ENSG00000245468

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000499242 ENSG00000245468 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA lncRNA 0.242879 0.2319821 0.1731767 0.1008962 0.03007203 -0.4016642 0.10799869 0.05547262 0.14571010 0.01509620 0.01751084 1.2498988 0.50173333 0.2903667 0.86336667 0.57300000 0.02447287 0.02447287   FALSE
ENST00000501888 ENSG00000245468 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA lncRNA 0.242879 0.2319821 0.1731767 0.1008962 0.03007203 -0.4016642 0.06554895 0.08047104 0.01320784 0.01341559 0.01320784 -1.9628435 0.31840000 0.4242667 0.06140000 -0.36286667 0.23080722 0.02447287 FALSE TRUE
ENST00000504402 ENSG00000245468 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA lncRNA 0.242879 0.2319821 0.1731767 0.1008962 0.03007203 -0.4016642 0.05248047 0.07326110 0.00000000 0.07326110 0.00000000 -3.0576426 0.09478333 0.1690000 0.00000000 -0.16900000 0.66870929 0.02447287   FALSE
ENST00000663544 ENSG00000245468 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA lncRNA 0.242879 0.2319821 0.1731767 0.1008962 0.03007203 -0.4016642 0.01685086 0.02277731 0.01425875 0.01181326 0.01425875 -0.4341922 0.08508333 0.1163667 0.07523333 -0.04113333 1.00000000 0.02447287   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000245468 E001 0.1515154 0.053887138 0.2722729855   4 7093776 7094570 795 - 0.150 0.000 -11.490
ENSG00000245468 E002 0.4782907 0.024931024 0.2203632975 0.350843052 4 7094571 7094743 173 - 0.000 0.248 11.439
ENSG00000245468 E003 0.3697384 0.030432603 0.4175962951 0.560929433 4 7097323 7097379 57 - 0.000 0.180 10.859
ENSG00000245468 E004 3.1397209 0.007418221 0.0002618793 0.001224238 4 7098068 7099454 1387 - 0.830 0.405 -1.901
ENSG00000245468 E005 2.7978996 0.006550973 0.0019028407 0.006923397 4 7101240 7103267 2028 - 0.150 0.686 3.228
ENSG00000245468 E006 2.9514940 0.006395447 0.7635532008 0.846844306 4 7103268 7103329 62 - 0.540 0.611 0.320
ENSG00000245468 E007 1.4757470 0.010160604 0.8444073615 0.903165284 4 7103330 7103385 56 - 0.349 0.405 0.320
ENSG00000245468 E008 0.0000000       4 7103386 7103394 9 -