ENSG00000250565

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000505245 ENSG00000250565 HEK293_OSMI2_2hA HEK293_TMG_2hB ATP6V1E2 protein_coding processed_transcript 4.87632 4.852472 4.749902 0.3897089 0.1127239 -0.03075791 0.6915190 0.4371527 0.86086921 0.03741124 0.03481304 0.9616886 0.1446333 0.09003333 0.18170000 0.09166667 0.01400839 0.01400839    
ENST00000522587 ENSG00000250565 HEK293_OSMI2_2hA HEK293_TMG_2hB ATP6V1E2 protein_coding protein_coding 4.87632 4.852472 4.749902 0.3897089 0.1127239 -0.03075791 1.3449104 1.7364225 1.25271411 0.22299371 0.10788572 -0.4678746 0.2732042 0.35803333 0.26390000 -0.09413333 0.32985331 0.01400839 FALSE  
ENST00000524188 ENSG00000250565 HEK293_OSMI2_2hA HEK293_TMG_2hB ATP6V1E2 protein_coding processed_transcript 4.87632 4.852472 4.749902 0.3897089 0.1127239 -0.03075791 1.1359491 1.0594187 1.35836680 0.22561670 0.23322481 0.3556282 0.2274917 0.21453333 0.28423333 0.06970000 0.61179797 0.01400839    
MSTRG.18326.2 ENSG00000250565 HEK293_OSMI2_2hA HEK293_TMG_2hB ATP6V1E2 protein_coding   4.87632 4.852472 4.749902 0.3897089 0.1127239 -0.03075791 0.6331856 0.6133350 0.67999935 0.09823860 0.05663648 0.1465873 0.1338917 0.12526667 0.14276667 0.01750000 0.84687169 0.01400839 FALSE  
MSTRG.18326.5 ENSG00000250565 HEK293_OSMI2_2hA HEK293_TMG_2hB ATP6V1E2 protein_coding   4.87632 4.852472 4.749902 0.3897089 0.1127239 -0.03075791 0.5611605 0.5633097 0.38518937 0.19339156 0.24328696 -0.5367705 0.1185125 0.11070000 0.08336667 -0.02733333 0.77853478 0.01400839    
MSTRG.18326.6 ENSG00000250565 HEK293_OSMI2_2hA HEK293_TMG_2hB ATP6V1E2 protein_coding   4.87632 4.852472 4.749902 0.3897089 0.1127239 -0.03075791 0.2759871 0.2829224 0.05374844 0.28292244 0.05374844 -2.2000568 0.0549875 0.06943333 0.01080000 -0.05863333 1.00000000 0.01400839    
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000250565 E001 0.3299976 0.0274424043 2.406808e-01   2 46490750 46490831 82 - 0.000 0.207 9.254
ENSG00000250565 E002 47.2500125 0.0018042537 7.022872e-03 2.128365e-02 2 46511847 46512459 613 - 1.600 1.744 0.487
ENSG00000250565 E003 28.0965753 0.0165677305 5.578156e-03 1.749640e-02 2 46512460 46512610 151 - 1.303 1.552 0.861
ENSG00000250565 E004 30.0645119 0.0008021709 7.300195e-03 2.200127e-02 2 46512611 46512812 202 - 1.384 1.557 0.595
ENSG00000250565 E005 13.4963059 0.0014533584 5.032613e-01 6.392227e-01 2 46518945 46519643 699 - 1.192 1.140 -0.184
ENSG00000250565 E006 0.8470867 0.0147310890 5.497931e-01 6.794908e-01 2 46519644 46519957 314 - 0.310 0.207 -0.766
ENSG00000250565 E007 1.9656805 0.0100122825 9.965384e-01 1.000000e+00 2 46527878 46527940 63 - 0.489 0.496 0.034
ENSG00000250565 E008 17.3510977 0.0010889435 6.088704e-01 7.284701e-01 2 46527941 46528047 107 - 1.238 1.286 0.168
ENSG00000250565 E009 19.1228841 0.0011944328 2.712481e-06 2.050564e-05 2 46534300 46535812 1513 - 1.469 1.121 -1.220
ENSG00000250565 E010 28.0172492 0.0007800768 4.990269e-01 6.353779e-01 2 46535813 46535927 115 - 1.484 1.450 -0.118
ENSG00000250565 E011 20.5600346 0.0009747277 2.723811e-01 4.112581e-01 2 46536611 46536703 93 - 1.372 1.300 -0.252
ENSG00000250565 E012 1.1437748 0.0117923562 1.866191e-01 3.094940e-01 2 46537376 46537576 201 - 0.437 0.207 -1.504
ENSG00000250565 E013 22.2563105 0.0026406627 2.167991e-01 3.465547e-01 2 46541390 46541453 64 - 1.409 1.325 -0.289
ENSG00000250565 E014 21.4602141 0.0027975477 1.920393e-01 3.163038e-01 2 46541454 46541675 222 - 1.397 1.306 -0.314
ENSG00000250565 E015 31.1999115 0.0082250390 6.666590e-01 7.740772e-01 2 46541676 46542178 503 - 1.517 1.487 -0.102
ENSG00000250565 E016 17.0474442 0.0012364702 1.805294e-01 3.018469e-01 2 46542217 46542577 361 - 1.310 1.211 -0.347