ENSG00000248334

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000512149 ENSG00000248334 HEK293_OSMI2_2hA HEK293_TMG_2hB   transcribed_unprocessed_pseudogene processed_transcript 0.628761 0.1996481 1.168529 0.0190831 0.08583067 2.490946 0.46090065 0.18502892 0.6910303 0.00465590 0.04389974 1.845789 0.8049125 0.9385 0.5998 -0.3387 0.03651886 0.03651886 TRUE TRUE
ENST00000515318 ENSG00000248334 HEK293_OSMI2_2hA HEK293_TMG_2hB   transcribed_unprocessed_pseudogene retained_intron 0.628761 0.1996481 1.168529 0.0190831 0.08583067 2.490946 0.09667648 0.00000000 0.2219892 0.00000000 0.08403137 4.535986 0.1163625 0.0000 0.1896 0.1896 0.20076138 0.03651886 FALSE TRUE
ENST00000563942 ENSG00000248334 HEK293_OSMI2_2hA HEK293_TMG_2hB   transcribed_unprocessed_pseudogene transcribed_unprocessed_pseudogene 0.628761 0.1996481 1.168529 0.0190831 0.08583067 2.490946 0.07118383 0.01461914 0.2555092 0.01461914 0.08704792 3.430909 0.0787250 0.0615 0.2106 0.1491 0.41617165 0.03651886 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000248334 E001 1.9103121 0.009043271 0.02542257 0.06275217 15 28737583 28738021 439 + 0.509 0.000 -11.150
ENSG00000248334 E002 1.1760296 0.011797063 0.10465028 0.19758820 15 28738022 28738185 164 + 0.375 0.000 -11.474
ENSG00000248334 E003 0.0000000       15 28741101 28741109 9 +      
ENSG00000248334 E004 2.1047020 0.006962591 0.15439151 0.26761036 15 28741110 28741266 157 + 0.509 0.209 -1.852
ENSG00000248334 E005 0.0000000       15 28741267 28741283 17 +      
ENSG00000248334 E006 1.9617193 0.007608993 0.19081377 0.31476696 15 28744749 28744899 151 + 0.485 0.209 -1.737
ENSG00000248334 E007 3.6467978 0.041345461 0.02476244 0.06138748 15 28745597 28745755 159 + 0.695 0.209 -2.673
ENSG00000248334 E008 8.4520871 0.002444351 0.65042835 0.76146936 15 28745980 28746149 170 + 0.932 0.856 -0.288
ENSG00000248334 E009 4.0944176 0.004096938 0.77999227 0.85855753 15 28747710 28747875 166 + 0.646 0.673 0.112
ENSG00000248334 E010 0.5932625 0.024018201 0.38247356 0.52667448 15 28747876 28748426 551 + 0.227 0.000 -10.599
ENSG00000248334 E011 6.7955120 0.002514195 0.13116713 0.23578122 15 28751488 28751674 187 + 0.789 0.956 0.642
ENSG00000248334 E012 0.0000000       15 28751675 28751678 4 +      
ENSG00000248334 E013 6.6192079 0.002798900 0.92713894 0.95815753 15 28754469 28754555 87 + 0.823 0.817 -0.025
ENSG00000248334 E014 5.0606382 0.003830475 0.12863360 0.23225621 15 28754809 28754869 61 + 0.663 0.856 0.779
ENSG00000248334 E015 25.3664920 0.026337462 0.27096455 0.40964489 15 28754870 28759204 4335 + 1.347 1.408 0.213