ENSG00000235173

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000347708 ENSG00000235173 HEK293_OSMI2_2hA HEK293_TMG_2hB HGH1 protein_coding protein_coding 40.12464 69.84976 21.22135 3.091363 0.9130865 -1.718266 11.65012 12.362545 11.294215 2.4716054 0.5282479 -0.1302815 0.31340000 0.1751333 0.5357333 0.3606000 1.020374e-05 1.837082e-10 FALSE TRUE
ENST00000525101 ENSG00000235173 HEK293_OSMI2_2hA HEK293_TMG_2hB HGH1 protein_coding retained_intron 40.12464 69.84976 21.22135 3.091363 0.9130865 -1.718266 6.24355 5.656642 6.409453 0.9341198 0.5690289 0.1799563 0.19389167 0.0801000 0.3034333 0.2233333 1.157181e-08 1.837082e-10 FALSE TRUE
ENST00000533266 ENSG00000235173 HEK293_OSMI2_2hA HEK293_TMG_2hB HGH1 protein_coding nonsense_mediated_decay 40.12464 69.84976 21.22135 3.091363 0.9130865 -1.718266 18.20207 41.989192 2.748934 3.1101340 1.1206599 -3.9281789 0.40640417 0.5999667 0.1256333 -0.4743333 3.988798e-05 1.837082e-10 FALSE TRUE
ENST00000534255 ENSG00000235173 HEK293_OSMI2_2hA HEK293_TMG_2hB HGH1 protein_coding retained_intron 40.12464 69.84976 21.22135 3.091363 0.9130865 -1.718266 3.42772 9.332359 0.000000 2.7939864 0.0000000 -9.8676431 0.06860417 0.1377333 0.0000000 -0.1377333 1.837082e-10 1.837082e-10 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000235173 E001 48.085213 0.0185891627 3.454797e-03 1.159476e-02 8 144137774 144137803 30 + 1.753 1.546 -0.702
ENSG00000235173 E002 51.678933 0.0216778807 4.690357e-03 1.507003e-02 8 144137804 144137809 6 + 1.783 1.579 -0.692
ENSG00000235173 E003 70.738390 0.0178870224 6.687916e-04 2.784200e-03 8 144137810 144137835 26 + 1.928 1.707 -0.747
ENSG00000235173 E004 95.448844 0.0195321379 9.797750e-06 6.528399e-05 8 144137836 144138018 183 + 2.106 1.813 -0.985
ENSG00000235173 E005 62.467042 0.0190353980 5.275774e-13 1.353025e-11 8 144138019 144138289 271 + 2.067 1.531 -1.811
ENSG00000235173 E006 135.925259 0.0011244679 8.749775e-05 4.632808e-04 8 144138290 144138428 139 + 2.129 2.017 -0.377
ENSG00000235173 E007 2.039047 0.1936009789 1.633584e-01 2.795541e-01 8 144138466 144138506 41 + 0.649 0.328 -1.616
ENSG00000235173 E008 210.524055 0.0007820302 4.908844e-01 6.282066e-01 8 144138507 144138607 101 + 2.185 2.241 0.190
ENSG00000235173 E009 2.469039 0.0079179882 1.251179e-02 3.476308e-02 8 144138608 144138614 7 + 0.747 0.361 -1.826
ENSG00000235173 E010 8.459755 0.0171307924 2.146673e-05 1.321217e-04 8 144138683 144138714 32 + 1.232 0.736 -1.854
ENSG00000235173 E011 259.050157 0.0011195357 9.871818e-01 9.960409e-01 8 144138715 144138758 44 + 2.292 2.328 0.118
ENSG00000235173 E012 303.806979 0.0001967878 2.108004e-01 3.394982e-01 8 144138759 144138813 55 + 2.387 2.390 0.012
ENSG00000235173 E013 49.997125 0.0027142395 3.279158e-18 1.693639e-16 8 144138814 144139008 195 + 1.928 1.478 -1.528
ENSG00000235173 E014 383.684850 0.0001815765 8.146730e-01 8.825834e-01 8 144139009 144139100 92 + 2.467 2.496 0.097
ENSG00000235173 E015 13.116071 0.0013242843 4.990657e-01 6.354137e-01 8 144139101 144139108 8 + 1.096 1.055 -0.148
ENSG00000235173 E016 499.627078 0.0007399699 2.217412e-01 3.524770e-01 8 144139189 144139311 123 + 2.558 2.620 0.208
ENSG00000235173 E017 2245.638154 0.0028557795 2.126950e-09 2.964134e-08 8 144139388 144140851 1464 + 3.156 3.287 0.437