ENSG00000228506

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000418945 ENSG00000228506 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA lncRNA 3.548568 2.662002 3.586006 0.353669 0.2553908 0.4284743 2.0478057 1.6232698 2.1546556 0.09306812 0.31102888 0.4063744 0.58855000 0.63623333 0.61726667 -0.01896667 0.998100370 0.002867694 FALSE FALSE
ENST00000693615 ENSG00000228506 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA lncRNA 3.548568 2.662002 3.586006 0.353669 0.2553908 0.4284743 0.5937871 0.1843024 0.7441424 0.03996059 0.10194158 1.9565336 0.15190417 0.07226667 0.20553333 0.13326667 0.002867694 0.002867694   FALSE
MSTRG.28689.1 ENSG00000228506 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA   3.548568 2.662002 3.586006 0.353669 0.2553908 0.4284743 0.1993931 0.2423587 0.1243206 0.14283512 0.06667968 -0.9097952 0.06627083 0.10050000 0.03233333 -0.06816667 0.800126789 0.002867694 FALSE FALSE
MSTRG.28689.5 ENSG00000228506 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA   3.548568 2.662002 3.586006 0.353669 0.2553908 0.4284743 0.5782494 0.5610863 0.3924459 0.56108627 0.39244589 -0.5049139 0.15141667 0.17006667 0.09733333 -0.07273333 0.966477499 0.002867694 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000228506 E001 0.147249 0.0436498779 6.051379e-01   6 99424911 99424912 2 + 0.107 0.000 -8.846
ENSG00000228506 E002 22.232958 0.0371030320 2.755314e-01 0.4148540959 6 99424913 99425387 475 + 1.398 1.310 -0.306
ENSG00000228506 E003 12.066883 0.0016110765 1.945186e-01 0.3194049376 6 99425388 99425478 91 + 1.150 1.065 -0.306
ENSG00000228506 E004 19.531127 0.0028916863 1.847117e-02 0.0481659980 6 99425479 99425560 82 + 1.373 1.229 -0.506
ENSG00000228506 E005 22.635434 0.0097340748 2.145352e-01 0.3439000252 6 99425561 99425645 85 + 1.403 1.339 -0.223
ENSG00000228506 E006 23.024624 0.0010112702 3.967668e-01 0.5405307386 6 99425646 99425731 86 + 1.388 1.369 -0.069
ENSG00000228506 E007 30.198658 0.0008021709 5.993259e-01 0.7207501197 6 99425732 99425898 167 + 1.498 1.506 0.029
ENSG00000228506 E008 4.960522 0.0036033691 5.132811e-04 0.0022093876 6 99425899 99426061 163 + 0.932 0.462 -1.991
ENSG00000228506 E009 5.064905 0.0046475146 1.668683e-01 0.2841131925 6 99426062 99426160 99 + 0.837 0.681 -0.628
ENSG00000228506 E010 6.470607 0.0026894394 7.793441e-01 0.8580834347 6 99426161 99426554 394 + 0.871 0.872 0.005
ENSG00000228506 E011 118.323199 0.0093467003 3.920585e-05 0.0002264668 6 99430713 99432326 1614 + 1.981 2.168 0.626
ENSG00000228506 E012 3.325793 0.0049079851 4.948416e-02 0.1082457815 6 99433019 99434105 1087 + 0.738 0.462 -1.236