ENSG00000249348

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000504032 ENSG00000249348 HEK293_OSMI2_2hA HEK293_TMG_2hB UGDH-AS1 lncRNA lncRNA 2.717555 2.698074 2.563597 0.2902821 0.1502795 -0.07348095 1.0098957 0.5168764 1.416388 0.05778398 0.11942927 1.4368302 0.3788625 0.1963333 0.5535333 0.3572 2.569475e-05 2.569475e-05 FALSE FALSE
ENST00000685788 ENSG00000249348 HEK293_OSMI2_2hA HEK293_TMG_2hB UGDH-AS1 lncRNA lncRNA 2.717555 2.698074 2.563597 0.2902821 0.1502795 -0.07348095 1.4078515 1.6923742 0.896360 0.15812084 0.01972385 -0.9093921 0.5130583 0.6292667 0.3511667 -0.2781 2.915030e-05 2.569475e-05 FALSE FALSE
MSTRG.24750.1 ENSG00000249348 HEK293_OSMI2_2hA HEK293_TMG_2hB UGDH-AS1 lncRNA   2.717555 2.698074 2.563597 0.2902821 0.1502795 -0.07348095 0.2998081 0.4888235 0.250849 0.14564802 0.12792896 -0.9353144 0.1080958 0.1744000 0.0953000 -0.0791 6.435126e-01 2.569475e-05 TRUE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000249348 E001 6.4141100 0.003236661 8.103506e-01 8.796461e-01 4 39527720 39528018 299 + 0.866 0.814 -0.204
ENSG00000249348 E002 22.2154418 0.001004829 2.291893e-01 3.614684e-01 4 39528019 39528338 320 + 1.293 1.348 0.192
ENSG00000249348 E003 18.3863190 0.001178346 1.174869e-02 3.293363e-02 4 39531969 39532130 162 + 1.137 1.307 0.601
ENSG00000249348 E004 26.9313847 0.001278908 3.564267e-01 5.008045e-01 4 39532131 39532525 395 + 1.400 1.428 0.095
ENSG00000249348 E005 14.4273819 0.001765957 8.973632e-01 9.384434e-01 4 39534507 39534595 89 + 1.175 1.139 -0.129
ENSG00000249348 E006 0.5806908 0.860643513 2.019370e-01 3.286262e-01 4 39587923 39588059 137 + 0.428 0.000 -12.037
ENSG00000249348 E007 0.4355181 0.644628207 1.759787e-01 2.959673e-01 4 39589587 39589719 133 + 0.354 0.000 -11.625
ENSG00000249348 E008 4.8312585 0.011055878 1.386250e-09 2.001988e-08 4 39592673 39594707 2035 + 1.123 0.243 -4.033