ENSG00000225663

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000455127 ENSG00000225663 HEK293_OSMI2_2hA HEK293_TMG_2hB MCRIP1 protein_coding protein_coding 154.0107 310.8569 63.87217 10.17325 2.425281 -2.282812 90.969570 189.47125 31.429986 5.302920 0.5192837 -2.5913826 0.56497083 0.60996667 0.4938333 -0.11613333 0.01314004 0.004783007 FALSE FALSE
ENST00000538396 ENSG00000225663 HEK293_OSMI2_2hA HEK293_TMG_2hB MCRIP1 protein_coding protein_coding 154.0107 310.8569 63.87217 10.17325 2.425281 -2.282812 14.272113 25.24756 8.066588 1.751951 0.9030957 -1.6448973 0.09580000 0.08173333 0.1272333 0.04550000 0.16244890 0.004783007 FALSE FALSE
ENST00000573478 ENSG00000225663 HEK293_OSMI2_2hA HEK293_TMG_2hB MCRIP1 protein_coding protein_coding 154.0107 310.8569 63.87217 10.17325 2.425281 -2.282812 26.621063 56.13126 9.083754 1.072100 0.3186409 -2.6261134 0.16788333 0.18116667 0.1429333 -0.03823333 0.18639974 0.004783007 FALSE FALSE
MSTRG.15294.1 ENSG00000225663 HEK293_OSMI2_2hA HEK293_TMG_2hB MCRIP1 protein_coding   154.0107 310.8569 63.87217 10.17325 2.425281 -2.282812 9.624278 16.38528 10.987292 8.194195 3.2971092 -0.5761327 0.08880833 0.05146667 0.1687333 0.11726667 0.61248396 0.004783007 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000225663 E001 2.470935 6.625092e-02 9.196858e-02 1.781802e-01 17 81822143 81822188 46 - 0.001 0.466 9.618
ENSG00000225663 E002 3.016137 1.011805e-02 7.318809e-01 8.234605e-01 17 81822361 81822416 56 - 0.418 0.490 0.368
ENSG00000225663 E003 111.966256 7.322600e-04 6.705538e-02 1.384692e-01 17 81822417 81822428 12 - 1.938 1.850 -0.297
ENSG00000225663 E004 210.211700 1.849399e-04 9.852314e-01 9.947830e-01 17 81822429 81822453 25 - 2.134 2.135 0.002
ENSG00000225663 E005 2046.780967 1.401807e-03 3.017496e-02 7.231204e-02 17 81822454 81822800 347 - 3.083 3.126 0.143
ENSG00000225663 E006 759.288064 2.014205e-03 6.302332e-01 7.454363e-01 17 81822801 81822804 4 - 2.678 2.693 0.052
ENSG00000225663 E007 1105.072482 1.500625e-03 1.685994e-01 2.863425e-01 17 81822805 81822863 59 - 2.824 2.858 0.113
ENSG00000225663 E008 1031.682628 5.598893e-04 1.477048e-02 3.997518e-02 17 81822864 81822907 44 - 2.782 2.830 0.161
ENSG00000225663 E009 1805.559769 9.642052e-05 1.494585e-07 1.467603e-06 17 81822908 81823116 209 - 3.005 3.073 0.226
ENSG00000225663 E010 673.677900 1.007535e-04 2.055082e-02 5.265189e-02 17 81823117 81823125 9 - 2.595 2.643 0.161
ENSG00000225663 E011 1153.663022 1.081007e-04 4.820921e-03 1.543299e-02 17 81823126 81823208 83 - 2.831 2.877 0.151
ENSG00000225663 E012 1485.918136 8.059459e-05 4.787148e-02 1.053959e-01 17 81823209 81823311 103 - 3.004 2.978 -0.088
ENSG00000225663 E013 8.218431 2.463329e-03 2.182764e-02 5.531792e-02 17 81823312 81823411 100 - 1.061 0.756 -1.160
ENSG00000225663 E014 1302.697046 1.970548e-04 4.285709e-03 1.394252e-02 17 81823412 81823513 102 - 2.961 2.918 -0.143
ENSG00000225663 E015 2.033619 2.717501e-02 6.365035e-01 7.505163e-01 17 81823678 81823820 143 - 0.258 0.364 0.698
ENSG00000225663 E016 1402.646467 2.810967e-04 8.859251e-04 3.564832e-03 17 81824287 81824390 104 - 2.999 2.949 -0.167
ENSG00000225663 E017 737.523484 1.289900e-03 8.427079e-04 3.411323e-03 17 81824391 81824405 15 - 2.749 2.666 -0.275
ENSG00000225663 E018 53.483117 4.323150e-04 5.789177e-01 7.039017e-01 17 81824406 81824487 82 - 1.513 1.553 0.136
ENSG00000225663 E019 858.501496 1.404982e-03 9.462450e-04 3.775633e-03 17 81824499 81824554 56 - 2.812 2.732 -0.266
ENSG00000225663 E020 61.269750 2.031662e-03 3.533765e-04 1.593315e-03 17 81824555 81826379 1825 - 1.796 1.576 -0.748
ENSG00000225663 E021 7.475842 2.275412e-03 9.948694e-01 1.000000e+00 17 81826380 81826515 136 - 0.768 0.767 -0.004
ENSG00000225663 E022 5.199056 1.426072e-01 5.354078e-01 6.672534e-01 17 81826516 81826662 147 - 0.767 0.617 -0.625
ENSG00000225663 E023 432.657416 3.330968e-03 2.008518e-02 5.166783e-02 17 81833238 81833302 65 - 2.519 2.434 -0.282