ENSG00000226266

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000418770 ENSG00000226266 HEK293_OSMI2_2hA HEK293_TMG_2hB lncRNA lncRNA 0.6688801 0.4359477 0.6235689 0.1392128 0.07536986 0.5066268 0.14097494 0.0000000 0.19788325 0.00000000 0.11371737 4.3777016 0.2422375 0.0000 0.36866667 0.36866667 3.932972e-01 4.582016e-10   FALSE
ENST00000453016 ENSG00000226266 HEK293_OSMI2_2hA HEK293_TMG_2hB lncRNA lncRNA 0.6688801 0.4359477 0.6235689 0.1392128 0.07536986 0.5066268 0.37409795 0.0000000 0.36924075 0.00000000 0.19117086 5.2450421 0.3621083 0.0000 0.53246667 0.53246667 3.014131e-01 4.582016e-10   FALSE
ENST00000607836 ENSG00000226266 HEK293_OSMI2_2hA HEK293_TMG_2hB lncRNA lncRNA 0.6688801 0.4359477 0.6235689 0.1392128 0.07536986 0.5066268 0.05480963 0.0326852 0.05644486 0.01230382 0.01349782 0.6384216 0.1374958 0.0839 0.09886667 0.01496667 9.879387e-01 4.582016e-10   FALSE
ENST00000693353 ENSG00000226266 HEK293_OSMI2_2hA HEK293_TMG_2hB lncRNA lncRNA 0.6688801 0.4359477 0.6235689 0.1392128 0.07536986 0.5066268 0.09899757 0.4032625 0.00000000 0.13277779 0.00000000 -5.3689866 0.2581583 0.9161 0.00000000 -0.91610000 4.582016e-10 4.582016e-10   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000226266 E001 0.7512344 0.046213934 0.06806498 0.14015376 2 159670708 159670711 4 - 0.386 0.000 -12.833
ENSG00000226266 E002 1.0499988 0.027056520 0.02063885 0.05282619 2 159670712 159670810 99 - 0.478 0.000 -13.318
ENSG00000226266 E003 0.1472490 0.051635378 0.84532398   2 159670811 159670813 3 - 0.109 0.000 -10.510
ENSG00000226266 E004 2.0994008 0.009173890 0.90785274 0.94552459 2 159693201 159695420 2220 - 0.517 0.455 -0.307
ENSG00000226266 E005 0.3697384 0.028088908 0.08581112 0.16869004 2 159710619 159710716 98 - 0.000 0.241 11.977
ENSG00000226266 E006 4.7294049 0.004349458 0.26757421 0.40590248 2 159710717 159710894 178 - 0.747 0.764 0.071
ENSG00000226266 E007 0.2944980 0.418472316 0.52850460   2 159711921 159712018 98 - 0.196 0.000 -11.508
ENSG00000226266 E008 2.3260429 0.007167978 0.27527821 0.41456181 2 159712314 159712444 131 - 0.478 0.555 0.371