902e-01 4.197859e-01 19 57556231 57556357 127 - 1.204 1.118 -0.309
ENSG00000251369 E015 0.2214452 0.0373741722 2.461203e-01   19 57556358 57556360 3 - 0.000 0.166 11.794
ENSG00000251369 E016 10.3789458 0.0448051484 3.829350e-01 5.270890e-01 19 57559656 57559863 208 - 1.084 0.971 -0.413