ENSG00000245146

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000499203 ENSG00000245146 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA lncRNA 1.188638 0.605799 1.575416 0.06739432 0.1240819 1.36433 0.10085987 0.10961112 0.08334857 0.057129797 0.061370400 -0.35765162 0.11480417 0.19383333 0.05943333 -0.13440000 0.6386998735 0.0002913935   FALSE
ENST00000518790 ENSG00000245146 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA lncRNA 1.188638 0.605799 1.575416 0.06739432 0.1240819 1.36433 0.09778828 0.10093035 0.00000000 0.100930347 0.000000000 -3.47158219 0.11351250 0.16693333 0.00000000 -0.16693333 0.5799858414 0.0002913935   FALSE
ENST00000522747 ENSG00000245146 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA lncRNA 1.188638 0.605799 1.575416 0.06739432 0.1240819 1.36433 0.10111164 0.08320750 0.14552773 0.083207501 0.076933792 0.73865389 0.09945000 0.11506667 0.08540000 -0.02966667 0.9286112795 0.0002913935   FALSE
ENST00000654348 ENSG00000245146 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA lncRNA 1.188638 0.605799 1.575416 0.06739432 0.1240819 1.36433 0.03718987 0.00000000 0.07847129 0.000000000 0.078471288 3.14520933 0.06203750 0.00000000 0.05860000 0.05860000 1.0000000000 0.0002913935   FALSE
ENST00000656599 ENSG00000245146 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA lncRNA 1.188638 0.605799 1.575416 0.06739432 0.1240819 1.36433 0.13875020 0.09449311 0.16812553 0.094493105 0.088782350 0.76948655 0.13450833 0.15630000 0.10020000 -0.05610000 0.9333032727 0.0002913935   FALSE
ENST00000659960 ENSG00000245146 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA lncRNA 1.188638 0.605799 1.575416 0.06739432 0.1240819 1.36433 0.17631259 0.00000000 0.62465673 0.000000000 0.105889987 5.98790458 0.13097917 0.00000000 0.41300000 0.41300000 0.0002913935 0.0002913935   FALSE
ENST00000660071 ENSG00000245146 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA lncRNA 1.188638 0.605799 1.575416 0.06739432 0.1240819 1.36433 0.03166120 0.05935476 0.05879009 0.037300728 0.058790085 -0.01179420 0.03058333 0.11023333 0.03613333 -0.07410000 0.6962617541 0.0002913935 TRUE FALSE
ENST00000668151 ENSG00000245146 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA lncRNA 1.188638 0.605799 1.575416 0.06739432 0.1240819 1.36433 0.15781080 0.12700650 0.19541600 0.065305572 0.099169745 0.58430427 0.13485000 0.21170000 0.11603333 -0.09566667 0.8598764242 0.0002913935   FALSE
MSTRG.27032.4 ENSG00000245146 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA   1.188638 0.605799 1.575416 0.06739432 0.1240819 1.36433 0.14887115 0.01820835 0.02010355 0.007574117 0.005948028 0.09381121 0.05078750 0.02793333 0.01240000 -0.01553333 0.6463909563 0.0002913935 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000245146 E001 0.1472490 0.042927230 1.000000000   5 140071312 140072856 1545 - 0.088 0.000 -9.804
ENSG00000245146 E002 0.0000000       5 140072857 140073077 221 -      
ENSG00000245146 E003 0.2214452 0.036444691 0.223169181   5 140101453 140101453 1 - 0.000 0.168 12.520
ENSG00000245146 E004 0.2214452 0.036444691 0.223169181   5 140101454 140101456 3 - 0.000 0.168 12.520
ENSG00000245146 E005 3.2794933 0.024211210 0.302846330 0.444771724 5 140101457 140102224 768 - 0.567 0.719 0.654
ENSG00000245146 E006 6.3078849 0.004562766 0.205342327 0.332855499 5 140102225 140102959 735 - 0.900 0.721 -0.708
ENSG00000245146 E007 31.2616681 0.006451638 0.310761508 0.453192171 5 140102960 140104166 1207 - 1.514 1.440 -0.253
ENSG00000245146 E008 0.1472490 0.042927230 1.000000000   5 140105302 140105310 9 - 0.088 0.000 -11.876
ENSG00000245146 E009 0.1472490 0.042927230 1.000000000   5 140105311 140105354 44 - 0.088 0.000 -11.876
ENSG00000245146 E010 0.5106578 0.290316429 0.279683700 0.419447234 5 140105355 140105424 70 - 0.088 0.290 2.084
ENSG00000245146 E011 1.3231874 0.081321419 0.091376700 0.177276413 5 140105425 140105586 162 - 0.223 0.526 1.810
ENSG00000245146 E012 15.4230211 0.030075596 0.874909918 0.923624637 5 140105587 140105762 176 - 1.211 1.180 -0.110
ENSG00000245146 E013 12.8971622 0.001768195 0.959290726 0.978462595 5 140106296 140106461 166 - 1.132 1.123 -0.032
ENSG00000245146 E014 3.3140384 0.005173674 0.774685737 0.854787522 5 140106462 140106490 29 - 0.640 0.584 -0.247
ENSG00000245146 E015 5.8176435 0.002997054 0.032618794 0.077034239 5 140106491 140107098 608 - 0.702 0.956 0.993
ENSG00000245146 E016 6.7988189 0.003512085 0.710694026 0.807812103 5 140107099 140107408 310 - 0.849 0.882 0.129
ENSG00000245146 E017 3.2442184 0.005679782 0.148570945 0.259726673 5 140107409 140107649 241 - 0.511 0.721 0.925
ENSG00000245146 E018 1.7005162 0.010110437 0.571243112 0.697569882 5 140107650 140108167 518 - 0.370 0.461 0.492
ENSG00000245146 E019 0.0000000       5 140108168 140108190 23 -      
ENSG00000245146 E020 0.1472490 0.042927230 1.000000000   5 140108191 140108472 282 - 0.088 0.000 -11.876
ENSG00000245146 E021 4.1612309 0.004473441 0.001625648 0.006043093 5 140108473 140108603 131 - 0.834 0.289 -2.626
ENSG00000245146 E022 0.5869198 0.023589204 0.171873220 0.290649355 5 140108604 140108664 61 - 0.278 0.000 -13.474
ENSG00000245146 E023 0.7342825 0.017661718 0.104917531 0.197971230 5 140108760 140109136 377 - 0.327 0.000 -13.703
ENSG00000245146 E024 0.2924217 0.029078516 0.507731739   5 140109137 140109264 128 - 0.161 0.000 -12.718
ENSG00000245146 E025 0.8157519 0.016002794 0.689920752 0.791738853 5 140109265 140110374 1110 - 0.223 0.289 0.490
ENSG00000245146 E026 2.8430303 0.005759854 0.067492217 0.139177076 5 140114011 140119401 5391 - 0.446 0.720 1.245