ENSG00000227124

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000477374 ENSG00000227124 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF717 protein_coding protein_coding 9.147917 10.66915 9.618108 0.9229554 0.30109 -0.149473 5.9596350 8.33311258 5.5251616 0.88046494 0.3168352 -0.59196028 0.6396083 0.778566667 0.5735000 -0.20506667 1.135075e-05 1.135075e-05 FALSE TRUE
ENST00000648506 ENSG00000227124 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF717 protein_coding processed_transcript 9.147917 10.66915 9.618108 0.9229554 0.30109 -0.149473 0.8478259 0.92965857 0.9522683 0.11793960 0.1130099 0.03430254 0.0938750 0.086666667 0.0995000 0.01283333 8.449469e-01 1.135075e-05   FALSE
ENST00000652011 ENSG00000227124 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF717 protein_coding protein_coding 9.147917 10.66915 9.618108 0.9229554 0.30109 -0.149473 0.8558377 0.06985471 1.4556393 0.06985471 0.1404376 4.19800883 0.1067417 0.005633333 0.1507333 0.14510000 3.786083e-05 1.135075e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000227124 E001 9.763995 0.0056678599 2.177258e-01 3.476577e-01 3 75678660 75679151 492 - 1.125 0.952 -0.631
ENSG00000227124 E002 7.535645 0.0300912839 1.096126e-03 4.291069e-03 3 75709643 75711186 1544 - 1.160 0.661 -1.908
ENSG00000227124 E003 4.527567 0.0038429372 1.243934e-01 2.262438e-01 3 75711187 75711316 130 - 0.878 0.633 -0.990
ENSG00000227124 E004 8.848278 0.0052225922 3.546830e-02 8.255455e-02 3 75716419 75716541 123 - 0.855 1.058 0.757
ENSG00000227124 E005 67.666186 0.0010312682 9.573448e-07 7.972838e-06 3 75729961 75730195 235 - 1.715 1.889 0.589
ENSG00000227124 E006 57.376486 0.0005018232 1.743806e-07 1.691285e-06 3 75730196 75730252 57 - 1.621 1.826 0.691
ENSG00000227124 E007 67.505288 0.0012558915 3.423207e-08 3.812335e-07 3 75730253 75730323 71 - 1.688 1.897 0.704
ENSG00000227124 E008 62.258519 0.0005231225 7.527524e-10 1.142773e-08 3 75730324 75730374 51 - 1.629 1.869 0.812
ENSG00000227124 E009 93.047873 0.0006745484 5.219248e-04 2.241800e-03 3 75730375 75730618 244 - 1.911 1.993 0.276
ENSG00000227124 E010 37.568292 0.0007511618 6.239164e-01 7.403821e-01 3 75732060 75732121 62 - 1.588 1.571 -0.057
ENSG00000227124 E011 1.179264 0.0132248542 1.164883e-02 3.269757e-02 3 75735868 75735874 7 - 0.568 0.105 -3.294
ENSG00000227124 E012 73.986434 0.0186167157 3.112542e-22 2.542741e-20 3 75735875 75739291 3417 - 2.185 1.386 -2.706
ENSG00000227124 E013 3.147326 0.0049841095 9.561056e-06 6.384383e-05 3 75739292 75739345 54 - 0.920 0.190 -3.734
ENSG00000227124 E014 60.462266 0.0056722393 9.843162e-01 9.942828e-01 3 75741276 75741368 93 - 1.805 1.754 -0.170
ENSG00000227124 E015 51.097550 0.0009124253 9.791637e-01 9.910333e-01 3 75741610 75741736 127 - 1.730 1.686 -0.150
ENSG00000227124 E016 7.979589 0.0080138241 5.968692e-01 7.187279e-01 3 75741737 75741865 129 - 1.011 0.910 -0.376
ENSG00000227124 E017 31.452761 0.0007969049 2.594900e-01 3.968837e-01 3 75783306 75783364 59 - 1.571 1.459 -0.384
ENSG00000227124 E018 15.186059 0.0096431443 4.032174e-01 5.470024e-01 3 75784784 75784885 102 - 1.272 1.151 -0.429
ENSG00000227124 E019 7.820773 0.0022586086 8.206687e-01 8.869132e-01 3 75784886 75784914 29 - 0.976 0.911 -0.246
ENSG00000227124 E020 15.788192 0.0011977145 4.727361e-01 6.117839e-01 3 75784915 75785002 88 - 1.272 1.169 -0.366
ENSG00000227124 E021 8.295227 0.0022481268 6.019410e-01 7.229845e-01 3 75785003 75785069 67 - 1.010 0.911 -0.370
ENSG00000227124 E022 11.546606 0.0016450001 3.406236e-01 4.845807e-01 3 75785070 75785336 267 - 1.161 1.026 -0.486
ENSG00000227124 E023 37.472474 0.0013243934 3.004249e-02 7.203923e-02 3 75785384 75785583 200 - 1.671 1.502 -0.574