ENSG00000235750

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000423313 ENSG00000235750 HEK293_OSMI2_2hA HEK293_TMG_2hB KIAA0040 protein_coding protein_coding 2.149133 1.718407 2.737566 0.2403048 0.2449447 0.6687116 1.40555393 0.9683218 1.6864616 0.38935331 0.1713605 0.7941479 0.65938333 0.55986667 0.61500000 0.05513333 1.000000e+00 1.099225e-06 FALSE TRUE
ENST00000444639 ENSG00000235750 HEK293_OSMI2_2hA HEK293_TMG_2hB KIAA0040 protein_coding protein_coding 2.149133 1.718407 2.737566 0.2403048 0.2449447 0.6687116 0.17002321 0.1405151 0.1757527 0.07034051 0.1757527 0.3034756 0.08523333 0.08166667 0.07466667 -0.00700000 6.702234e-01 1.099225e-06 FALSE TRUE
ENST00000545251 ENSG00000235750 HEK293_OSMI2_2hA HEK293_TMG_2hB KIAA0040 protein_coding protein_coding 2.149133 1.718407 2.737566 0.2403048 0.2449447 0.6687116 0.33145460 0.0000000 0.6004248 0.00000000 0.1243368 5.9317418 0.14482500 0.00000000 0.21853333 0.21853333 1.099225e-06 1.099225e-06 FALSE TRUE
ENST00000563563 ENSG00000235750 HEK293_OSMI2_2hA HEK293_TMG_2hB KIAA0040 protein_coding processed_transcript 2.149133 1.718407 2.737566 0.2403048 0.2449447 0.6687116 0.13376123 0.0000000 0.1830516 0.00000000 0.1830516 4.2709146 0.05275417 0.00000000 0.05733333 0.05733333 8.703066e-01 1.099225e-06   FALSE
ENST00000619513 ENSG00000235750 HEK293_OSMI2_2hA HEK293_TMG_2hB KIAA0040 protein_coding protein_coding 2.149133 1.718407 2.737566 0.2403048 0.2449447 0.6687116 0.07619623 0.6095698 0.0000000 0.30791549 0.0000000 -5.9531950 0.04480833 0.35846667 0.00000000 -0.35846667 1.244807e-01 1.099225e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000235750 E001 0.000000       1 175156986 175156986 1 -      
ENSG00000235750 E002 101.143459 0.0061628271 1.173606e-05 7.673909e-05 1 175156987 175158952 1966 - 1.911 2.103 0.646
ENSG00000235750 E003 120.166855 0.0004371841 1.939087e-01 3.186958e-01 1 175158953 175161026 2074 - 2.091 2.086 -0.016
ENSG00000235750 E004 8.463198 0.0023009398 1.093288e-02 3.100044e-02 1 175161027 175161043 17 - 1.091 0.834 -0.958
ENSG00000235750 E005 15.297531 0.0013508828 4.904142e-03 1.566314e-02 1 175161044 175161146 103 - 1.305 1.090 -0.762
ENSG00000235750 E006 15.620040 0.0013508828 1.879048e-02 4.887485e-02 1 175166562 175166737 176 - 1.298 1.127 -0.607
ENSG00000235750 E007 0.000000       1 175166738 175166741 4 -      
ENSG00000235750 E008 0.294498 0.4512807146 3.794859e-01   1 175176400 175176474 75 - 0.199 0.000 -9.543
ENSG00000235750 E009 8.047752 0.0021602084 5.203253e-02 1.127747e-01 1 175177611 175177684 74 - 1.048 0.857 -0.715
ENSG00000235750 E010 7.722484 0.0047469385 1.635037e-01 2.797149e-01 1 175192640 175192716 77 - 1.013 0.878 -0.504
ENSG00000235750 E011 4.060930 0.0041317579 4.434032e-01 5.849843e-01 1 175192717 175192776 60 - 0.752 0.667 -0.351
ENSG00000235750 E012 1.586578 0.0095466095 8.010991e-01 8.732449e-01 1 175192860 175192999 140 - 0.390 0.450 0.327