ENSG00000245849

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000499988 ENSG00000245849 HEK293_OSMI2_2hA HEK293_TMG_2hB RAD51-AS1 lncRNA retained_intron 6.984924 4.540088 6.456282 0.2203817 0.6236625 0.5070421 0.2809219 0.0000000 1.1267490 0.00000000 0.04377414 6.8287699 0.0476125 0.00000000 0.1785333 0.17853333 2.446830e-13 2.44683e-13 FALSE FALSE
ENST00000526635 ENSG00000245849 HEK293_OSMI2_2hA HEK293_TMG_2hB RAD51-AS1 lncRNA lncRNA 6.984924 4.540088 6.456282 0.2203817 0.6236625 0.5070421 2.1861324 1.5709873 1.8232435 0.19855150 0.16068427 0.2135726 0.3238750 0.34886667 0.2880000 -0.06086667 7.287352e-01 2.44683e-13 FALSE FALSE
ENST00000533146 ENSG00000245849 HEK293_OSMI2_2hA HEK293_TMG_2hB RAD51-AS1 lncRNA lncRNA 6.984924 4.540088 6.456282 0.2203817 0.6236625 0.5070421 0.2084724 0.4038187 0.1681116 0.19809256 0.16811164 -1.2162169 0.0332375 0.08960000 0.0238000 -0.06580000 3.343883e-01 2.44683e-13 FALSE FALSE
ENST00000671605 ENSG00000245849 HEK293_OSMI2_2hA HEK293_TMG_2hB RAD51-AS1 lncRNA lncRNA 6.984924 4.540088 6.456282 0.2203817 0.6236625 0.5070421 0.7912833 0.3387607 0.0000000 0.04468886 0.00000000 -5.1241656 0.1065083 0.07586667 0.0000000 -0.07586667 5.965703e-08 2.44683e-13 FALSE FALSE
MSTRG.10570.4 ENSG00000245849 HEK293_OSMI2_2hA HEK293_TMG_2hB RAD51-AS1 lncRNA   6.984924 4.540088 6.456282 0.2203817 0.6236625 0.5070421 1.1257705 1.2494785 0.9699370 0.40958577 0.53536986 -0.3620656 0.1619208 0.26996667 0.1547333 -0.11523333 7.231375e-01 2.44683e-13   FALSE
MSTRG.10570.5 ENSG00000245849 HEK293_OSMI2_2hA HEK293_TMG_2hB RAD51-AS1 lncRNA   6.984924 4.540088 6.456282 0.2203817 0.6236625 0.5070421 2.2007191 0.7196976 2.2257191 0.17062995 0.73034302 1.6153684 0.2875833 0.16223333 0.3319667 0.16973333 2.912981e-01 2.44683e-13   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000245849 E001 4.349938 0.0104511782 2.718409e-01 0.4106421066 15 40686183 40686242 60 - 0.653 0.821 0.683
ENSG00000245849 E002 32.869034 0.0111957553 4.636025e-05 0.0002633210 15 40686243 40686723 481 - 1.349 1.657 1.055
ENSG00000245849 E003 30.302411 0.0006990718 2.009777e-02 0.0516939848 15 40686724 40687002 279 - 1.418 1.563 0.499
ENSG00000245849 E004 9.895042 0.0041364735 5.405020e-01 0.6716480873 15 40687003 40687033 31 - 0.996 1.066 0.256
ENSG00000245849 E005 17.215302 0.0014826117 6.113957e-01 0.7305028845 15 40687034 40687146 113 - 1.236 1.285 0.171
ENSG00000245849 E006 34.068269 0.0006730949 1.206475e-02 0.0336890116 15 40687147 40687798 652 - 1.614 1.490 -0.424
ENSG00000245849 E007 8.632187 0.0359924739 7.003616e-01 0.7998734626 15 40687799 40687844 46 - 1.010 0.989 -0.075
ENSG00000245849 E008 7.578127 0.0246254953 3.906823e-01 0.5346448732 15 40688707 40688869 163 - 0.996 0.898 -0.367
ENSG00000245849 E009 8.832733 0.0022813626 9.488024e-02 0.1827041477 15 40688870 40688949 80 - 1.084 0.920 -0.608
ENSG00000245849 E010 1.582399 0.1359820338 8.649284e-01 0.9169604737 15 40693734 40693740 7 - 0.413 0.374 -0.218
ENSG00000245849 E011 15.731327 0.0024876473 4.233197e-06 0.0000306094 15 40693741 40694585 845 - 1.385 0.999 -1.372
ENSG00000245849 E012 16.441097 0.0163999173 2.211735e-01 0.3518313373 15 40694586 40694795 210 - 1.296 1.177 -0.420
ENSG00000245849 E013 7.938047 0.0056078905 9.334666e-01 0.9620727541 15 40695012 40695107 96 - 0.937 0.954 0.064