ENSG00000251322

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000445220 ENSG00000251322 HEK293_OSMI2_2hA HEK293_TMG_2hB SHANK3 protein_coding protein_coding 9.079987 9.850703 8.920715 0.9309127 0.306169 -0.1429148 2.5445402 4.152657 1.9754395 0.1206861 0.1844765 -1.0680465 0.2667875 0.42973333 0.22063333 -0.20910000 1.880321e-03 4.206262e-05 FALSE TRUE
MSTRG.22385.16 ENSG00000251322 HEK293_OSMI2_2hA HEK293_TMG_2hB SHANK3 protein_coding   9.079987 9.850703 8.920715 0.9309127 0.306169 -0.1429148 2.7004244 1.939931 3.2445661 0.2257069 0.1215364 0.7390424 0.3044333 0.19650000 0.36456667 0.16806667 4.206262e-05 4.206262e-05 FALSE TRUE
MSTRG.22385.5 ENSG00000251322 HEK293_OSMI2_2hA HEK293_TMG_2hB SHANK3 protein_coding   9.079987 9.850703 8.920715 0.9309127 0.306169 -0.1429148 1.9950467 2.373618 2.0200216 0.2496701 0.2818142 -0.2316581 0.2245542 0.24040000 0.22800000 -0.01240000 9.063879e-01 4.206262e-05 FALSE TRUE
MSTRG.22385.9 ENSG00000251322 HEK293_OSMI2_2hA HEK293_TMG_2hB SHANK3 protein_coding   9.079987 9.850703 8.920715 0.9309127 0.306169 -0.1429148 0.7161981 0.769190 0.1847719 0.4020676 0.1847719 -2.0001900 0.0746500 0.07116667 0.01973333 -0.05143333 5.668043e-01 4.206262e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000251322 E001 0.6256415 0.0175378760 1.724290e-01 2.913657e-01 22 50674415 50674704 290 + 0.323 0.110 -1.933
ENSG00000251322 E002 0.0000000       22 50674881 50674891 11 +      
ENSG00000251322 E003 2.9067362 0.0056495801 8.801940e-04 3.544783e-03 22 50674892 50675013 122 + 0.813 0.333 -2.255
ENSG00000251322 E004 1.7305929 0.0087236148 1.366770e-01 2.434472e-01 22 50675014 50675021 8 + 0.553 0.333 -1.156
ENSG00000251322 E005 2.5034834 0.0065465709 2.754767e-02 6.710588e-02 22 50675022 50675047 26 + 0.702 0.388 -1.486
ENSG00000251322 E006 2.3144172 0.0070930505 4.859390e-02 1.066729e-01 22 50675048 50675051 4 + 0.669 0.388 -1.349
ENSG00000251322 E007 3.7098262 0.0047643923 5.922799e-02 1.253162e-01 22 50675052 50675064 13 + 0.788 0.556 -0.985
ENSG00000251322 E008 10.4184489 0.0016884008 7.241786e-02 1.473630e-01 22 50675065 50675162 98 + 1.118 0.985 -0.485
ENSG00000251322 E009 14.8921647 0.0012500414 4.480898e-02 9.985212e-02 22 50675163 50675251 89 + 1.253 1.128 -0.444
ENSG00000251322 E010 15.9959716 0.0027677423 2.674514e-02 6.546254e-02 22 50676622 50676693 72 + 1.295 1.155 -0.495
ENSG00000251322 E011 20.1325185 0.0150525087 1.084453e-02 3.079325e-02 22 50678585 50678693 109 + 1.408 1.224 -0.642
ENSG00000251322 E012 26.1589924 0.0031301066 8.201789e-02 1.627020e-01 22 50678769 50678920 152 + 1.455 1.383 -0.250
ENSG00000251322 E013 32.7859461 0.0014382296 2.474551e-02 6.135351e-02 22 50679019 50679186 168 + 1.559 1.472 -0.300
ENSG00000251322 E014 26.1884443 0.0126116854 9.222850e-02 1.786052e-01 22 50679312 50679428 117 + 1.472 1.385 -0.301
ENSG00000251322 E015 20.7585006 0.0070572241 1.442591e-02 3.919108e-02 22 50683340 50683417 78 + 1.408 1.257 -0.526
ENSG00000251322 E016 22.9239465 0.0008422918 7.160943e-04 2.956563e-03 22 50684585 50684651 67 + 1.472 1.283 -0.656
ENSG00000251322 E017 0.5953508 0.0418734245 7.207619e-01 8.153179e-01 22 50688766 50694068 5303 + 0.239 0.197 -0.355
ENSG00000251322 E018 27.0921099 0.0036675622 3.826747e-02 8.785120e-02 22 50694775 50695048 274 + 1.488 1.393 -0.326
ENSG00000251322 E019 0.6695470 0.0202955955 5.112131e-01 6.462973e-01 22 50696023 50696044 22 + 0.136 0.271 1.235
ENSG00000251322 E020 1.6178123 0.0673155205 5.868928e-01 7.103298e-01 22 50697564 50697564 1 + 0.453 0.388 -0.349
ENSG00000251322 E021 2.3133836 0.0266820890 5.173013e-01 6.517163e-01 22 50697565 50697581 17 + 0.553 0.480 -0.345
ENSG00000251322 E022 1.2104824 0.0116455676 9.211407e-01 9.542284e-01 22 50697582 50697584 3 + 0.322 0.333 0.069
ENSG00000251322 E023 22.0390260 0.0030422961 1.805209e-01 3.018352e-01 22 50697585 50697715 131 + 1.383 1.332 -0.175
ENSG00000251322 E024 0.0000000       22 50697999 50698122 124 +      
ENSG00000251322 E025 0.0000000       22 50698123 50698143 21 +      
ENSG00000251322 E026 0.7749669 0.0184257432 7.914853e-02 1.581543e-01 22 50698144 50698342 199 + 0.392 0.110 -2.348
ENSG00000251322 E027 0.1515154 0.0421842074 3.453632e-01   22 50698343 50698689 347 + 0.136 0.000 -9.368
ENSG00000251322 E028 55.2189661 0.0011596467 9.859197e-04 3.910712e-03 22 50698690 50698803 114 + 1.800 1.692 -0.364
ENSG00000251322 E029 69.3394098 0.0004194982 3.908109e-04 1.739246e-03 22 50703860 50703935 76 + 1.889 1.792 -0.326
ENSG00000251322 E030 75.9459999 0.0004087137 9.501656e-04 3.788404e-03 22 50704166 50704248 83 + 1.918 1.838 -0.271
ENSG00000251322 E031 90.2011286 0.0003054219 2.185486e-03 7.796954e-03 22 50704738 50704862 125 + 1.979 1.919 -0.203
ENSG00000251322 E032 77.5076582 0.0003531721 6.631621e-04 2.763800e-03 22 50704964 50705096 133 + 1.928 1.845 -0.279
ENSG00000251322 E033 3.9158443 0.0040968249 3.670859e-02 8.491818e-02 22 50705376 50705495 120 + 0.813 0.556 -1.084
ENSG00000251322 E034 5.7391810 0.0029727784 1.189253e-01 2.184032e-01 22 50705496 50706071 576 + 0.902 0.750 -0.595
ENSG00000251322 E035 48.9763137 0.0059405830 7.198873e-02 1.466441e-01 22 50706072 50706152 81 + 1.710 1.657 -0.180
ENSG00000251322 E036 0.3686942 0.0279082146 7.916683e-01 8.667463e-01 22 50711615 50711638 24 + 0.136 0.110 -0.345
ENSG00000251322 E037 64.8644149 0.0003906734 4.350116e-03 1.412319e-02 22 50714917 50715047 131 + 1.843 1.771 -0.243
ENSG00000251322 E038 47.2153301 0.0008552498 1.739773e-01 2.933512e-01 22 50715669 50715753 85 + 1.681 1.663 -0.062
ENSG00000251322 E039 422.9937165 0.0035159797 5.476555e-01 6.776816e-01 22 50720184 50722437 2254 + 2.586 2.632 0.152
ENSG00000251322 E040 68.4985290 0.0039999252 1.232383e-07 1.230220e-06 22 50723398 50723551 154 + 1.589 1.936 1.173
ENSG00000251322 E041 8.3278094 0.0365889890 1.464216e-01 2.568129e-01 22 50724127 50724153 27 + 1.038 0.887 -0.563
ENSG00000251322 E042 25.7593826 0.0007466533 4.223001e-01 5.654436e-01 22 50729572 50729707 136 + 1.341 1.449 0.375
ENSG00000251322 E043 23.4686449 0.0008399754 2.162091e-03 7.726876e-03 22 50730721 50730802 82 + 1.175 1.462 1.005
ENSG00000251322 E044 13.4367335 0.0028697095 8.255670e-01 8.901807e-01 22 50730803 50730946 144 + 1.093 1.164 0.256
ENSG00000251322 E045 10.4141690 0.0134182866 8.791890e-02 1.719628e-01 22 50730947 50730982 36 + 1.118 0.975 -0.523
ENSG00000251322 E046 95.7988667 0.0032201718 9.544635e-01 9.754601e-01 22 50730983 50731710 728 + 1.937 1.991 0.181
ENSG00000251322 E047 403.2765399 0.0090159860 8.180537e-09 1.024986e-07 22 50731711 50733027 1317 + 2.388 2.692 1.013
ENSG00000251322 E048 68.7010963 0.0200939616 5.879676e-05 3.254790e-04 22 50733028 50733212 185 + 1.555 1.950 1.338