ENSG00000225733

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000417835 ENSG00000225733 HEK293_OSMI2_2hA HEK293_TMG_2hB FGD5-AS1 lncRNA lncRNA 33.84163 13.90186 59.85466 1.476909 2.050115 2.105389 3.361701 0.09678377 8.525273 0.09678377 0.54191650 6.3206731 0.07177083 0.00690000 0.14226667 0.13536667 0.003290454 0.003290454 FALSE FALSE
ENST00000424349 ENSG00000225733 HEK293_OSMI2_2hA HEK293_TMG_2hB FGD5-AS1 lncRNA lncRNA 33.84163 13.90186 59.85466 1.476909 2.050115 2.105389 9.501602 8.10786089 14.169215 0.85453196 8.28603935 0.8046061 0.37045000 0.58636667 0.23240000 -0.35396667 0.074005311 0.003290454   FALSE
ENST00000655923 ENSG00000225733 HEK293_OSMI2_2hA HEK293_TMG_2hB FGD5-AS1 lncRNA lncRNA 33.84163 13.90186 59.85466 1.476909 2.050115 2.105389 6.738569 0.00000000 13.369064 0.00000000 6.68575961 10.3857615 0.12760833 0.00000000 0.22760000 0.22760000 0.272837587 0.003290454   FALSE
ENST00000665449 ENSG00000225733 HEK293_OSMI2_2hA HEK293_TMG_2hB FGD5-AS1 lncRNA lncRNA 33.84163 13.90186 59.85466 1.476909 2.050115 2.105389 7.775488 3.03256479 14.872613 0.40912671 1.36906703 2.2902680 0.23397083 0.21683333 0.24810000 0.03126667 0.631831153 0.003290454   FALSE
ENST00000665738 ENSG00000225733 HEK293_OSMI2_2hA HEK293_TMG_2hB FGD5-AS1 lncRNA lncRNA 33.84163 13.90186 59.85466 1.476909 2.050115 2.105389 2.310133 1.16472109 3.202186 0.62768266 0.78923718 1.4512372 0.06748333 0.07840000 0.05456667 -0.02383333 0.995645642 0.003290454   FALSE
ENST00000693748 ENSG00000225733 HEK293_OSMI2_2hA HEK293_TMG_2hB FGD5-AS1 lncRNA lncRNA 33.84163 13.90186 59.85466 1.476909 2.050115 2.105389 2.640003 1.21204709 3.812684 0.13196430 0.05922274 1.6452860 0.09383333 0.09093333 0.06390000 -0.02703333 0.457847177 0.003290454   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000225733 E001 1.142626 0.0130370885 5.500010e-05 3.066754e-04 3 14920347 14920483 137 - 0.000 0.679 13.830
ENSG00000225733 E002 2.925660 0.0052195315 1.352336e-02 3.713469e-02 3 14921309 14921459 151 - 0.401 0.823 1.897
ENSG00000225733 E003 2.559144 0.0060236470 1.944297e-01 3.193194e-01 3 14922301 14922404 104 - 0.429 0.678 1.160
ENSG00000225733 E004 4.060800 0.0042139069 1.881066e-03 6.856155e-03 3 14923091 14923348 258 - 0.480 0.962 2.013
ENSG00000225733 E005 2.237095 0.0095023556 4.755442e-04 2.066578e-03 3 14923977 14924098 122 - 0.224 0.823 3.069
ENSG00000225733 E006 3.456954 0.0047542059 3.367757e-06 2.493723e-05 3 14942784 14942788 5 - 0.303 1.018 3.218
ENSG00000225733 E007 7.029835 0.0025995424 1.374056e-10 2.370124e-09 3 14942789 14942796 8 - 0.504 1.297 3.103
ENSG00000225733 E008 1171.047426 0.0090081501 4.000223e-06 2.910255e-05 3 14942797 14944266 1470 - 2.947 3.143 0.650
ENSG00000225733 E009 391.098777 0.0001430454 1.989314e-01 3.248708e-01 3 14944267 14944679 413 - 2.512 2.580 0.227
ENSG00000225733 E010 94.786334 0.0003281781 4.708185e-01 6.100232e-01 3 14944680 14944692 13 - 1.901 1.973 0.243
ENSG00000225733 E011 357.100096 0.0001648636 9.393163e-04 3.751700e-03 3 14944693 14945023 331 - 2.497 2.468 -0.099
ENSG00000225733 E012 1161.147376 0.0027974666 3.839224e-13 1.005293e-11 3 14945024 14946154 1131 - 3.027 2.904 -0.411
ENSG00000225733 E013 75.582547 0.0147117802 2.816677e-02 6.834111e-02 3 14946204 14947086 883 - 1.765 1.955 0.643
ENSG00000225733 E014 63.956433 0.0010350847 2.403933e-02 5.989661e-02 3 14947087 14947109 23 - 1.769 1.688 -0.277
ENSG00000225733 E015 50.309596 0.0025190710 1.153880e-01 2.134132e-01 3 14947110 14947113 4 - 1.661 1.601 -0.203
ENSG00000225733 E016 89.623442 0.0004474928 3.476854e-03 1.165804e-02 3 14947114 14947209 96 - 1.913 1.822 -0.309
ENSG00000225733 E017 92.194291 0.0092257141 8.273287e-03 2.447492e-02 3 14947210 14947538 329 - 1.934 1.794 -0.471
ENSG00000225733 E018 11.208122 0.0015076543 8.496715e-04 3.436544e-03 3 14947739 14948012 274 - 1.096 0.679 -1.605
ENSG00000225733 E019 48.393226 0.0021488399 2.470451e-05 1.500881e-04 3 14948013 14948424 412 - 1.679 1.427 -0.859