ENSG00000227354

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000456602 ENSG00000227354 HEK293_OSMI2_2hA HEK293_TMG_2hB RBM26-AS1 lncRNA lncRNA 4.156989 2.411447 6.612283 0.436097 0.1793087 1.451459 0.2891789 0.20031867 0.3707860 0.07783863 0.17366055 0.8564033 0.07590417 0.09603333 0.0565000 -0.03953333 0.81930411 0.02931751 FALSE TRUE
ENST00000606584 ENSG00000227354 HEK293_OSMI2_2hA HEK293_TMG_2hB RBM26-AS1 lncRNA lncRNA 4.156989 2.411447 6.612283 0.436097 0.1793087 1.451459 0.9791107 0.73517058 0.8146442 0.25010014 0.14666686 0.1462011 0.23024583 0.28203333 0.1245000 -0.15753333 0.12249042 0.02931751   FALSE
ENST00000607864 ENSG00000227354 HEK293_OSMI2_2hA HEK293_TMG_2hB RBM26-AS1 lncRNA lncRNA 4.156989 2.411447 6.612283 0.436097 0.1793087 1.451459 0.9066362 0.63621688 0.8858557 0.14335590 0.15248747 0.4712479 0.25553750 0.26330000 0.1333000 -0.13000000 0.04717771 0.02931751   FALSE
ENST00000654024 ENSG00000227354 HEK293_OSMI2_2hA HEK293_TMG_2hB RBM26-AS1 lncRNA lncRNA 4.156989 2.411447 6.612283 0.436097 0.1793087 1.451459 0.3793275 0.10419164 0.8839768 0.07263931 0.08671634 2.9687803 0.07559167 0.03666667 0.1338667 0.09720000 0.05599392 0.02931751 FALSE FALSE
ENST00000656910 ENSG00000227354 HEK293_OSMI2_2hA HEK293_TMG_2hB RBM26-AS1 lncRNA lncRNA 4.156989 2.411447 6.612283 0.436097 0.1793087 1.451459 0.7287202 0.38305147 1.6394355 0.03340350 0.22947686 2.0691822 0.17986250 0.16633333 0.2477333 0.08140000 0.33141723 0.02931751 FALSE TRUE
MSTRG.8948.16 ENSG00000227354 HEK293_OSMI2_2hA HEK293_TMG_2hB RBM26-AS1 lncRNA   4.156989 2.411447 6.612283 0.436097 0.1793087 1.451459 0.1743410 0.04197922 0.4432503 0.02232916 0.01854877 3.1243013 0.03477917 0.01473333 0.0671000 0.05236667 0.02931751 0.02931751   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000227354 E001 5.739206 0.0175793605 3.072666e-03 1.048030e-02 13 79406278 79406292 15 + 0.652 1.013 1.414
ENSG00000227354 E002 5.561768 0.0057422937 3.945765e-03 1.299052e-02 13 79406293 79406300 8 + 0.652 0.990 1.331
ENSG00000227354 E003 4.875749 0.0035060057 1.845265e-02 4.812565e-02 13 79406301 79406302 2 + 0.632 0.915 1.137
ENSG00000227354 E004 1.864719 0.0935998254 9.261055e-01 9.574708e-01 13 79406303 79406305 3 + 0.455 0.405 -0.264
ENSG00000227354 E005 1.719546 0.1605214661 9.242345e-01 9.562931e-01 13 79406306 79406308 3 + 0.423 0.405 -0.100
ENSG00000227354 E006 9.173031 0.0022250490 1.649905e-01 2.816855e-01 13 79406309 79406347 39 + 1.035 0.825 -0.795
ENSG00000227354 E007 11.056183 0.0018488470 4.561900e-01 5.966442e-01 13 79406348 79406363 16 + 1.089 0.966 -0.451
ENSG00000227354 E008 24.323578 0.0007983092 7.406331e-01 8.299954e-01 13 79406364 79406477 114 + 1.384 1.365 -0.064
ENSG00000227354 E009 7.875783 0.0045198028 9.778359e-02 1.871230e-01 13 79406478 79406537 60 + 0.855 1.013 0.595
ENSG00000227354 E010 6.763343 0.0056124322 8.356883e-02 1.651303e-01 13 79406538 79406543 6 + 0.788 0.967 0.689
ENSG00000227354 E011 7.354529 0.0029388243 1.788356e-01 2.996787e-01 13 79406544 79406561 18 + 0.842 0.967 0.474
ENSG00000227354 E012 6.228246 0.0107006768 2.962588e-01 4.374641e-01 13 79406562 79406571 10 + 0.787 0.888 0.393
ENSG00000227354 E013 7.712628 0.0040680361 1.431977e-01 2.523757e-01 13 79406572 79406628 57 + 0.855 0.991 0.513
ENSG00000227354 E014 5.135978 0.0682907271 1.045496e-01 1.974382e-01 13 79406629 79406657 29 + 0.652 0.897 0.981
ENSG00000227354 E015 11.000730 0.0020341793 2.748104e-01 4.140317e-01 13 79406658 79406783 126 + 1.019 1.092 0.267
ENSG00000227354 E016 6.723382 0.0046894483 6.363155e-06 4.425243e-05 13 79406784 79407095 312 + 0.611 1.127 2.010
ENSG00000227354 E017 17.490707 0.0010815965 2.025914e-01 3.294449e-01 13 79412682 79412831 150 + 1.285 1.127 -0.561
ENSG00000227354 E018 11.599900 0.0023937981 8.447856e-01 9.034117e-01 13 79412832 79412907 76 + 1.097 1.034 -0.229
ENSG00000227354 E019 2.427310 0.0064459028 5.508877e-01 6.804390e-01 13 79415111 79415261 151 + 0.485 0.554 0.331
ENSG00000227354 E020 6.492483 0.0026032782 7.547644e-01 8.403043e-01 13 79415262 79415364 103 + 0.855 0.857 0.008
ENSG00000227354 E021 17.492002 0.0011336078 3.460141e-01 4.901547e-01 13 79417835 79417916 82 + 1.285 1.159 -0.446
ENSG00000227354 E022 28.745190 0.0135699524 8.709896e-01 9.210495e-01 13 79420760 79420815 56 + 1.470 1.408 -0.212
ENSG00000227354 E023 54.603309 0.0005073155 7.968384e-01 8.703604e-01 13 79421987 79422195 209 + 1.730 1.699 -0.106
ENSG00000227354 E024 23.706504 0.0027988442 3.319232e-01 4.755545e-01 13 79422196 79422214 19 + 1.361 1.393 0.110
ENSG00000227354 E025 52.641221 0.0004451715 9.988514e-04 3.954059e-03 13 79422215 79422726 512 + 1.662 1.777 0.388
ENSG00000227354 E026 39.142331 0.0042526846 3.026526e-02 7.248626e-02 13 79422727 79423551 825 + 1.632 1.436 -0.671
ENSG00000227354 E027 106.849742 0.0405563043 7.225274e-04 2.979618e-03 13 79423552 79427317 3766 + 2.086 1.771 -1.062