ENSG00000227345

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000402038 ENSG00000227345 HEK293_OSMI2_2hA HEK293_TMG_2hB PARG protein_coding protein_coding 10.60084 4.009771 16.44456 0.1044866 0.337048 2.033302 0.1690555 0.62144903 0.000000 0.62144903 0.0000000 -5.980594 0.042629167 0.1474333 0.00000000 -0.14743333 0.59266320 0.001086963 FALSE TRUE
ENST00000614063 ENSG00000227345 HEK293_OSMI2_2hA HEK293_TMG_2hB PARG protein_coding nonsense_mediated_decay 10.60084 4.009771 16.44456 0.1044866 0.337048 2.033302 1.6562127 0.05639663 2.536947 0.05639663 0.3706001 5.261515 0.106850000 0.0143000 0.15516667 0.14086667 0.01280251 0.001086963 FALSE TRUE
ENST00000616448 ENSG00000227345 HEK293_OSMI2_2hA HEK293_TMG_2hB PARG protein_coding protein_coding 10.60084 4.009771 16.44456 0.1044866 0.337048 2.033302 5.3577826 2.78616693 7.135327 0.46689012 1.0535359 1.353550 0.545416667 0.7016333 0.43273333 -0.26890000 0.22397401 0.001086963 FALSE TRUE
MSTRG.3998.3 ENSG00000227345 HEK293_OSMI2_2hA HEK293_TMG_2hB PARG protein_coding   10.60084 4.009771 16.44456 0.1044866 0.337048 2.033302 0.1464727 0.00000000 1.171781 0.00000000 1.1717815 6.884819 0.009008333 0.0000000 0.07206667 0.07206667 1.00000000 0.001086963 TRUE TRUE
MSTRG.3998.4 ENSG00000227345 HEK293_OSMI2_2hA HEK293_TMG_2hB PARG protein_coding   10.60084 4.009771 16.44456 0.1044866 0.337048 2.033302 2.5939452 0.54575794 3.962557 0.03564330 0.5794317 2.837540 0.230645833 0.1366333 0.24190000 0.10526667 0.13948322 0.001086963 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000227345 E001 30.8796790 0.0135156337 1.697999e-11 3.404391e-10 10 49818279 49818501 223 - 1.218 1.789 1.962
ENSG00000227345 E002 72.3314347 0.0134711294 5.901282e-13 1.502056e-11 10 49818502 49818629 128 - 1.613 2.121 1.714
ENSG00000227345 E003 97.1758772 0.0074321089 4.184635e-13 1.090163e-11 10 49818630 49818889 260 - 1.797 2.199 1.350
ENSG00000227345 E004 62.2205898 0.0005327295 3.414432e-04 1.546013e-03 10 49818890 49818991 102 - 1.705 1.883 0.602
ENSG00000227345 E005 57.6201765 0.0016597577 2.513131e-02 6.214903e-02 10 49818992 49819066 75 - 1.691 1.817 0.426
ENSG00000227345 E006 40.6447358 0.0168335315 1.310964e-02 3.616324e-02 10 49819067 49819144 78 - 1.509 1.720 0.719
ENSG00000227345 E007 49.1348204 0.0005888422 1.275191e-05 8.266817e-05 10 49819145 49819275 131 - 1.582 1.818 0.802
ENSG00000227345 E008 17.4499546 0.0020658176 6.169502e-01 7.348869e-01 10 49819276 49819277 2 - 1.209 1.263 0.192
ENSG00000227345 E009 90.0203583 0.0028074935 6.280346e-01 7.436855e-01 10 49819278 49819494 217 - 1.910 1.942 0.108
ENSG00000227345 E010 45.5721553 0.0014546194 3.748979e-01 5.193384e-01 10 49820165 49820209 45 - 1.613 1.675 0.211
ENSG00000227345 E011 63.1006597 0.0005268876 2.766972e-01 4.161695e-01 10 49820210 49820293 84 - 1.748 1.811 0.212
ENSG00000227345 E012 70.8346529 0.0003984584 9.632652e-01 9.808003e-01 10 49832803 49832908 106 - 1.813 1.822 0.029
ENSG00000227345 E013 1.1823723 0.0108756036 5.850637e-02 1.240774e-01 10 49832909 49833274 366 - 0.401 0.000 -11.469
ENSG00000227345 E014 73.7165910 0.0003945294 2.635628e-01 4.014826e-01 10 49841950 49842058 109 - 1.844 1.803 -0.137
ENSG00000227345 E015 66.1070352 0.0003580695 2.676579e-01 4.059904e-01 10 49843554 49843632 79 - 1.798 1.755 -0.146
ENSG00000227345 E016 0.0000000       10 49857295 49857305 11 -      
ENSG00000227345 E017 82.2858818 0.0015641026 3.119957e-01 4.545162e-01 10 49857306 49857453 148 - 1.891 1.854 -0.125
ENSG00000227345 E018 49.9292466 0.0064560094 4.311982e-01 5.737729e-01 10 49861588 49861663 76 - 1.679 1.633 -0.158
ENSG00000227345 E019 34.3292263 0.0006648149 2.016953e-01 3.282920e-01 10 49865321 49865381 61 - 1.529 1.453 -0.260
ENSG00000227345 E020 0.5827671 0.1136304264 3.031228e-01 4.450254e-01 10 49867072 49867434 363 - 0.245 0.000 -10.466
ENSG00000227345 E021 45.7642670 0.0004782896 1.663177e-02 4.412631e-02 10 49869476 49869555 80 - 1.664 1.531 -0.453
ENSG00000227345 E022 68.9214110 0.0003618734 5.376739e-03 1.695277e-02 10 49879673 49879830 158 - 1.836 1.710 -0.424
ENSG00000227345 E023 42.7530229 0.0005487061 9.857777e-04 3.910231e-03 10 49885203 49885295 93 - 1.651 1.453 -0.678
ENSG00000227345 E024 0.5932625 0.1680550014 3.177726e-01 4.606614e-01 10 49908455 49908541 87 - 0.245 0.000 -10.469
ENSG00000227345 E025 45.3563386 0.0010360636 1.725101e-04 8.459700e-04 10 49915917 49915991 75 - 1.681 1.453 -0.780
ENSG00000227345 E026 35.9377364 0.0025342417 1.955858e-03 7.089340e-03 10 49922336 49922419 84 - 1.577 1.358 -0.753
ENSG00000227345 E027 50.7677662 0.0005347054 1.402377e-02 3.828016e-02 10 49922547 49922669 123 - 1.708 1.578 -0.441
ENSG00000227345 E028 79.0494167 0.0044775540 4.190200e-02 9.454637e-02 10 49932100 49932283 184 - 1.890 1.784 -0.356
ENSG00000227345 E029 219.8578628 0.0091676672 6.191447e-06 4.317240e-05 10 49933177 49934163 987 - 2.356 2.130 -0.755
ENSG00000227345 E030 30.3703886 0.0049955513 2.986665e-04 1.374237e-03 10 49935076 49935142 67 - 1.521 1.222 -1.040
ENSG00000227345 E031 52.8002142 0.0112968771 8.688539e-05 4.604761e-04 10 49941509 49941785 277 - 1.757 1.448 -1.053
ENSG00000227345 E032 25.9522855 0.0111765809 3.571236e-03 1.193122e-02 10 49941786 49941824 39 - 1.454 1.176 -0.973
ENSG00000227345 E033 32.0190552 0.0054481067 6.320415e-03 1.945826e-02 10 49941825 49941934 110 - 1.529 1.314 -0.743
ENSG00000227345 E034 17.9831189 0.0024240100 8.423901e-02 1.661980e-01 10 49941935 49942027 93 - 1.279 1.124 -0.551
ENSG00000227345 E035 0.0000000       10 49970167 49970203 37 -