ENSG00000234545

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000415397 ENSG00000234545 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM133B protein_coding nonsense_mediated_decay 30.44428 13.43935 54.91692 1.010394 3.81247 2.029977 6.8056811 3.10726416 10.197367 0.44091528 2.4377224 1.711259 0.23365417 0.22886667 0.19330000 -0.03556667 7.633758e-01 2.707569e-08 TRUE FALSE
ENST00000445716 ENSG00000234545 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM133B protein_coding protein_coding 30.44428 13.43935 54.91692 1.010394 3.81247 2.029977 15.7169477 9.74445584 26.432559 0.71807416 3.0328797 1.438729 0.56727083 0.72540000 0.47846667 -0.24693333 2.014406e-07 2.707569e-08 FALSE TRUE
ENST00000481407 ENSG00000234545 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM133B protein_coding retained_intron 30.44428 13.43935 54.91692 1.010394 3.81247 2.029977 2.1028537 0.33621638 4.261689 0.08023779 0.5884390 3.625061 0.05889167 0.02623333 0.07900000 0.05276667 2.057111e-02 2.707569e-08 TRUE FALSE
MSTRG.30217.5 ENSG00000234545 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM133B protein_coding   30.44428 13.43935 54.91692 1.010394 3.81247 2.029977 3.3002386 0.02545151 4.506801 0.02545151 0.9218551 6.993310 0.07485417 0.00170000 0.08236667 0.08066667 2.707569e-08 2.707569e-08 FALSE TRUE
MSTRG.30217.6 ENSG00000234545 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM133B protein_coding   30.44428 13.43935 54.91692 1.010394 3.81247 2.029977 0.7548691 0.00000000 6.038953 0.00000000 3.1761803 9.240542 0.01288333 0.00000000 0.10306667 0.10306667 2.908663e-01 2.707569e-08 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000234545 E001 2.0787768 0.0077583239 6.249680e-01 7.411753e-01 7 92560758 92560792 35 - 0.399 0.493 0.490
ENSG00000234545 E002 36.3556427 0.0265377409 5.839994e-06 4.094783e-05 7 92560793 92561136 344 - 1.341 1.804 1.584
ENSG00000234545 E003 14.2551826 0.0012757083 8.175662e-06 5.541123e-05 7 92561137 92561215 79 - 0.974 1.397 1.506
ENSG00000234545 E004 65.6288210 0.0005912319 7.802432e-12 1.653779e-10 7 92561216 92561749 534 - 1.642 1.973 1.116
ENSG00000234545 E005 51.0172111 0.0027649860 4.206039e-01 5.638061e-01 7 92561750 92562050 301 - 1.614 1.670 0.187
ENSG00000234545 E006 167.4083644 0.0011503224 9.197393e-03 2.677842e-02 7 92562051 92562281 231 - 2.112 2.207 0.316
ENSG00000234545 E007 119.3474549 0.0005763224 2.251525e-01 3.566086e-01 7 92562282 92562368 87 - 1.975 2.025 0.168
ENSG00000234545 E008 14.8516972 0.0015800235 7.366608e-03 2.217039e-02 7 92563852 92563952 101 - 1.163 0.822 -1.265
ENSG00000234545 E009 14.0063081 0.0012139476 1.561736e-03 5.835396e-03 7 92565508 92566009 502 - 1.149 0.718 -1.631
ENSG00000234545 E010 7.1520951 0.0204900995 9.259143e-02 1.791707e-01 7 92566010 92566013 4 - 0.873 0.582 -1.198
ENSG00000234545 E011 97.3857724 0.0021266163 4.981808e-02 1.088373e-01 7 92566014 92566049 36 - 1.919 1.819 -0.337
ENSG00000234545 E012 91.7676217 0.0009041175 2.460747e-02 6.106929e-02 7 92566050 92566061 12 - 1.895 1.784 -0.374
ENSG00000234545 E013 4.2837033 0.0036529801 1.530751e-03 5.736250e-03 7 92569542 92569822 281 - 0.729 0.000 -13.577
ENSG00000234545 E014 138.2024813 0.0012327552 4.955190e-02 1.083677e-01 7 92569823 92569915 93 - 2.067 1.986 -0.272
ENSG00000234545 E015 134.1964620 0.0006909850 5.187322e-03 1.643816e-02 7 92575771 92575821 51 - 2.060 1.946 -0.383
ENSG00000234545 E016 0.2966881 0.0274424043 1.000000e+00   7 92575822 92575854 33 - 0.114 0.000 -9.764
ENSG00000234545 E017 194.6516606 0.0002666892 9.130412e-01 9.489043e-01 7 92577103 92577195 93 - 2.199 2.197 -0.007
ENSG00000234545 E018 1.0654260 0.0867014284 8.588154e-01 9.129115e-01 7 92577196 92577394 199 - 0.279 0.232 -0.356
ENSG00000234545 E019 130.2812187 0.0002611762 2.982954e-01 4.397414e-01 7 92577655 92577676 22 - 2.015 2.056 0.137
ENSG00000234545 E020 121.2697052 0.0016699463 5.513827e-02 1.182346e-01 7 92577677 92577717 41 - 1.975 2.059 0.280
ENSG00000234545 E021 27.2361502 0.0203874535 1.388123e-02 3.795730e-02 7 92577718 92578149 432 - 1.405 1.115 -1.021
ENSG00000234545 E022 102.9284061 0.0003229986 3.542858e-01 4.985623e-01 7 92578150 92578182 33 - 1.915 1.956 0.138
ENSG00000234545 E023 177.0369824 0.0001906567 8.919912e-01 9.350294e-01 7 92578319 92578393 75 - 2.156 2.162 0.020
ENSG00000234545 E024 0.0000000       7 92579155 92579316 162 -      
ENSG00000234545 E025 184.0818942 0.0004516443 2.779333e-01 4.175745e-01 7 92579317 92579395 79 - 2.180 2.146 -0.116
ENSG00000234545 E026 200.1896084 0.0006207121 3.951671e-02 9.013692e-02 7 92581506 92581603 98 - 2.222 2.156 -0.221
ENSG00000234545 E027 0.5911862 0.0193874923 4.986127e-01 6.350768e-01 7 92581604 92581806 203 - 0.205 0.000 -10.754
ENSG00000234545 E028 5.8462088 0.0636614572 4.356767e-02 9.758823e-02 7 92585298 92585396 99 - 0.818 0.382 -1.982
ENSG00000234545 E029 0.4375944 0.0247765437 7.329826e-01 8.243183e-01 7 92589860 92589875 16 - 0.162 0.000 -10.345
ENSG00000234545 E030 1.1739533 0.0119980132 1.607870e-01 2.761514e-01 7 92589876 92590267 392 - 0.343 0.000 -11.742
ENSG00000234545 E031 150.8250094 0.0020356663 8.618207e-03 2.533271e-02 7 92590268 92590393 126 - 2.108 1.999 -0.365