ENSG00000225830

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000355832 ENSG00000225830 HEK293_OSMI2_2hA HEK293_TMG_2hB ERCC6 protein_coding protein_coding 4.608276 2.224853 6.804555 0.08164868 0.1660026 1.60844 0.42515115 0.16654079 0.8197610 0.094709831 0.05947976 2.232694 0.07865000 0.07190000 0.120266667 0.04836667 5.724587e-01 1.013859e-11 FALSE TRUE
ENST00000447839 ENSG00000225830 HEK293_OSMI2_2hA HEK293_TMG_2hB ERCC6 protein_coding protein_coding 4.608276 2.224853 6.804555 0.08164868 0.1660026 1.60844 0.99783726 0.37242049 1.6198632 0.187995273 0.20273452 2.091519 0.21098750 0.16163333 0.239666667 0.07803333 7.233657e-01 1.013859e-11 FALSE TRUE
ENST00000475116 ENSG00000225830 HEK293_OSMI2_2hA HEK293_TMG_2hB ERCC6 protein_coding processed_transcript 4.608276 2.224853 6.804555 0.08164868 0.1660026 1.60844 0.10125038 0.00000000 0.4093915 0.000000000 0.20474692 5.390226 0.02000417 0.00000000 0.058800000 0.05880000 3.646376e-01 1.013859e-11 FALSE FALSE
ENST00000624341 ENSG00000225830 HEK293_OSMI2_2hA HEK293_TMG_2hB ERCC6 protein_coding nonsense_mediated_decay 4.608276 2.224853 6.804555 0.08164868 0.1660026 1.60844 0.57615023 0.63411246 0.2992411 0.022579097 0.02649857 -1.058580 0.16241250 0.28506667 0.043866667 -0.24120000 1.013859e-11 1.013859e-11 FALSE TRUE
ENST00000679596 ENSG00000225830 HEK293_OSMI2_2hA HEK293_TMG_2hB ERCC6 protein_coding nonsense_mediated_decay 4.608276 2.224853 6.804555 0.08164868 0.1660026 1.60844 0.03059557 0.13762048 0.0567063 0.137620484 0.05670630 -1.145998 0.01026667 0.06643333 0.008033333 -0.05840000 8.543960e-01 1.013859e-11 FALSE TRUE
ENST00000680233 ENSG00000225830 HEK293_OSMI2_2hA HEK293_TMG_2hB ERCC6 protein_coding retained_intron 4.608276 2.224853 6.804555 0.08164868 0.1660026 1.60844 0.24779602 0.06389608 0.4135461 0.008917264 0.05008279 2.518949 0.05175833 0.02863333 0.060666667 0.03203333 3.631318e-01 1.013859e-11 FALSE TRUE
MSTRG.3964.9 ENSG00000225830 HEK293_OSMI2_2hA HEK293_TMG_2hB ERCC6 protein_coding   4.608276 2.224853 6.804555 0.08164868 0.1660026 1.60844 1.62245247 0.60921948 2.0640355 0.106786088 0.09061837 1.743918 0.33536250 0.27533333 0.303966667 0.02863333 8.304654e-01 1.013859e-11 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000225830 E001 3.4709411 0.0502490291 1.576063e-01 2.719312e-01 10 49454168 49454469 302 - 0.691 0.408 -1.323
ENSG00000225830 E002 9.5687157 0.0017859278 8.276429e-01 8.916477e-01 10 49454470 49455367 898 - 0.993 1.011 0.068
ENSG00000225830 E003 27.5708647 0.0359686797 8.608260e-01 9.142382e-01 10 49455368 49456359 992 - 1.424 1.426 0.008
ENSG00000225830 E004 12.3997369 0.0015114887 9.178603e-04 3.677245e-03 10 49456360 49456654 295 - 0.984 1.291 1.101
ENSG00000225830 E005 46.4254299 0.0014913120 1.413009e-09 2.037837e-08 10 49456655 49457472 818 - 1.528 1.841 1.063
ENSG00000225830 E006 63.9421785 0.0004705883 1.351428e-03 5.148074e-03 10 49457473 49458787 1315 - 1.736 1.873 0.461
ENSG00000225830 E007 43.3648558 0.0005362157 7.798428e-07 6.630711e-06 10 49458788 49459234 447 - 1.520 1.776 0.871
ENSG00000225830 E008 0.6235652 0.0247030358 9.150307e-01 9.502098e-01 10 49459235 49460023 789 - 0.209 0.180 -0.260
ENSG00000225830 E009 1.4737724 0.0094822019 3.062838e-01 4.484366e-01 10 49460024 49460372 349 - 0.307 0.486 1.003
ENSG00000225830 E010 19.6239254 0.0018851617 1.033671e-02 2.959795e-02 10 49460373 49460451 79 - 1.221 1.418 0.689
ENSG00000225830 E011 29.9496617 0.0011751505 1.158828e-02 3.255671e-02 10 49461352 49461556 205 - 1.410 1.568 0.545
ENSG00000225830 E012 72.2997070 0.0003799804 9.174596e-03 2.671903e-02 10 49470182 49470889 708 - 1.804 1.907 0.347
ENSG00000225830 E013 19.3062216 0.0065362725 3.588700e-01 5.032404e-01 10 49470975 49471120 146 - 1.252 1.325 0.256
ENSG00000225830 E014 0.0000000       10 49471511 49471651 141 -      
ENSG00000225830 E015 18.6393201 0.0209746108 9.274157e-01 9.583233e-01 10 49472376 49472470 95 - 1.267 1.247 -0.070
ENSG00000225830 E016 0.1515154 0.0436771233 1.000000e+00   10 49472471 49472908 438 - 0.081 0.000 -10.308
ENSG00000225830 E017 21.3320736 0.0014711651 4.401364e-01 5.818314e-01 10 49472909 49473028 120 - 1.338 1.268 -0.245
ENSG00000225830 E018 0.3289534 0.0297259583 4.928637e-01   10 49473029 49473476 448 - 0.081 0.180 1.325
ENSG00000225830 E019 22.0433131 0.0011020338 6.466867e-01 7.585104e-01 10 49473477 49473587 111 - 1.325 1.355 0.102
ENSG00000225830 E020 0.7384352 0.0168059811 1.441362e-01 2.536335e-01 10 49473588 49474026 439 - 0.307 0.000 -12.510
ENSG00000225830 E021 25.6806598 0.0083732362 3.675590e-01 5.119280e-01 10 49474027 49474242 216 - 1.427 1.344 -0.286
ENSG00000225830 E022 0.5181333 0.0212817852 8.388460e-01 8.992732e-01 10 49475327 49475496 170 - 0.150 0.181 0.326
ENSG00000225830 E023 11.9281535 0.0057671155 1.074622e-02 3.055943e-02 10 49476215 49476310 96 - 1.164 0.856 -1.135
ENSG00000225830 E024 15.1097141 0.0018805023 3.229500e-01 4.663113e-01 10 49478354 49478470 117 - 1.210 1.109 -0.362
ENSG00000225830 E025 18.3873779 0.0010522863 3.881872e-02 8.884755e-02 10 49482687 49482863 177 - 1.317 1.126 -0.676
ENSG00000225830 E026 9.0664065 0.0028906650 1.463743e-01 2.567467e-01 10 49483346 49483367 22 - 1.036 0.856 -0.677
ENSG00000225830 E027 9.4095274 0.0314464154 5.178875e-01 6.522143e-01 10 49483368 49483385 18 - 1.028 0.937 -0.339
ENSG00000225830 E028 18.1274635 0.0015305472 4.488600e-02 9.999668e-02 10 49483386 49483516 131 - 1.313 1.126 -0.662
ENSG00000225830 E029 0.0000000       10 49488158 49488379 222 -      
ENSG00000225830 E030 18.3949897 0.0010546511 2.471352e-01 3.825653e-01 10 49493117 49493252 136 - 1.295 1.188 -0.377
ENSG00000225830 E031 19.7318195 0.0024017538 5.205675e-02 1.128155e-01 10 49500538 49500659 122 - 1.338 1.158 -0.633
ENSG00000225830 E032 13.9231851 0.0030943105 5.645176e-01 6.918052e-01 10 49500660 49500696 37 - 1.125 1.174 0.174
ENSG00000225830 E033 0.9651910 0.0136968632 7.864480e-01 8.632410e-01 10 49500697 49502037 1341 - 0.261 0.308 0.324
ENSG00000225830 E034 1.1888288 0.0135930152 4.339828e-02 9.729216e-02 10 49502038 49504991 2954 - 0.423 0.000 -13.121
ENSG00000225830 E035 0.2924217 0.0273789737 6.132611e-01   10 49504992 49505883 892 - 0.150 0.000 -11.274
ENSG00000225830 E036 21.2648729 0.0008446535 9.103502e-01 9.470192e-01 10 49505884 49506012 129 - 1.321 1.324 0.009
ENSG00000225830 E037 0.6717251 0.0308706054 9.181664e-01 9.522770e-01 10 49506013 49506480 468 - 0.209 0.181 -0.258
ENSG00000225830 E038 4.2295598 0.1464293706 9.313480e-01 9.607559e-01 10 49514114 49515197 1084 - 0.692 0.673 -0.080
ENSG00000225830 E039 0.2987644 0.0271845674 6.132390e-01   10 49515198 49515200 3 - 0.150 0.000 -11.274
ENSG00000225830 E040 1.5542001 0.3206384060 6.930252e-01 7.941229e-01 10 49515201 49515233 33 - 0.423 0.309 -0.671
ENSG00000225830 E041 61.1238899 0.0004627382 9.361308e-01 9.638245e-01 10 49515234 49517121 1888 - 1.771 1.761 -0.034
ENSG00000225830 E042 10.2266025 0.0018977185 5.162120e-02 1.120395e-01 10 49522289 49524032 1744 - 1.090 0.856 -0.872
ENSG00000225830 E043 75.3501411 0.0061698385 1.374024e-02 3.763101e-02 10 49524033 49524777 745 - 1.900 1.754 -0.492
ENSG00000225830 E044 2.0315474 0.0069450262 8.430557e-01 9.022391e-01 10 49524936 49525226 291 - 0.455 0.486 0.154
ENSG00000225830 E045 21.9851879 0.0174546526 5.713279e-03 1.785313e-02 10 49528417 49528525 109 - 1.416 1.122 -1.036
ENSG00000225830 E046 11.4700993 0.0114958852 1.106059e-01 2.064246e-01 10 49530720 49530723 4 - 1.132 0.939 -0.708
ENSG00000225830 E047 23.4390523 0.0008514103 1.703499e-03 6.294137e-03 10 49530724 49530840 117 - 1.437 1.173 -0.922
ENSG00000225830 E048 47.8175561 0.0004895352 5.098413e-05 2.867471e-04 10 49532543 49532974 432 - 1.730 1.489 -0.818
ENSG00000225830 E049 9.6877070 0.0071196879 9.977418e-03 2.870843e-02 10 49532975 49532978 4 - 1.090 0.751 -1.287
ENSG00000225830 E050 15.3071022 0.0014658750 2.374713e-01 3.712278e-01 10 49538962 49539121 160 - 1.226 1.108 -0.420
ENSG00000225830 E051 0.0000000       10 49539432 49539538 107 -