ENSG00000251247

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000420450 ENSG00000251247 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF345 protein_coding protein_coding 3.368028 3.394463 4.83286 0.7121369 0.2236843 0.5084321 0.3053337 0.0000000 0.3674018 0.00000000 0.1550243 5.2380295 0.09695417 0.0000000 0.07903333 0.079033333 0.005391046 0.005391046 FALSE TRUE
ENST00000432005 ENSG00000251247 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF345 protein_coding processed_transcript 3.368028 3.394463 4.83286 0.7121369 0.2236843 0.5084321 0.3466957 0.5014520 0.1997267 0.08846463 0.1000731 -1.2860885 0.13107917 0.1507000 0.04266667 -0.108033333 0.187872667 0.005391046   FALSE
ENST00000526123 ENSG00000251247 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF345 protein_coding protein_coding 3.368028 3.394463 4.83286 0.7121369 0.2236843 0.5084321 0.4500682 0.7647818 0.4175262 0.14618572 0.2309622 -0.8577772 0.15250417 0.2316333 0.09010000 -0.141533333 0.420855338 0.005391046   FALSE
ENST00000529555 ENSG00000251247 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF345 protein_coding protein_coding 3.368028 3.394463 4.83286 0.7121369 0.2236843 0.5084321 0.2623026 0.1733760 0.2795783 0.05028897 0.1215785 0.6591486 0.08615000 0.0535000 0.05803333 0.004533333 0.985586684 0.005391046 FALSE TRUE
MSTRG.17043.6 ENSG00000251247 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF345 protein_coding   3.368028 3.394463 4.83286 0.7121369 0.2236843 0.5084321 1.6549030 1.8085507 3.1207137 0.57027836 0.4120587 0.7837024 0.41889167 0.5132000 0.64043333 0.127233333 0.446638971 0.005391046   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000251247 E001 1.2910239 0.0103581362 4.267428e-01 5.695554e-01 19 36850361 36850515 155 + 0.406 0.261 -0.905
ENSG00000251247 E002 1.8037700 0.0082157629 2.824514e-01 4.224607e-01 19 36850876 36850877 2 + 0.359 0.540 0.939
ENSG00000251247 E003 1.8037700 0.0082157629 2.824514e-01 4.224607e-01 19 36850878 36850881 4 + 0.359 0.540 0.939
ENSG00000251247 E004 3.7632967 0.0043385078 6.180525e-01 7.357882e-01 19 36850882 36850894 13 + 0.638 0.709 0.298
ENSG00000251247 E005 11.8191921 0.0018458845 5.722947e-03 1.788009e-02 19 36850895 36850929 35 + 0.966 1.221 0.926
ENSG00000251247 E006 13.8903785 0.0014316242 5.136495e-03 1.630027e-02 19 36850930 36850968 39 + 1.043 1.281 0.851
ENSG00000251247 E007 4.1525924 0.0066177601 4.263772e-01 5.692090e-01 19 36851163 36851650 488 + 0.751 0.633 -0.492
ENSG00000251247 E008 1.3629308 0.0957236485 3.671728e-01 5.115611e-01 19 36851651 36851654 4 + 0.447 0.260 -1.137
ENSG00000251247 E009 1.2156818 0.0269755756 4.348576e-01 5.771178e-01 19 36851655 36851658 4 + 0.406 0.260 -0.910
ENSG00000251247 E010 1.5060272 0.0099880129 2.145598e-01 3.439299e-01 19 36851659 36851723 65 + 0.486 0.261 -1.324
ENSG00000251247 E011 1.3608545 0.0110861209 3.045735e-01 4.465945e-01 19 36851724 36851727 4 + 0.447 0.261 -1.132
ENSG00000251247 E012 1.3576322 0.0914526492 7.901922e-01 8.658008e-01 19 36851728 36851744 17 + 0.405 0.347 -0.335
ENSG00000251247 E013 1.8317583 0.1175729591 7.889497e-01 8.648946e-01 19 36851745 36851829 85 + 0.485 0.420 -0.334
ENSG00000251247 E014 14.2347591 0.0016096434 2.347842e-03 8.300363e-03 19 36851830 36851851 22 + 1.043 1.300 0.916
ENSG00000251247 E015 15.3952889 0.0013316400 6.312472e-02 1.319212e-01 19 36851852 36851904 53 + 1.142 1.290 0.524
ENSG00000251247 E016 1.1167064 0.0136283625 5.896525e-01 7.126918e-01 19 36854450 36854594 145 + 0.359 0.261 -0.646
ENSG00000251247 E017 4.4841161 0.0254664134 2.549435e-01 3.917231e-01 19 36862917 36863011 95 + 0.807 0.631 -0.722
ENSG00000251247 E018 0.0000000       19 36872551 36872697 147 +      
ENSG00000251247 E019 0.0000000       19 36872698 36872748 51 +      
ENSG00000251247 E020 5.3218679 0.0042807957 5.019322e-02 1.095010e-01 19 36876785 36876876 92 + 0.900 0.632 -1.080
ENSG00000251247 E021 42.1169517 0.0007926983 5.988069e-10 9.258318e-09 19 36876877 36879575 2699 + 1.737 1.430 -1.047
ENSG00000251247 E022 6.1025991 0.0030389172 1.874397e-03 6.836451e-03 19 36892818 36892985 168 + 0.638 1.020 1.500
ENSG00000251247 E023 1.2168397 0.0251874177 9.589881e-01 9.782923e-01 19 36892986 36893034 49 + 0.359 0.348 -0.064
ENSG00000251247 E024 0.8836053 0.0159385635 3.321334e-01 4.757639e-01 19 36912727 36913029 303 + 0.180 0.349 1.263