ENSG00000231389

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000437811 ENSG00000231389 HEK293_OSMI2_2hA HEK293_TMG_2hB HLA-DPA1 protein_coding protein_coding 8.16562 11.96976 4.318596 0.01663013 0.3570173 -1.468628 0.5660132 0.5681283 0.5997414 0.1224524 0.002625197 0.07680785 0.0910375 0.0475000 0.1409667 0.09346667 0.002271301 0.002271301 FALSE FALSE
ENST00000453337 ENSG00000231389 HEK293_OSMI2_2hA HEK293_TMG_2hB HLA-DPA1 protein_coding protein_coding 8.16562 11.96976 4.318596 0.01663013 0.3570173 -1.468628 2.7195686 3.6580042 1.7928623 0.5033748 0.338628918 -1.02470609 0.3538833 0.3054667 0.4073000 0.10183333 0.432897483 0.002271301 FALSE FALSE
ENST00000692443 ENSG00000231389 HEK293_OSMI2_2hA HEK293_TMG_2hB HLA-DPA1 protein_coding protein_coding 8.16562 11.96976 4.318596 0.01663013 0.3570173 -1.468628 4.8380100 7.6474491 1.8914620 0.4007060 0.041724123 -2.00975480 0.5483583 0.6389667 0.4432667 -0.19570000 0.014917143 0.002271301 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000231389 E001 0.0000000       6 33064569 33065006 438 -      
ENSG00000231389 E002 82.7024949 0.0003713017 8.029751e-03 2.385617e-02 6 33065007 33065347 341 - 1.720 1.842 0.413
ENSG00000231389 E003 3.2494286 0.0697662997 5.558279e-02 1.189763e-01 6 33065348 33067659 2312 - 0.814 0.461 -1.543
ENSG00000231389 E004 16.7645345 0.0174887378 2.664271e-06 2.017339e-05 6 33067660 33068637 978 - 1.491 1.024 -1.650
ENSG00000231389 E005 69.4270639 0.0006263916 2.122251e-01 3.411488e-01 6 33068638 33068804 167 - 1.693 1.754 0.204
ENSG00000231389 E006 0.5901394 0.3844851792 6.731279e-01 7.789082e-01 6 33068805 33069018 214 - 0.207 0.134 -0.757
ENSG00000231389 E007 58.5034184 0.0038138192 5.696536e-02 1.214007e-01 6 33069019 33069103 85 - 1.577 1.693 0.395
ENSG00000231389 E008 64.8857760 0.0004436815 9.577093e-02 1.840505e-01 6 33069104 33069161 58 - 1.648 1.733 0.288
ENSG00000231389 E009 94.9355209 0.0003197215 6.509673e-01 7.619354e-01 6 33069162 33069300 139 - 1.865 1.880 0.052
ENSG00000231389 E010 0.2965864 0.1550561816 1.000000e+00   6 33069301 33069640 340 - 0.000 0.127 8.998
ENSG00000231389 E011 123.1951453 0.0004222532 5.699400e-01 6.964589e-01 6 33069641 33069886 246 - 2.009 1.985 -0.079
ENSG00000231389 E012 0.1817044 0.0399509660 1.000000e+00   6 33073439 33073470 32 - 0.000 0.069 8.648
ENSG00000231389 E013 48.2537207 0.0005636400 7.785882e-02 1.560413e-01 6 33073471 33073501 31 - 1.671 1.568 -0.352
ENSG00000231389 E014 57.6243590 0.0004325030 3.302442e-02 7.785243e-02 6 33073502 33073669 168 - 1.754 1.641 -0.383
ENSG00000231389 E015 0.5503986 0.0202783405 6.431649e-01 7.557708e-01 6 33073670 33073710 41 - 0.207 0.129 -0.821
ENSG00000231389 E016 0.0000000       6 33073711 33073772 62 -      
ENSG00000231389 E017 0.2214452 0.0407659824 1.000000e+00   6 33080680 33080775 96 - 0.000 0.069 8.645