ENSG00000239521

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000328453 ENSG00000239521 HEK293_OSMI2_2hA HEK293_TMG_2hB CASTOR3 transcribed_unprocessed_pseudogene processed_transcript 5.300197 7.31593 3.83755 0.3520501 0.515441 -0.929072 1.1291706 1.03639672 1.0527137 0.10649592 0.12898053 0.02232307 0.22425000 0.141533333 0.28020000 0.138666667 0.019978447 0.002540765 TRUE TRUE
ENST00000414739 ENSG00000239521 HEK293_OSMI2_2hA HEK293_TMG_2hB CASTOR3 transcribed_unprocessed_pseudogene processed_transcript 5.300197 7.31593 3.83755 0.3520501 0.515441 -0.929072 2.2938660 3.57599407 1.0842224 0.03094362 0.02465753 -1.71246705 0.41946667 0.490933333 0.29196667 -0.198966667 0.006686972 0.002540765 TRUE TRUE
ENST00000445230 ENSG00000239521 HEK293_OSMI2_2hA HEK293_TMG_2hB CASTOR3 transcribed_unprocessed_pseudogene processed_transcript 5.300197 7.31593 3.83755 0.3520501 0.515441 -0.929072 0.3221380 0.11223224 0.3193731 0.06460284 0.16831293 1.43009766 0.06272917 0.014766667 0.07406667 0.059300000 0.568105686 0.002540765   TRUE
ENST00000538588 ENSG00000239521 HEK293_OSMI2_2hA HEK293_TMG_2hB CASTOR3 transcribed_unprocessed_pseudogene processed_transcript 5.300197 7.31593 3.83755 0.3520501 0.515441 -0.929072 0.2611951 0.00000000 0.0000000 0.00000000 0.00000000 0.00000000 0.05332500 0.000000000 0.00000000 0.000000000   0.002540765   FALSE
ENST00000543273 ENSG00000239521 HEK293_OSMI2_2hA HEK293_TMG_2hB CASTOR3 transcribed_unprocessed_pseudogene processed_transcript 5.300197 7.31593 3.83755 0.3520501 0.515441 -0.929072 0.2525906 0.73240476 0.3463024 0.11067295 0.18017759 -1.05910367 0.05267917 0.100633333 0.10190000 0.001266667 0.895545235 0.002540765 TRUE TRUE
ENST00000689058 ENSG00000239521 HEK293_OSMI2_2hA HEK293_TMG_2hB CASTOR3 transcribed_unprocessed_pseudogene processed_transcript 5.300197 7.31593 3.83755 0.3520501 0.515441 -0.929072 0.7625741 1.62899730 0.3196864 0.27490151 0.10104259 -2.31364712 0.13061250 0.220200000 0.08430000 -0.135900000 0.030354936 0.002540765 FALSE TRUE
MSTRG.30322.8 ENSG00000239521 HEK293_OSMI2_2hA HEK293_TMG_2hB CASTOR3 transcribed_unprocessed_pseudogene   5.300197 7.31593 3.83755 0.3520501 0.515441 -0.929072 0.1402977 0.05143244 0.5837743 0.05143244 0.27007470 3.27284222 0.03107500 0.006433333 0.13996667 0.133533333 0.002540765 0.002540765 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000239521 E001 77.765634 0.0054992757 1.781865e-01 2.988779e-01 7 100200653 100202212 1560 - 1.901 1.836 -0.218
ENSG00000239521 E002 6.556002 0.0026083999 5.837773e-02 1.238478e-01 7 100202213 100202240 28 - 0.993 0.762 -0.884
ENSG00000239521 E003 7.583761 0.0025667400 7.561376e-02 1.524872e-01 7 100202241 100202289 49 - 1.032 0.826 -0.776
ENSG00000239521 E004 6.455983 0.0028056112 4.334178e-02 9.718736e-02 7 100202290 100202312 23 - 0.993 0.745 -0.954
ENSG00000239521 E005 10.868651 0.0016847006 2.789214e-02 6.780005e-02 7 100202313 100202512 200 - 1.174 0.953 -0.805
ENSG00000239521 E006 6.449511 0.0026769128 1.343846e-01 2.402517e-01 7 100202513 100202603 91 - 0.949 0.763 -0.722
ENSG00000239521 E007 20.096378 0.0010100574 3.868412e-05 2.237259e-04 7 100211394 100211583 190 - 1.481 1.173 -1.075
ENSG00000239521 E008 25.505716 0.0010547431 2.282730e-04 1.084421e-03 7 100211584 100211934 351 - 1.537 1.284 -0.875
ENSG00000239521 E009 9.114751 0.0080032614 5.537453e-02 1.186350e-01 7 100211935 100211994 60 - 1.101 0.883 -0.809
ENSG00000239521 E010 64.635653 0.0014818717 4.507574e-07 4.022546e-06 7 100212022 100212952 931 - 1.576 1.838 0.886
ENSG00000239521 E011 26.321506 0.0007712552 4.122664e-04 1.821466e-03 7 100212953 100213046 94 - 1.174 1.453 0.973
ENSG00000239521 E012 37.151324 0.0005960827 3.450785e-01 4.891623e-01 7 100213047 100213133 87 - 1.494 1.552 0.198
ENSG00000239521 E013 8.665974 0.0019842947 1.215998e-03 4.694471e-03 7 100214970 100215179 210 - 1.161 0.811 -1.301
ENSG00000239521 E014 33.681705 0.0016439472 6.547507e-01 7.648515e-01 7 100222597 100222644 48 - 1.481 1.511 0.102
ENSG00000239521 E015 37.985002 0.0005965898 8.862165e-01 9.312278e-01 7 100222645 100222720 76 - 1.543 1.552 0.030
ENSG00000239521 E016 11.680977 0.0016938467 1.313647e-04 6.648849e-04 7 100223295 100223569 275 - 0.674 1.146 1.806
ENSG00000239521 E017 60.324437 0.0014992516 5.682367e-02 1.211554e-01 7 100223570 100223698 129 - 1.668 1.764 0.327
ENSG00000239521 E018 72.156669 0.0049388220 4.350760e-01 5.773150e-01 7 100223882 100224075 194 - 1.792 1.831 0.132
ENSG00000239521 E019 40.641238 0.0007924163 4.228635e-01 5.660059e-01 7 100233604 100233674 71 - 1.537 1.584 0.161
ENSG00000239521 E020 0.000000       7 100246462 100246548 87 -      
ENSG00000239521 E021 34.744550 0.0006762853 1.864928e-01 3.093467e-01 7 100271838 100272218 381 - 1.560 1.480 -0.275