ENSG00000249115

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000203166 ENSG00000249115 HEK293_OSMI2_2hA HEK293_TMG_2hB HAUS5 protein_coding protein_coding 20.20115 22.79509 17.09483 1.853625 0.126545 -0.4149522 7.593634 14.2497269 4.733873 1.28507332 0.6216821 -1.5878090 0.37247917 0.62573333 0.27750000 -0.34823333 8.587550e-06 1.042051e-31 FALSE TRUE
ENST00000430749 ENSG00000249115 HEK293_OSMI2_2hA HEK293_TMG_2hB HAUS5 protein_coding retained_intron 20.20115 22.79509 17.09483 1.853625 0.126545 -0.4149522 1.673697 1.0798118 1.188419 0.23523252 0.1721449 0.1370535 0.07942083 0.04686667 0.06956667 0.02270000 4.157454e-01 1.042051e-31   FALSE
ENST00000585968 ENSG00000249115 HEK293_OSMI2_2hA HEK293_TMG_2hB HAUS5 protein_coding retained_intron 20.20115 22.79509 17.09483 1.853625 0.126545 -0.4149522 6.316477 4.8789718 4.478168 0.88193401 0.4375913 -0.1234043 0.30798750 0.21443333 0.26160000 0.04716667 6.767506e-01 1.042051e-31 TRUE TRUE
ENST00000588570 ENSG00000249115 HEK293_OSMI2_2hA HEK293_TMG_2hB HAUS5 protein_coding retained_intron 20.20115 22.79509 17.09483 1.853625 0.126545 -0.4149522 1.178352 0.4090908 1.333188 0.10246738 0.1904414 1.6803267 0.06242083 0.01806667 0.07783333 0.05976667 5.607186e-05 1.042051e-31   FALSE
ENST00000592291 ENSG00000249115 HEK293_OSMI2_2hA HEK293_TMG_2hB HAUS5 protein_coding nonsense_mediated_decay 20.20115 22.79509 17.09483 1.853625 0.126545 -0.4149522 1.698018 0.5933671 3.634366 0.01453095 0.1424206 2.5945597 0.09121250 0.02626667 0.21256667 0.18630000 1.042051e-31 1.042051e-31 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000249115 E001 0.7362571 0.0889251377 2.395763e-01 3.736202e-01 19 35612735 35612743 9 + 0.381 0.161 -1.648
ENSG00000249115 E002 4.5799778 0.0042564206 1.378330e-01 2.450661e-01 19 35612744 35612751 8 + 0.850 0.643 -0.844
ENSG00000249115 E003 8.3693068 0.0020205999 3.774512e-01 5.217819e-01 19 35612752 35612762 11 + 0.996 0.891 -0.390
ENSG00000249115 E004 18.3940914 0.0011777654 5.550371e-02 1.188446e-01 19 35612763 35612770 8 + 1.344 1.184 -0.563
ENSG00000249115 E005 64.7140884 0.0074062364 3.413602e-01 4.852543e-01 19 35612771 35612799 29 + 1.804 1.750 -0.185
ENSG00000249115 E006 124.0202306 0.0085383040 3.907337e-01 5.346972e-01 19 35612800 35612892 93 + 2.077 2.036 -0.137
ENSG00000249115 E007 4.8714571 0.0034220617 2.574462e-03 8.987821e-03 19 35613669 35613729 61 + 0.974 0.570 -1.635
ENSG00000249115 E008 138.6007438 0.0056979613 1.298765e-01 2.339919e-01 19 35613730 35613792 63 + 2.142 2.075 -0.224
ENSG00000249115 E009 12.5109815 0.0014832977 7.031423e-05 3.816526e-04 19 35613793 35613867 75 + 1.315 0.939 -1.354
ENSG00000249115 E010 132.9236310 0.0002472944 2.463583e-03 8.654473e-03 19 35613868 35613900 33 + 2.145 2.045 -0.336
ENSG00000249115 E011 14.5693041 0.0012197151 2.657984e-03 9.247201e-03 19 35613901 35614034 134 + 1.325 1.057 -0.953
ENSG00000249115 E012 129.4643389 0.0002429651 2.053858e-03 7.393170e-03 19 35614035 35614059 25 + 2.136 2.033 -0.346
ENSG00000249115 E013 8.4917566 0.0019505925 1.324803e-03 5.058922e-03 19 35614060 35614231 172 + 1.150 0.793 -1.333
ENSG00000249115 E014 214.4914631 0.0002024942 6.031625e-04 2.544637e-03 19 35615042 35615147 106 + 2.345 2.255 -0.300
ENSG00000249115 E015 236.3676321 0.0016131392 1.350664e-01 2.411839e-01 19 35615227 35615386 160 + 2.357 2.312 -0.150
ENSG00000249115 E016 155.9845119 0.0021526174 5.421869e-01 6.729895e-01 19 35617124 35617193 70 + 2.162 2.139 -0.075
ENSG00000249115 E017 167.7645570 0.0002650236 1.953715e-02 5.049322e-02 19 35617287 35617369 83 + 2.227 2.157 -0.236
ENSG00000249115 E018 148.9793853 0.0006160104 2.423842e-02 6.030304e-02 19 35617855 35617912 58 + 2.177 2.103 -0.248
ENSG00000249115 E019 223.0839056 0.0005026929 2.338854e-02 5.855310e-02 19 35618071 35618195 125 + 2.342 2.280 -0.205
ENSG00000249115 E020 151.3412747 0.0002156727 7.215177e-01 8.158532e-01 19 35618402 35618465 64 + 2.127 2.136 0.030
ENSG00000249115 E021 210.9484715 0.0002132264 2.717687e-01 4.105527e-01 19 35618569 35618699 131 + 2.256 2.284 0.092
ENSG00000249115 E022 203.8272161 0.0039343022 3.254911e-01 4.689332e-01 19 35618887 35619049 163 + 2.235 2.276 0.135
ENSG00000249115 E023 146.7977402 0.0002758885 1.079395e-03 4.233181e-03 19 35619424 35619504 81 + 2.040 2.146 0.356
ENSG00000249115 E024 198.4499383 0.0002385059 3.203032e-05 1.892303e-04 19 35619613 35619758 146 + 2.162 2.279 0.391
ENSG00000249115 E025 67.9795020 0.0111563140 5.927052e-03 1.841690e-02 19 35619759 35619943 185 + 1.909 1.732 -0.598
ENSG00000249115 E026 36.2514468 0.0068331233 9.512908e-04 3.792172e-03 19 35619944 35620011 68 + 1.680 1.449 -0.789
ENSG00000249115 E027 113.1542809 0.0002963562 8.599916e-06 5.801269e-05 19 35620012 35620057 46 + 1.883 2.051 0.565
ENSG00000249115 E028 131.2423377 0.0003906215 1.164030e-05 7.619447e-05 19 35620058 35620123 66 + 1.956 2.112 0.521
ENSG00000249115 E029 5.3425619 0.0035457491 2.393373e-03 8.440384e-03 19 35620124 35620194 71 + 0.995 0.596 -1.596
ENSG00000249115 E030 208.8475202 0.0013749235 1.028017e-04 5.350255e-04 19 35620195 35620327 133 + 2.178 2.305 0.423
ENSG00000249115 E031 212.3512368 0.0001963636 1.473562e-06 1.180488e-05 19 35622601 35622733 133 + 2.181 2.312 0.438
ENSG00000249115 E032 233.9806449 0.0046789023 1.914058e-05 1.192647e-04 19 35622876 35623057 182 + 2.191 2.370 0.597
ENSG00000249115 E033 740.6850062 0.0029862699 5.129539e-01 6.478332e-01 19 35623058 35625355 2298 + 2.836 2.821 -0.048