ENSG00000232040

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000452236 ENSG00000232040 HEK293_OSMI2_2hA HEK293_TMG_2hB ZBED9 protein_coding protein_coding 3.227672 0.4186236 5.806188 0.006257029 0.3444361 3.762291 2.1111063 0.4032935 3.396155 0.01904238 0.08104438 3.042905 0.7778792 0.9628667 0.5875667 -0.3753000 7.497871e-05 1.587871e-05 FALSE TRUE
MSTRG.27908.3 ENSG00000232040 HEK293_OSMI2_2hA HEK293_TMG_2hB ZBED9 protein_coding   3.227672 0.4186236 5.806188 0.006257029 0.3444361 3.762291 0.9060923 0.0000000 1.908729 0.00000000 0.12933330 7.584007 0.1606958 0.0000000 0.3296667 0.3296667 1.587871e-05 1.587871e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000232040 E001 139.990175 0.0003097396 4.422017e-13 1.149476e-11 6 28570535 28573683 3149 - 1.921 2.139 0.732
ENSG00000232040 E002 20.741777 0.0009128260 2.599582e-01 3.974426e-01 6 28573684 28573827 144 - 1.164 1.002 -0.586
ENSG00000232040 E003 99.235442 0.0087216003 7.838513e-05 4.203416e-04 6 28574644 28576016 1373 - 1.829 1.523 -1.039
ENSG00000232040 E004 17.423485 0.0011514304 3.687178e-03 1.225912e-02 6 28576017 28576136 120 - 1.114 0.604 -1.993
ENSG00000232040 E005 1.927378 0.0552546271 2.656469e-01 4.037127e-01 6 28579243 28579271 29 - 0.375 0.001 -9.573
ENSG00000232040 E006 27.371817 0.0007730345 4.421833e-03 1.432358e-02 6 28579272 28579419 148 - 1.291 0.905 -1.397
ENSG00000232040 E007 1.442539 0.0096659056 2.395618e-01 3.736007e-01 6 28585206 28585321 116 - 0.240 0.479 1.449
ENSG00000232040 E008 75.329377 0.0005044885 5.421257e-01 6.729443e-01 6 28586298 28587298 1001 - 1.692 1.636 -0.192
ENSG00000232040 E009 4.196179 0.0078214326 7.748294e-01 8.548963e-01 6 28587462 28587596 135 - 0.560 0.604 0.195
ENSG00000232040 E010 0.147249 0.0441990074 5.795439e-01   6 28587956 28588006 51 - 0.043 0.001 -5.942
ENSG00000232040 E011 0.000000       6 28588007 28588119 113 -      
ENSG00000232040 E012 0.000000       6 28589360 28590541 1182 -      
ENSG00000232040 E013 0.000000       6 28593550 28593647 98 -      
ENSG00000232040 E014 0.000000       6 28594212 28594247 36 -      
ENSG00000232040 E015 0.000000       6 28597724 28598030 307 -      
ENSG00000232040 E016 0.000000       6 28616025 28616212 188 -