ENSG00000235618

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000456967 ENSG00000235618 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM21EP transcribed_unprocessed_pseudogene processed_transcript 0.2920756 0.338613 0.3702148 0.0145709 0.07905355 0.125188 0.21161928 0.29938842 0.18000340 0.03269255 0.04370549 -0.7033940 0.73097500 0.8796667 0.4838333 -0.3958333 0.03884956 0.03884956 TRUE FALSE
ENST00000649244 ENSG00000235618 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM21EP transcribed_unprocessed_pseudogene processed_transcript 0.2920756 0.338613 0.3702148 0.0145709 0.07905355 0.125188 0.02527125 0.00000000 0.13938610 0.00000000 0.07213774 3.9009740 0.08052917 0.0000000 0.3165667 0.3165667 0.11550463 0.03884956   FALSE
MSTRG.4004.1 ENSG00000235618 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM21EP transcribed_unprocessed_pseudogene   0.2920756 0.338613 0.3702148 0.0145709 0.07905355 0.125188 0.04702100 0.03922463 0.05082528 0.01961981 0.03406728 0.3052907 0.16555417 0.1203333 0.1996333 0.0793000 0.98570308 0.03884956 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000235618 E001 0.1482932 0.046725025 0.3591148939   10 50009475 50010212 738 - 0.000 0.161 10.671
ENSG00000235618 E002 0.8168978 0.015830337 0.2997354092 0.441314790 10 50021182 50021400 219 - 0.288 0.161 -1.068
ENSG00000235618 E003 0.3729606 0.029391004 0.8077341686 0.877837143 10 50021401 50021467 67 - 0.092 0.161 0.934
ENSG00000235618 E004 1.5081155 0.013506670 0.8978182950 0.938770212 10 50048202 50048333 132 - 0.383 0.446 0.343
ENSG00000235618 E005 1.8143686 0.008505119 0.2112786051 0.340003171 10 50050532 50050633 102 - 0.494 0.370 -0.656
ENSG00000235618 E006 1.8174891 0.009429498 0.5332713587 0.665489692 10 50054638 50054724 87 - 0.460 0.446 -0.072
ENSG00000235618 E007 1.1856066 0.011735804 0.0667826637 0.138039001 10 50056784 50056911 128 - 0.423 0.161 -1.878
ENSG00000235618 E008 1.9907493 0.009712564 0.1437508971 0.253117605 10 50059101 50059273 173 - 0.526 0.371 -0.807
ENSG00000235618 E009 1.1156622 0.013635001 0.1112013710 0.207315372 10 50059665 50059724 60 - 0.383 0.161 -1.653
ENSG00000235618 E010 0.5954526 0.019079637 0.0720512765 0.146743996 10 50060947 50061004 58 - 0.288 0.000 -13.668
ENSG00000235618 E011 1.4864473 0.010864776 0.0233192525 0.058413604 10 50061478 50061596 119 - 0.494 0.161 -2.240
ENSG00000235618 E012 0.7469680 0.018017618 0.0375955060 0.086571888 10 50062636 50062704 69 - 0.338 0.000 -13.899
ENSG00000235618 E013 0.0000000       10 50063320 50063403 84 -      
ENSG00000235618 E014 0.2987644 0.031668702 0.2993113041   10 50063947 50064057 111 - 0.168 0.000 -12.928
ENSG00000235618 E015 12.0088429 0.173707032 0.0004015539 0.001780447 10 50066526 50067803 1278 - 0.790 1.358 2.077