ENSG00000225975

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000425254 ENSG00000225975 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF567-DT lncRNA lncRNA 1.903868 2.544874 1.361108 0.2928956 0.06289359 -0.8979101 0.05116495 0.13788761 0.00000000 0.137887615 0.00000000 -3.8864293 0.02390833 0.06313333 0.0000000 -0.06313333 0.89506310 0.03910547    
ENST00000433232 ENSG00000225975 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF567-DT lncRNA lncRNA 1.903868 2.544874 1.361108 0.2928956 0.06289359 -0.8979101 1.46367462 1.97795650 1.04003310 0.265400182 0.10021470 -0.9208514 0.73557917 0.77443333 0.7607333 -0.01370000 0.97498406 0.03910547    
ENST00000590952 ENSG00000225975 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF567-DT lncRNA lncRNA 1.903868 2.544874 1.361108 0.2928956 0.06289359 -0.8979101 0.10248268 0.24450231 0.06417648 0.124380638 0.06417648 -1.7786450 0.05720000 0.09023333 0.0490000 -0.04123333 0.71569118 0.03910547    
ENST00000665321 ENSG00000225975 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF567-DT lncRNA lncRNA 1.903868 2.544874 1.361108 0.2928956 0.06289359 -0.8979101 0.19710219 0.10111893 0.18560045 0.009176538 0.03418438 0.8158051 0.12856667 0.04140000 0.1349000 0.09350000 0.03910547 0.03910547    
ENST00000690752 ENSG00000225975 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF567-DT lncRNA lncRNA 1.903868 2.544874 1.361108 0.2928956 0.06289359 -0.8979101 0.08944343 0.08340819 0.07129758 0.046319315 0.07129758 -0.2003366 0.05474167 0.03083333 0.0554000 0.02456667 0.92053542 0.03910547    
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000225975 E001 5.3869676 0.007955159 7.624728e-09 9.604840e-08 19 36682636 36685309 2674 - 1.145 0.384 -3.186
ENSG00000225975 E002 0.7321030 0.120168610 6.423630e-01 7.551656e-01 19 36685310 36685439 130 - 0.160 0.235 0.688
ENSG00000225975 E003 0.6558305 0.178380963 6.050242e-01 7.254524e-01 19 36685440 36685511 72 - 0.277 0.169 -0.905
ENSG00000225975 E004 0.8459408 0.016438772 8.431293e-01 9.022823e-01 19 36685512 36685716 205 - 0.277 0.233 -0.327
ENSG00000225975 E005 0.1472490 0.043306646 2.780018e-01   19 36685717 36685721 5 - 0.160 0.000 -9.157
ENSG00000225975 E006 9.1096242 0.002146932 1.356614e-01 2.420411e-01 19 36685722 36685836 115 - 0.860 1.011 0.566
ENSG00000225975 E007 19.9851821 0.001087232 2.116888e-01 3.404745e-01 19 36685837 36686015 179 - 1.243 1.315 0.252
ENSG00000225975 E008 1.3618961 0.010510184 5.243085e-01 6.578687e-01 19 36686016 36686190 175 - 0.277 0.384 0.673
ENSG00000225975 E009 2.2077497 0.016752758 6.062554e-01 7.264493e-01 19 36686191 36686685 495 - 0.445 0.526 0.403
ENSG00000225975 E010 18.7382552 0.001085467 2.759313e-01 4.153366e-01 19 36686686 36686806 121 - 1.221 1.285 0.224
ENSG00000225975 E011 1.5508631 0.029078516 3.661840e-01 5.105271e-01 19 36686807 36687267 461 - 0.509 0.339 -0.919
ENSG00000225975 E012 12.1007744 0.001530828 6.082547e-01 7.280466e-01 19 36687268 36687468 201 - 1.069 1.101 0.116