ENSG00000250305

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000400069 ENSG00000250305 HEK293_OSMI2_2hA HEK293_TMG_2hB TRMT9B protein_coding protein_coding 0.4736103 0.4892159 0.9304224 0.05721606 0.04921455 0.9136449 0.028364725 0.02242402 0.03936971 0.022424020 0.013728071 0.6065633 0.09111667 0.05976667 0.04126667 -0.01850000 9.963682e-01 4.96781e-06   FALSE
ENST00000447063 ENSG00000250305 HEK293_OSMI2_2hA HEK293_TMG_2hB TRMT9B protein_coding protein_coding 0.4736103 0.4892159 0.9304224 0.05721606 0.04921455 0.9136449 0.006627966 0.05302373 0.00000000 0.053023725 0.000000000 -2.6558950 0.01231667 0.09853333 0.00000000 -0.09853333 6.132793e-01 4.96781e-06 FALSE TRUE
ENST00000524591 ENSG00000250305 HEK293_OSMI2_2hA HEK293_TMG_2hB TRMT9B protein_coding protein_coding 0.4736103 0.4892159 0.9304224 0.05721606 0.04921455 0.9136449 0.064371408 0.02055435 0.11446052 0.006762926 0.027010567 2.0262387 0.16956250 0.04316667 0.12640000 0.08323333 4.145499e-01 4.96781e-06 FALSE TRUE
ENST00000525249 ENSG00000250305 HEK293_OSMI2_2hA HEK293_TMG_2hB TRMT9B protein_coding processed_transcript 0.4736103 0.4892159 0.9304224 0.05721606 0.04921455 0.9136449 0.012046349 0.07004558 0.00000000 0.035235945 0.000000000 -3.0008218 0.02836250 0.16023333 0.00000000 -0.16023333 1.237502e-01 4.96781e-06   FALSE
ENST00000528335 ENSG00000250305 HEK293_OSMI2_2hA HEK293_TMG_2hB TRMT9B protein_coding processed_transcript 0.4736103 0.4892159 0.9304224 0.05721606 0.04921455 0.9136449 0.075603272 0.14325534 0.00000000 0.143255341 0.000000000 -3.9378654 0.16630417 0.25840000 0.00000000 -0.25840000 6.756731e-01 4.96781e-06   FALSE
ENST00000528753 ENSG00000250305 HEK293_OSMI2_2hA HEK293_TMG_2hB TRMT9B protein_coding protein_coding 0.4736103 0.4892159 0.9304224 0.05721606 0.04921455 0.9136449 0.031205871 0.05910900 0.01768513 0.040282262 0.017685127 -1.3197624 0.07210000 0.10910000 0.01746667 -0.09163333 2.808360e-01 4.96781e-06   FALSE
ENST00000529706 ENSG00000250305 HEK293_OSMI2_2hA HEK293_TMG_2hB TRMT9B protein_coding retained_intron 0.4736103 0.4892159 0.9304224 0.05721606 0.04921455 0.9136449 0.037052183 0.01535186 0.06472800 0.015351861 0.002001505 1.5595573 0.09041250 0.02853333 0.06973333 0.04120000 4.657044e-01 4.96781e-06   FALSE
ENST00000529978 ENSG00000250305 HEK293_OSMI2_2hA HEK293_TMG_2hB TRMT9B protein_coding retained_intron 0.4736103 0.4892159 0.9304224 0.05721606 0.04921455 0.9136449 0.091231810 0.07721986 0.15983882 0.013038965 0.039332390 0.9614377 0.20451667 0.16703333 0.16903333 0.00200000 1.000000e+00 4.96781e-06 FALSE TRUE
ENST00000532376 ENSG00000250305 HEK293_OSMI2_2hA HEK293_TMG_2hB TRMT9B protein_coding protein_coding 0.4736103 0.4892159 0.9304224 0.05721606 0.04921455 0.9136449 0.127106721 0.02823219 0.53434018 0.028232188 0.014584209 3.8316488 0.16530833 0.07526667 0.57610000 0.50083333 4.967810e-06 4.96781e-06   TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000250305 E001 0.0000000       8 12945642 12945661 20 +      
ENSG00000250305 E002 0.0000000       8 12945673 12945673 1 +      
ENSG00000250305 E003 0.0000000       8 12945674 12945856 183 +      
ENSG00000250305 E004 0.0000000       8 12945857 12945966 110 +      
ENSG00000250305 E005 0.0000000       8 12951329 12951331 3 +      
ENSG00000250305 E006 0.9567719 0.017478212 0.93956051 0.96606198 8 12951332 12951381 50 + 0.277 0.290 0.090
ENSG00000250305 E007 1.3137493 0.014006374 0.01344743 0.03695833 8 12951635 12952021 387 + 0.160 0.585 2.673
ENSG00000250305 E008 1.2857253 0.011297488 0.73993750 0.82950146 8 12952022 12952062 41 + 0.325 0.385 0.353
ENSG00000250305 E009 5.1230067 0.003962395 0.58035203 0.70511169 8 12952063 12952358 296 + 0.755 0.826 0.281
ENSG00000250305 E010 0.5503986 0.021917621 0.22047949 0.35098934 8 12969997 12970150 154 + 0.087 0.290 2.092
ENSG00000250305 E011 2.1013885 0.007540756 0.10301660 0.19506433 8 12990834 12990939 106 + 0.369 0.636 1.311
ENSG00000250305 E012 2.2057762 0.008009554 0.39634520 0.54011616 8 12990940 12991000 61 + 0.445 0.585 0.674
ENSG00000250305 E013 0.6589510 0.018813637 0.08045695 0.16027685 8 12991001 12991031 31 + 0.087 0.384 2.673
ENSG00000250305 E014 0.8375349 0.019840259 0.20549371 0.33302964 8 13006202 13006233 32 + 0.160 0.385 1.676
ENSG00000250305 E015 1.7640291 0.009645602 0.12545653 0.22780074 8 13006234 13006356 123 + 0.325 0.585 1.351
ENSG00000250305 E016 1.5135132 0.009676551 0.43298116 0.57542604 8 13006357 13007670 1314 + 0.325 0.462 0.769
ENSG00000250305 E017 0.1515154 0.045262026 1.00000000   8 13007671 13007671 1 + 0.087 0.000 -8.849
ENSG00000250305 E018 1.7046834 0.011611416 0.72994233 0.82200934 8 13010262 13012012 1751 + 0.445 0.384 -0.327
ENSG00000250305 E019 0.7739347 0.015088193 0.70871717 0.80630528 8 13012013 13012683 671 + 0.223 0.290 0.503
ENSG00000250305 E020 0.9137968 0.016188496 0.40087131 0.54455273 8 13012684 13012692 9 + 0.325 0.169 -1.232
ENSG00000250305 E021 1.8255173 0.014124976 0.23192152 0.36474348 8 13012693 13012793 101 + 0.509 0.290 -1.231
ENSG00000250305 E022 0.8428203 0.016283938 0.70448273 0.80301646 8 13012794 13012857 64 + 0.223 0.290 0.507
ENSG00000250305 E023 3.6335286 0.006451638 0.53664227 0.66837116 8 13021008 13021233 226 + 0.680 0.586 -0.412
ENSG00000250305 E024 24.3683398 0.001350883 0.01843644 0.04809198 8 13021234 13026165 4932 + 1.429 1.291 -0.482
ENSG00000250305 E025 5.5321523 0.091544558 0.32398555 0.46733865 8 13026166 13029777 3612 + 0.861 0.678 -0.736
ENSG00000250305 E026 0.1817044 0.039170415 0.22772754   8 13031114 13031503 390 + 0.000 0.169 9.755